975 research outputs found
Jessica DeSantis 's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Jessica DeSantis 's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Jessica DeSantis 's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
In situ characterization of glycans in the urothelium of donkey bladder: Evidence of secretion of sialomucins
No full textThe glycoprotein pattern was investigated by lectin histochemistry in the urothelium lining the urinary
bladder of the donkey Equus asinus. Tissue sections were stained with a panel of twelve lectins, in
combination with saponification and sialidase digestion (K-s). The urinary bladder urothelium has three
distinct layers from the basal zone to the lumen consisting of basal, intermediate and superficial cells
(umbrella cells). Cytoplasm of basal cells reacted with SNA, PNA, K-s-PNA, GSA I-B4 and Con A showing
glycans ending with Neu5Ac2,6Gal/GalNAc, Neu5AcGal1,3GalNAc, Gal and with terminal/internal
Man. The cytoplasm of umbrella cells displayed an increase of Neu5AcGal1,3GalNAc and the appearance
of Neu5AcGal1,3GalNAc, Neu5ac2,3Gal1,4GlcNAc and Neu5AcGalNAc residues (MAL II, K-s-SBA
and K-s-HPA staining). Scattered umbrella cells were characterized by glycans terminating with GalNAc
binding DBA, SBA and HPA. The mucosa forms folds with a crypt-like appearance where the urothelium
shows a different pattern of glycans. The bladder luminal surface stained with K-s-PNA, K-s-DBA,
KOH-s-SBA, and K-s-HPA displaying a coating of sialoglycoproteins belonging to O-linked glycans (typical
secretory moieties). These findings show that different glycosylation patterns exist along the donkey
bladder urothelium, and different sub-populations of umbrella cells are present secreting the sialoglycans
which constitute the protective gel layer lining the bladder
Morphology and lectin-binding sites of pyloric caeca epithelium in normal and GnRH-treated Atlantic bluefin tuna (Thunnus thynnus) Linnaeus 1758
No full textABSTRACT Mucosal epithelium of pyloric caeca was studied in normal and in GnRH-treated
Atlantic bluefin tuna Thunnus thynnus L., using morphological analysis, conventional and lectin
glycohistochemistry. The lining epithelium consisted of columnar (absorptive) cells, goblet cells
and intraepithelial leucocytes. The epithelium from normal animals was significantly taller than
GnRH-treated samples. Conventional histochemistry displayed the same staining pattern in
normal and hormone-treated specimens which showed a mixture of neutral and sulphated acidic
glycoconjugates in the luminal surface and goblet cells, and neutral glycans in apical granules of
enterocytes. Lectin histochemistry revealed a different glycoconjugate pattern in normal and
GnRH-treated tunas. In normal specimens the luminal surface expressed sialoglycoconjugates
which bound MAL II, SNA, KOH-sialidase-PNA, KOH-sialidase-SBA as well as asialoglycans
stained with HPA, SBA, GSA I-B4, LTA. N-linked glycans were highlighted by Con A and KOH-sialidase-
WGA. In GnRH-treated tunas the luminal surface did not react with SNA, SBA and LTA.
The columnar cells of normal tunas bound KOH-sialisase-PNA in the apical region, KOH-sialidase-
PNA, KOH-sialidase-DBA, HPA, SBA, KOH-sialidase-SBA and KOH-sialidase-WGA in apical
granules, GSA I-B4 and LTA in the supranuclear region. GnRH-treated specimens showed some
columnar cells that stained with KOH-sialidase-WGA in the apical granules and with GSA I-B4 in
the supranuclear region. The goblet cells of normal animals produced mucins positive to PNA,
HPA, KOH-sialidase-DBA, SBA, GSA II. The latter three binding sites lacked in GnRH-treated
tunas. The results suggest that the mucosal epithelium of Thunnus thynnus L. pyloric caeca
expresses a complex glycan pattern that is affected by GnRH-treatment
Diversity of glycoconjugate pattern in the bladder urothelium of horse and donkey revealed by lectin histochemistry
No full textThe glycoconjugates of the mucosal surface of urinary bladder act as a barrier against invasion by pathogenic microorganisms and injury from toxic substances. Furthermore, they serve as a source of soluble urinary glycoproteins, which actively modify the urine composition. Glycoconjugate pattern characterizes distinct cellular populations, cell differentiation and maturation, as well as cell morpho-functional changes. We investigated the glycan pattern in the bladder epithelium by lectin histochemistry, comparatively in the horse and donkey. Tissue fragments were fixed in 4% (w/v) neutral formalin and then embedded in paraffin wax. Sections were stained with a panel of twelve lectins, in combination with sialidase (s) digestion. The entire urothelium reacted with SNA, s-PNA, s-SBA, Con A, GSA II in horse bladder, and also with MAL II and GSA I-B4 in the donkey one. The urothelium luminal surface bound MAL II, DBA, LTA in the horse and DBA and LTA in the donkey. In addition, the horse bladder stained with PNA, SBA and GSA I-B4 in the basal and luminal regions and with UEA I in the adluminal zone. These results demonstrate remarkable inter-specific difference of the glycoprotein pattern in the bladder urothelium of two Equine species, despite their close taxonomic vicinity. It is noteworthy that glycans binding PNA and UEA I lack in the epithelium lining the donkey urinary bladder
