893 research outputs found
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
No full textReal-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
Citation:
Pulikkot, S., Zhao, M., Fan, Z. Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils. J. Vis. Exp. (196), e64971, doi:10.3791/64971 (2023)
Intraoral Non-hodgkin′s Lymphoma In a HIV Patient
No full textNon-Hodgkin′s lymphoma (NHL) is one of the most common malignancies associated with HIV infection. AIDS related NHL are primarily of the B cell phenotype with histological evidence of intermediate or high grade malignancy. Treatment of AIDS related NHL remains more complicated than lymphoma in non-HIV infected population. A rare case of intra oral non-hodgkin′s lymphoma with its successful resolution is discussed in this article
A Study of Thermally Radiant Williamson Nanofluid Over an Exponentially Elongating Sheet with Chemical Reaction Via Homotopy Analysis Method
No full textAntioxidative properties of fermented poultry green bones
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Receptor localization and dynamics of murine natural killer cells at single cell level : using advanced fluorescence microscopy
No full textNatural Killer (NK) cells are immune cells and important for the defense against virally infected and malignant cells. NK cells are regulated by germline encoded activating and inhibitory receptors. Activating receptors specifically recognize ligands which are either encoded by infectious agents, or induced upon infection or cellular stress. Inhibitory receptors interact with self-ligands expressed on healthy cells, among them MHC class I. NK cells inspect the host cells by screening for alterations in activating and inhibitory ligand expression. The balance between input from activating and inhibitory receptors determines the NK cell response. NK cells undergo a process of functional maturation and acquisition of self-tolerance via sensing of the steady-state input through their receptors. This process is known as education. The cytotoxic activity of NK cells can be further increased by cytokines produced by other immune cells.The aim of this thesis was to characterize the differences in receptor dynamics and localization between NK cells based on either cytokine activation or educational status. Fluorescence based advanced microscopy techniques were used to quantitate receptor dynamics and spatial organization.In paper I, we investigated the influence of cytokine stimulation on the lateral diffusion of the inhibitory receptor Ly49A and its ligand MHC class I on NK cells within the cell membrane. The response to cytokine stimulation was heterogeneous among the NK cells. We characterized a subpopulation of NK cells with faster diffusion of both MHC class I and Ly49A. The receptor diffusion was established on primary NK cells using Fluorescence Correlation Spectroscopy. In paper II, a practical protocol for utilizing FCS on primary lymphocytes was presented. In Paper III, we showed that NKp46 and Ly49A were confined within microdomains on NK cells. The actin cytoskeleton and cholesterol composition of NK cells played important roles in initiating activating cell signaling. In Paper IV, we investigated the organization and clustering of activating and inhibitory receptors on educated and uneducated NK cells. We found that clusters of NKp46 and Ly49A were larger on uneducated NK cells. The nearest neighbour distances from activating to inhibitory receptors were not significantly different between educated and uneducated NK cells, thus the organization of inhibitory receptors in relation to the activating receptors do not seem to be of importance for the educational process.In summary, the findings in this thesis enlightens the importance of altered receptor dynamics and organization on NK cells depending on the state of activation and education. Furthermore, receptor dynamics could be an important aspect for understanding NK cell function.List of scientific papersI. Cytokines Induce Faster Membrane Diffusion of MHC Class I and the Ly49A Receptor in a Subpopulation of Natural Killer Cells. Sunitha Bagawath-Singh, Elina Staaf, Arie Jan Stoppelenburg, Thiemo Spielmann, Taku Kambayashi, Jerker Widengren and Sofia Johansson. Front Immunol. Feb 4;7:16. 2016. https://doi.org/10.3389/fimmu.2016.00016 II. Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy. Elina Staaf, Sunitha Bagawath-Singh, Sofia Johansson. J Vis Exp. 1;(120) 2017. https://doi.org/10.3791/54756 III. Educated Natural Killer Cells Exhibit a More Dynamic Movement of NKp46 and a Higher Confinement of the Ly49A Receptor. E. Staaf, P. N. Hedde, Sunitha-Bagawath Singh, J. Piguet, E. Gratton, S. Johansson. [Submitted]IV. Nano-structural Organization of Activating and Inhibitory Receptors in Murine Natural Killer cell Education. Sunitha Bagawath Singh, Jan Bergstrand, Daniel Rönnlund, Hans Blom, Jerker Widengren and Sofia Johansson. [Manuscript]</p
Obstructive sleep apnea and its management
