36 research outputs found

    Lamprima Latreille 1804

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    Lamprima Latreille, 1804 Lamprima Latreille, 1804a: 150. Type species. Lethrus aeneus Fabricius, 1792, by monotypy. Neolamprima Gestro, 1875: 997; Nagel 1922: 16 (synonymy). Type species. Neolamprima adolphinae Gestro, 1875, by monotypy. Description. Length. Male 13–60 mm (usually 20–45 mm) including mandibles, mandibles 10–38% of overall length (Figs 1–14); female 13–27 mm including mandibles, mandibles 5–9% of overall length (Figs 15–20). Dorsal surface without scales or visible setae; venter setose, setae simple. Head. Male without dorsal tubercles on head, anterior of head concave or truncate (Figs 21–32); genae welldeveloped anterior to eyes but not laterally expanded, at most slightly anteriorly angulate in males; female head not narrowed compared with male; temples short but slightly angulate, shallowly grooved or notched to accommodate anterior angles of pronotum; head deeply inserted into pronotum which almost reaches eyes (Figs 33–44); eyes undivided, reniform, with shallowly concave anterior and posterior margins, the anteroventral margin sharp and linear, forming the outer edge of the antennal groove; antennae not geniculate, with 10 antennomeres, with 3 antennomere club, the club antennomeres entirely densely setose and often closely appressed; antennomere 7 cupuliform, with thin lateral lobe and 6 slightly asymmetric (male) or with thin lobe (female); male mandibles as long as or longer than head and usually densely internally setose in male (not L. imberbis); male mandible without basal dorsal tooth; each female mandible with only one dorsal cusp, with large strongly incurved basal ventral tooth, overlapping at tips; penultimate labial palpomere angulate on inner margin in male, not angulate in female; labium broad, approximately one third width of head; mentum solid and punctate, apex truncate; pregula flat; lateroventral grooves present between eyes and sides of buccal cavity for retention of scape. Thorax. Pronotum (Figs 1–44) convex with angulate (most males) to rounded sides, broadest slightly posterior to middle, without prominent anterior or posterior angles; anterior of pronotum broadly margined; middle of prosternal process concealed between procoxae, apex flat. Scutellum almost equilateral triangular. Elytra nonstriate but may have irregular, shallow grooves, with scattered shallow simple concave punctures, surface of interspaces smooth and shiny to dull and wrinkled but always with finely microreticulate microsculpture, usually slightly granulose in males (Figs 54–57). Elytral humeri not spined. Elytral epipleurae hidden from above, transversely strigose. Hindwings fully developed. Anterior field of mesoscutum strongly and closely punctate; scutellum semicircular to heart shaped; mesanepisternum with sparse, large punctures; posterior half mesepimeron, visible parts of metanepisternum, metepimeron and sides of metaventrite with dense, small punctures (partly coalescent, interspaces less than diameters) and setae; mesoventral process almost parallel-sided to junction with metaventrite, abruptly elevated anterior to this (Figs 58–61); metanepisternum with elevated lobe at anterior angle locking into small depression on elytral epipleuron. Profemur without anterior ridge; male protibia (Figs 62–75) with expanded blade- or fan-like flat spur, except L. imberbis with narrowly elongate, triangular, curved spur; female protibial spur narrowly elongate triangular; male protibia without secondary teeth between major teeth; female protibia without subsidiary teeth between large teeth on outer edge; male metatibia without setose excavation on inner edge, usually with spines on outer edge; tarsal empodium prominent, with paired divergent thin tufts of apical setae. Abdomen. Ventrites not laterally ridged and without basal transverse grooves. Male genitalia (Figs 76–88; unique male of L. imberbis undissected). Phallobase fusiform or spindle-shaped and uniformly sclerotised, with the apical margins rounded laterally and with V-shaped excavations dorsally (shallower) and ventrally (deeper); apical half of dorsal surface with at least a few small raised spicules, which may form irregular oblique ridges. Parameres about 2/5 length phallobase, symmetrical, with