54. Torre S. Susanna (BR). P.zza Umberto I (F. 203 II NO IGM)
No full textRinvenimento di fosse granarie in Piazza Umberto
Absorptive activities of the efferent ducts evaluated by the immunolocalization of aquaporin water channels and lectin histochemistry in adult cats
No full textUltrastructural and biochemical features of
efferent ducts (EDs) are indicative of an intense
absorptive activity towards the luminal fluid. This
function was investigated by 1) the immunohistochemical
localization of different aquaporins, integral
membrane water channels that facilitate rapid passive
movement of water, and 2) the histochemical
localization of lectins, known to have specific affinity
for glycoconjugate residues. AQP1 was mostly revealed
at the apical surface and adluminal cytoplasm of nonciliated
cells and to a minor extent in their lateral plasma
membrane, whereas it was absent in ciliated cells. Blood
vessels showed AQP1-immunoreactivity, which was
present in endothelial cells of venous vessels and
capillaries and around the muscular sheath of arteries.
AQP9 was expressed in the apical zone of ciliated and
non-ciliated cells and in the lateral cell membrane.
AQP2 and AQP5 were undetectable. Lectin
histochemistry showed that non-ciliated cells contain
glycans with terminal Neu5Aca2,3Galß1,3GalNAc,
Neu5Aca2,3Galß1,4GlcNAc, Galß1,4GlcNAc, GalNAc
(s-PNA, MAL II, RCA120, SBA reactivity) and with
internal/terminal aMan (Con A affinity) at the luminal
surface and the apical region. In addition, non-ciliated
cells expressed oligosaccharides terminating with
GalNAc and Neu5Aca2,6Gal/GalNAc (SNA reactivity)
in the luminal surface and the apical zone, respectively.
Ciliated cells revealed glycoconjugates only on cilia,
which showed terminal Neu5Aca2,3Galß1,4GlcNAc (s-
RCA120 staining) and GalNAc, as well as internal/
terminal aMan and GlcNAc (s-WGA, GSA II staining).
Data provide evidence for the involvement of different
pathways in the bulk reabsorption of water and low
molecular weight solutes by the non-ciliated cell of the
cat EDs. AQP-mediated trans-cellular route can be
hypothesized, together with fluid phase endocytosis
mediated by the glycocalix and a well-developed
endocytotic apparatus. Epithelial ciliated cells, whose
main function is the movement of luminal content, might
also participate in absorptive processes to a lesser extent
Inside Greek U.: Fraternities, Sororities, and the Pursuit of Pleasure, Power, and Prestige
No full textPopular culture portrays college Greek organizations as a training ground for malevolent young aristocrats. Films such as Animal House, Revenge of the Nerds, Old School, and Legally Blonde reinforce this stereotype, but they fail to depict the enduring influence of these organizations on their members. Inside Greek U. provides an in-depth investigation of how fraternities and sororities bolster traditional, and potentially damaging, definitions of gender and sexuality. Using evidence gathered in hundreds of focus group sessions and personal interviews, as well as his years of experience as a faculty advisor to Greek organizations, Alan D. DeSantis offers unprecedented access to the world of fraternities and sororities. DeSantis, himself once a member of a fraternity, shows the profoundly limited gender roles available to Greeks: “real men” are taught to be unemotional, sexually promiscuous, and violent; “nice girls,” to be nurturing, domestic, and pure. These rigid formulations often lead to destructive attitudes and behaviors, such as eating disorders, date rape, sexual misconduct, and homophobia. Inside Greek U. shows that the Greek experience does not end on graduation day, but that these narrow definitions of gender and sexuality impede students’ intellectual and emotional development and limit their range of choices long after graduation. Ten percent of all college students join a Greek organization, and many of the nation’s business and political leaders are former members. DeSantis acknowledges that thousands of students join Greek organizations each year in search of meaning, acceptance, friendship, and engagement, and he illuminates the pressures and challenges that contemporary college students face. Inside Greek U. demonstrates how deeply Greek organizations influence their members and suggests how, with reform the worst excesses of the system, fraternities and sororities could serve as a positive influence on individuals and campus life.