No full textObstructive sleep apnea (OSA) is an increasingly common disorder. It is characterized by frequent episodes of airway obstruction associated with a reduced caliber of the upper airway and is vulnerable to further narrowing and collapse. Acute and repetitive effects of apnea and hypopnea include oxygen desaturation, reduction in intrathoracic pressure, excessive daytime sleepiness, impaired executive function and central nervous system arousals. The apnea-hypopnea index and respiratory distress index help quantify the severity of the condition. The condition is associated with several clinical symptoms of which daytime sleepiness is considered the cardinal symptom. Obesity is one of the major predisposing factors. Three types of apneas have been recognized - obstructive, central and mixed; OSA is the commonest. This review will cover aspects of their radiologic features, diagnosis and management
Development of a Model for the TPACK for Children with Learning Disability
No full textThis paper proposes the development of a model for the TPACK for college students with learning disability. A learning disability is a neurological disorder. Students with learning disabilities are smart as or smarter than their peers. But they may have difficulty in reading, writing, spelling, reasoning, recalling and or organizing information if left to figure things out by themselves or if taught in conventional ways The TPACK framework was derived from the conceptions of Shulman 1986 , who placed importance on the complex inter relationships between the teacher's knowledge about pedagogy, content, and the different ways to characterize subject matter. The broad conception of TPACK is the knowledge regarding the interactions between Content Knowledge CK , Pedagogy Knowledge PK , and Technology Content TK in teaching through the use of technology. Koehler and Mishra emphasized the importance of domains-Pedagogical Content Knowledge PCK , Technological Content Knowledge TCK , Technological Pedagogical Knowledge TPK and TPACK-as a way to see the interaction among the three main domains CK, PK and TK . This paper aims to available TPACK for college students with learning disability and facilitate their learning process in the classroom. Thus this paper tries to answer the following questions. What is meant by learning disability What is meant by TPACK Are there any TPACK available for college students with learning disability A suggestive model of TPACK for college students with learning disability. So, in this paper a TPACK model is presented for college students with learning disability in the higher education. Dr. K. S. Sajan | Sunitha M S "Development of a Model for the TPACK for Children with Learning Disability" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: https://www.ijtsrd.com/papers/ijtsrd8389.pd
Liver transplantation and recurrence of Hepatitis C
No full textIl capitolo tratta delle indicazioni al trapianto di fegato per epatite C e della recidiva della patologia dopo il trapianto stesso
Sunitha Krishnan i jej walka z seksualnym niewolnictwem kobiet w Indiach
No full textThis article introduces the silhouette of Hindu activist Sunitha Krishan, has been strongly committed to the fight against sexual slavery in India for 20 years. It presents the circumstances of the fact that she started her business, initiatives which was undertaken by her and what was the results of her activities. In summary form the article presents stories that Krishnan has collected by seeing with victims – women and children. The author also essays the presentation of Hindu models of femininity and she demonstrates their influence on the current situation of women. This text is largery based on online sources, articles published on the information portals, interviews with Krishnan and record during the conference with her participation.Niniejszy artykuł przybliża sylwetkę hinduskiej aktywistki Sunithy Krishan, która od 20 lat jest silnie zaangażowana w walkę z seksualnym niewolnictwem w Indiach. Przedstawia okoliczności, w jakich doszło do rozpoczęcia jej działalności, inicjatywy przez nią podejmowane oraz rezultaty działań. W skrótowej formie prezentuje opowieści, które zebrała Krishnan spotykając się z pokrzywdzonymi kobietami i dziećmi. Autorka podejmuje również próbę przedstawienia hinduskich modeli kobiecości i wykazania jaki mają one wpływ na obecną sytuację kobiet. Tekst oparty jest w dużej mierze na źródłach internetowych, artykułach publikowanych na portalach informacyjnych, wywiadach przeprowadzonych z Krishnan oraz zapisie konferencji z jej udziałem.Udostępnienie publikacji Wydawnictwa Uniwersytetu Łódzkiego finansowane w ramach projektu „Doskonałość naukowa kluczem do doskonałości kształcenia”. Projekt realizowany jest ze środków Europejskiego Funduszu Społecznego w ramach Programu Operacyjnego Wiedza Edukacja Rozwój; nr umowy: POWER.03.05.00-00-Z092/17-00
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