preapical setal tuft on ventral surface and incurved pointed tip; ventral inner edge of parameres soft, irregularly ridged, with the surface either inflated towards the penis or collapsed laterally, depending on preservation of the specimen; penis symmetrical, in two visible parts: basal 2/3 darkly sclerotised, rigid and narrowly conical between parameres, obliquely ridged at base; apical third thinly sclerotised and usually translucent, as a thin straight cylinder; endophallus, if everted, of similar width to apex of penis but soft and flexible, apically contracting to long, thin flagellum. Female genitalia (Figs 89–94; L. imberbis unknown). Tergite IX with acute to broadly rounded translucent apex; pleurite IX and sternite IX well developed as elongate sclerites; hemisternites flat and apical halves elongate rectangular with truncate apices, gonostyli flat, inverted-trapezoidal in shape, inserted on middle of membranous apex of hemisternites; spermathecal duct coiled and convoluted, spermatheca sclerotised, variable in shape from tear-drop to hook; spermathecal gland smaller than spermatheca, glandular duct longer than spermatheca. Larval diagnosis. The following diagnosis is based on published descriptions of L. aurata larvae (Alderson 1975 [as L. varians]; Lawrence 1981). The larva is similar to the lamprimine Phalacrognathus W.J. Macleay, 1885 (Wood et al. 1996). Head: antenna with 3 antennomeres; second antennomere without setae or sensory spots, apex slightly produced beyond base of third antennomere, the latter elongate but much smaller than second. Left mandible without incisor teeth between 2–3 apical teeth and mola; epipharynx trapezoidal, with dense, long setae on apical margin and dense, short setae on sides, apex of median area with transverse row of 6 short, blunt pegs and row of 4 circular sensilla proximal to this, epitorma absent. Legs without distal claw, last 3 segments short and broad and densely setose; tibiotarsus reduced to an ovate lobe, length equal to width at base; mesocoxal stridulatory file (pars stridens) with a single line of about 50 quadrate to slightly transverse dense ridges, granulose towards base, and placed in a field of minute granules; metatrochanteral stridulatory file (plectrum) a single line of 45–60 closely placed transverse ridges or granules, increasing in size from base to apex. Anal area of abdomen with two adjacent pear-shaped and minutely but densely setose lobes, without ovate pads, their bases subtending about 90° in ventral view, separated from 10th segment by a dorsal lobe, which is triangular, glabrous and unsculptured; 10th abdominal segment not foreshortened dorsally, ventral apex triangularly excavate, raster confined to apical quarter, consisting of dense, minute, inwardly-directed setae. Ecology and natural history. The following notes mostly concern the common and widespread species L. aurata (Fearn 1996, 2015, 2016) and the Lord Howe endemic L. insularis (Reid 2004). Lamprima adults are commonly diurnal and are often found on flowers, where they may mate. Adult males snip off the apical shoots of living plant material to provide sap flows. They have been recorded feeding on many genera, both native and exotic, listed by family as follows: Asparagaceae: Lomandra; Asteraceae: Ozothamnus; Casuarinaceae: Casuarina; Fabaceae: Acacia, Virgilia; Malvaceae: Lavatera; Myrtaceae: Eucalyptus, Leptospermum, Melaleuca; Pinaceae: Pinus; Proteaceae: Banksia; Rhamnaceae: Alphitonia; Rosaceae: Photinia, Prunus; Salicaceae: Populus (Fearn 1996, 2015; Hangay & de Keyzer 2017). Feeding by the males of Lamprima with elongated mandibles (most L. adolphinae and some L. aurata) has not been described. Female mandibles are apparently non-functional, therefore females fly to the male feeding sites to lap up sap released by males. Feeding sites are used for male-male aggression and for copulation. Fearn (1996) noticed a 3: 1 male to female sex ratio in the field, which is corroborated by the male biased material of most species in collections. Olliff (1889) noted that male L. insularis were much more common than females. In contrast, the material available for L. insularis has a roughly 1:1 sex ratio, but were mostly collected by breaking open logs, so does not represent typical field activity of the species. Adults will also feed