This book is unique, provocative, and troubling, and it will stimulate quite a bit of conversation. It is a snapshot of four important years in the lives of those who may one day become the business, political, and legal leaders of our nation. -- Cindy Griffin, author of Feminist Rhetorical Theories
The strengths of this work lie in the careful presentation of the students\u27 words, actions, and feelings, as well as the link DeSantis makes to the sheer presence of Greeks in today\u27s business, political, and legal systems. -- Cindy Griffin, author of Invitation to Public Speaking
This book provides a vivid description of gender templates for identity and associated behavior in the Greek System of a University. -- Peggy Reeves Sanday, author of Fraternity Gang Rape: Sex, Brotherhood, and Privi
Alan DeSantis has uncovered behavior that adds to the growing evidence of troubling flaws in the American understanding of masculinity and the concomitant injustices associated with the performance of masculinity/femininity in elitist fraternity/sorority settings. -- Peggy Reeves Sanday, author of Fraternity Gang Rape: Sex, Brotherhood, and Privi
No other book treats the Greek system as fully as Inside Greek U . It is at once critical and sympathetic, showing an unparalleled understanding of perspectives of the young men and women in the Greek system. -- Scott F. Kiesling, co-editor of Intercultural Discourse and Communication: The E
[DeSantis\u27] book is . . . a clear and mostly readable overview that winds up with surprising conclusion that fraternities and sororities aren\u27t so bad after all. -- New York Times
Many books on the subject tend to fall into sensational, expose` territory, but DeSantis\u27 takes a more thoughtful, academically critical view, examining how gender roles and behaviors in particular affect and influence young men and women. It provides practical suggestions at all levels of academia to improve Greek organizations and minimize the harm they cause. A system that weilds this much power needs greater scrutiny and understanding, and DeSantis is qualified to provide it – he possesses an insider’s knowledge and access, and as an academic, he understands how the messages cultivated and promoted by the Greek system filter into society at large, and how our culture internalizes and reinforces those messages. -- Candice Jackson, Lexington Herald-Leader
Always respectful of his participants, DeSantis produces a sympathetic work that seeks to help future members of Greek organizations to critique and avoid the often harmful gender ideals that fraternities and sororities can perpetuate. This book should be required reading for college students thinking about pledging a fraternity or a sorority. -- Library Journal
“DeSantis draws upon his 25 years of experience with Greeks and argues that reforming fraternities and sororities is both possible and necessary. He delivers and account guaranteed to raise eyebrows and spark conversations in fraternity and sorority houses, as well as administration buildings across the country.” -- Kentucky Alumni
The book was a good read. Those not familiar with Greek life or the issues presented may find the book helpful. -- David S. Williams II -- Journal of College Student Development
This is powerful information, and for lawyers who interface with the Greek system, either as university counsel or as an attorney prosecuting or defending a particular house or member, DeSantis’s book is sure to become “must reading.” But even those not so employed will find it engaging and though provoking. -- Robert M. Jarvis -- Journal of Law and Educationhttps://uknowledge.uky.edu/upk_higher_education/1016/thumbnail.jp
Levomilnacipran and vortioxetine: Review of new pharmacotherapies for major depressive disorder
No full textMajor depressive disorder (MDD) is a common psychiatric disorder with an estimated lifetime prevalence rate in the range of 13% to 16% in the United States population. Patients with MDD often have symptoms such as depressed mood, loss of interest or pleasure in usual activities, changes in eating or sleeping patterns, fatigue, difficulty concentrating and thoughts of suicide. Although many pharmacotherapy treatment options are available for MDD, antidepressants can often cause adverse effects that could affect adherence to the medication. Additionally, it is estimated that MDD is unremitting in 15% of patients and 35% can have recurrent episodes. Given the high rate of recurrence and the adverse effects associated with existing medications, new treatment options for depression are needed. Both levomilnacipran and vortioxetine are new antidepressants that were approved by the food and drug administration in 2013 for the treatment of MDD in adults. Levomilnacipran is a serotonin norepinephrine reuptake inhibitor that was effective in several short term studies and sustained efficacy and tolerability was demonstrated in a 48-wk extension study. Vortioxetine is a multi-modal antidepressant and it is thought to work via inhibition of the serotonin (5-HT) transporter, 5-HT3A, 5-HT7 and 5-HT1D antagonist, a 5-HT1B partial agonist, and a 5-HT1A agonist. Vortioxetine was effective in the treatment of MDD in both short-term trials as well as in the prevention of relapse in a 24-36 wk trial. Sustained efficacy and tolerability was demonstrated in several long-term open-label trials. Further studies comparing levomilnacipran and vortioxetine to other currently available antidepressants are needed to establish its place in therapy
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