on soft fruits (Hangay & de Keyzer 2017). Oviposition is usually underground, the females tunnelling into soil around partly buried decaying wood. There is a great variety of larval host genera, including exotics: Araucariaceae: Araucaria; Arecaceae: Howea; Casuarinaceae: Allocasuarina, Casuarina; Celastraceae: Elaeodendron; Fabaceae: Acacia; Lauraceae: Cryptocarya; Myrtaceae: Eucalyptus, Syzygium; Oleaceae: Olea; Salicaceae: Salix (Fearn 1996; Reid 2004; Hangay & de Keyzer 2017). Lamprima aurata larvae are usually in decaying roots and buried timber in Tasmania (Fearn 1996) but they prefer standing timber in northern Queensland rainforest (Wood et al. 1996). Lamprima insularis larvae usually inhabit fallen timber on or above ground level in the subtropical rainforests of Lord Howe Island (Reid 2004). In wetter areas Lamprima larvae may be better able to survive above ground, or less able to survive below ground, but there may be a trade-off between humidity and temperature, as L. aurata avoids cool temperate rainforests in Tasmania (Fearn 1996). In logs and stumps the larvae generally bore upwards. Pupation is in a chamber, usually just beneath the wood surface but sometimes in adjacent soil (Fearn 1996). In Tasmania the entire life cycle is at least three years but it may be 1–2 years in Queensland (Hangay & de Keyzer 2017). In captivity, the life cycle of L. adolphinae is 9–14 months (Levet 2016). There are numerous photographs and several videos of Lamprima species on the Internet (for example: Anonymous 2017a), showing: different colour varieties, mating, fighting between males, feeding, rearing methods, larvae and pupae. In copulation and precopulation the protibial spurs of the males have little function. They may scrape lightly over the pronotum of the female as the prothoracic legs are moved backwards and forwards, but this activity seems erratic and brief. In male-to-male combat, each male uses its mandibles to try to embrace the mandibles of the other, so longer mandibles provide a wider net for the embrace. Once one male has enclosed and squeezed together the mandibles of its rival, it shakes the whole animal quickly to one side to unbalance it, then abruptly to the other side, letting go at the end of this second swing. The rival can be flung a few centimetres (see video by Kan 2016). The elongate mandibles of L. adolphinae allow males to grab wayward appendages of rivals rather than gripping the whole head. In fights, the protibial spurs may be used as braces against the substrate and this activity might be their primary function. The international pet trade is heavily involved in rearing Lamprima species, with goals including production of enlarged mandibles and unusual colour varieties. This may extend the range of variation for each species given here, which is based on field-collected specimens. Lamprima is widespread in Australia (Fig. 95), occupying almost the entire eastern edge of the continent from Cooktown in northern Queensland to Tasmania and most of the south coast from Mallacoota west to Perth, with an 850 km gap at the Nullarbor Plain. Lamprima occurs up to 400 km inland on the mainland. Lamprima also occurs on two oceanic islands, Norfolk and Lord Howe and a single species is widespread on mainland New Guinea (Fig. 95). In New Guinea it occurs up to 2800 m. Conservation. Conservation status and threats are discussed under each species. In general Lamprima species are extremely popular with stag beetle collectors and most species are being, or have been, reared in commercial quantities in eastern Asia and probably Europe and North America. One species, L. imberbis, is of considerable concern as it has not been collected for 100 years. Comparison with other genera of Lampriminae. There are four other genera of Lampriminae, all monotypic. The New Zealand genus Dendroblax White, 1846, is unmistakably different from Lamprima, with a dynastine-shaped body, densely punctate, non-metallic, reddish-brown upper surface, venter with long setae and minimal sexual dimorphism (Holloway 2007). The Australian endemic Homolamprima W.J. Macleay, 1885, is relatively easily distinguished from Lamprima by: mesometaventrite junction anteriorly bilobed; apex prosternal process elevated; male protibia with large narrow spines. Homolamprima is also much flatter than any Lamprima species (Macleay 1885b). The South American genus Streptocerus Fairmaire, 1850, is similar to Homolamprima but differs from it and all other Lampriminae by the antennal club having four antennomeres (Paulsen 2010). Lamprima is morphologically most similar to the northern Australian endemic Phalacrognathus Macleay, 1885, although this is not supported by an analysis of four gene regions (Kim & Farrell 2015). Most species of Lamprima can easily be distinguished from Phalacrognathus by male protibia with spur expanded as a flat blade and female protibia without subsidiary teeth between large teeth on outer margin. In Phalacrognathus, the male protibia has a simple spur (as in female) and the female protibia has small subsidiary teeth present between the large teeth on the outer margin. However, Lamprima imberbis, with unknown female, is unusual in Lamprima for its male mandibles lacking internal setae, male protibiae with narrow spurs and without a setal tuft and elytra broadly explanate. It shares these characters with Phalacrognathus, but differs from that genus by: genae prominent anterior to eyes; eyes anteriorly concave; antennomere 7 with flat lateral lobe; temples prominent and notched to accommodate anterior angles of pronotum; male mandible without basal dorsal tooth; anterior of pronotum broadly margined; middle of prosternal process concealed by procoxae; sides of male elytra not crenulate; male protibia without secondary teeth between major teeth; protibial spur on a lobe. All of these characters are common to other male Lamprima and justify placement of L. imberbis in Lamprima. Included species. The most recent peer-reviewed checklist of Lamprima species (Moore & Cassis 1992) lists seven in Australia (Table 1), of which Lamprima insularis is unique to Lord Howe Island and Lamprima aenea is unique to Norfolk Island. One non-Australian species of Lamprima is known, from New Guinea: L. adolphinae, as catalogued by Benesh (1960). The mainland Australian species listed by Moore & Cassis (1992) are L. aurata, L. imberbis, L. latreillii, L. micardi and L. varians. Lamprima micardi is supposedly endemic to Western Australia and L. varians was described from South Australia. The other species, L. aurata, L. imberbis and L. latreillii, were described from the eastern coastal region of Australia, from northern Queensland to Tasmania. Lamprima Author Date Type Hope in Parry Harold Macleay Boileau Nagel 1930 Benesh Moore & Krajcik This work species rank locality Westwood 1864, 1868 1885a 1913 1960 Cassis 2001 2017 name 1845 1870 1992 L. adolphinae Gestro 1875 NG - - - L. - L. L. - L. L. adolphinae adolphinae adolphinae adolphinae adolphinae L. aenea Fabricius 1792 NI L. aenea L. aenea L. aenea L. aenea L. aenea L. aenea L. aenea L. aenea L. aenea L. aenea L. aenea Fabricius 1802 NI L. L. aenea L. aenea - - - - L. aurata - L. aenea & sensu schreibersi L. aurata Schreibers L. aenea Fabricius 1805 NI & - L. aurata - L. aurata - - L. aurata L. aurata L. aurata L. aenea & sensu NH L. aurata Donovan L. aenea Boisduval 1835 NH - L. L. latreillii L. latreillii - - L. latreillii - L. latreillii L. aurata latreillii L. amplicollis Thomson 1862 SQ - L. L. latreillii L. latreillii - - L. latreillii L. latreillii L. latreillii L. aurata latreillii L. fulgida Thomson 1862 NH - L. - - - - L. latreillii - L. latreillii L. aurata splendens ……continued on the next page Lamprima Author Date Type Hope in Parry Harold Macleay Boileau Nagel 1930 Benesh Moore & Krajcik This work species rank locality Westwood 1864, 1868 1885a 1913 1960 Cassis 2001 2017 name 1845 1870 1992 L. imberbis Carter 1926 NSW - - - - - L. imberbis L. L. L. L. imberbis imberbis imberbis imberbis L. insularis Hope 1845 WA L. micardi - nomen - L. micardi - L. micardi - L. micardi nomen nudum nudum L. insularis W.J. 1885a LHI - - - L. insularis - L. insularis L. L. L. L. Macleay insularis insularis insularis insularis L. insularis Boileau 1913 not - - - - L. micardi - - - - L. aurata given L. krefftii W.J. 1871 CQ - - - L. krefftii - L. latreillii? L. latreillii L. latreillii L. latreillii L. aurata MacLeay L. latreillii W.S. 1819 NSW L. latreillii L. L. latreillii L. latreillii L. latreillii L. latreillii L. latreillii L. latreillii L. latreillii L. aurata Macleay latreillii L. lulua Kriesche 1940 NG - - - - - - adolphinae - L. L. adolphinae adolphinae L. W.J. 1885a NQ - - - L. - L. latreillii L. latreillii L. latreillii L. latreillii L. aurata mandibularis Macleay mandibulari s L. mariae Lea 1910 Tas - - - - - L. aurata L. aurata L. aurata L. aurata L. aurata L. micardi Reiche 1841 WA L. micardi L. L. micardi L. micardi L. micardi L. micardi L. micardi L. micardi L. micardi L. aurata micardi L. minima W.J. 1885a SA - - - L. minima - L. varians L. varians L. varians L. micardi L. aurata Macleay ……continued on the next page Lamprima Author Date Type Hope in Parry Harold Macleay Boileau Nagel 1930 Benesh Moore & Krajcik This work species rank locality Westwood 1864, 1868 1885a 1913 1960 Cassis 2001 2017 name 1845 1870 1992 L. pygmaea W.S. 1819 AUS - L. L. latreillii L. latreillii - - L. latreillii L. latreillii L. latreillii L. aurata MacLeay latreillii L. rutilans Erichson 1842 Tas - L. aurata L. rutilans L. rutilans - L. aurata L. aurata L. aurata L. aurata L. aurata L. viridis Erichson 1842 not - L. aenea L. aenea ? - L. aurata L. aurata L. aenea L. aenea L. aurata given There are good male characters for diagnosing L. aenea, L. adolphinae, L. imberbis and L. insularis. There remain the Lamprima species, excluding L. imberbis, described from mainland Australia and Tasmania. It has already been noted that Matthews (1984) treated L. varians and L. aurata as one species in South Australia (L. aurata) and Moore (1984, 1986) suggested that L. aurata and L. latreillii were variants (“overlapping subspecies”) of one species in southeastern Australia, even though these were originally separated by dorsal punctation and structure of thoracic ventrites (Macleay 1885a). Lamprima micardi in Western Australia and L. varians from South Australia were originally distinguished from eastern Australian Lamprima by their narrower male protibial spurs (Reiche 1841; Burmeister 1847) but the presence of intermediates blurs their distinction. We have examined more than 400 specimens of Lamprima from throughout southern and eastern Australia and conclude that these represent just one species, L. aurata.Published as part of Reid, Chris A. M., Smith, Kindi & Beatson, Max, 2018, Revision of the genus Lamprima Latreille, 1804 (Coleoptera: Lucanidae), pp. 151-202 in Zootaxa 4446 (2) on pages 154-160, DOI: 10.11646/zootaxa.4446.2.1, http://zenodo.org/record/145425

    Cool Little Kids translational trial to prevent internalising: two‐year outcomes and prediction of parent engagement

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    BACKGROUND: The aim was to determine outcomes in the first year of school of a population-delivered parenting program to prevent internalising problems in temperamentally inhibited preschool children and predictors of engagement in parenting groups. METHOD: Design: Randomised controlled trial. SETTING: 307 preschool services across eight socio-economically diverse government areas in Melbourne, Australia. PARTICIPANTS: 545 parents of inhibited 4-year-old children; 469 (86%) retained at two-year follow-up. INTERVENTION: Cool Little Kids program. Primary outcomes were child internalising symptoms and anxiety disorders. Secondary outcomes were parenting, parent well-being and engagement. Trial registration ISRCTN30996662 http://www.isrctn.com/ISRCTN30996662. RESULTS: In the first year of school (M (SD) age 6.7 (0.4) years), child anxiety symptoms were reduced in the intervention versus control arm (PAS-R M (SD): total 36.2 (17.2) versus 39.4 (18.5); adjusted difference -3.26, 95% CI -6.46 to -0.05, p = .047; specific fears 9.1 (6.2) versus 10.7 (6.8), adjusted difference -1.53; 95% CI -2.69 to -0.38, p = .009). However, there was little difference in broader child internalising (CMFWQ M (SD): 2.2 (0.5) versus 2.3 (0.6); adjusted difference -0.03, 95% CI -0.13 to 0.06, p = .489) or anxiety disorders (37.6% vs. 42.6%; adjusted OR 0.79, 95% CI 0.53 to 1.18, p = .242). Lower income, younger mothers, less educated and more culturally diverse fathers engaged less with the intervention. Continued skills practice was less frequent for parents of girls and in advantaged neighbourhoods. CONCLUSIONS: There were population effects of Cool Little Kids in the first year of school for anxiety symptoms but not disorders. Considering motivation techniques to engage subgroups of families would be helpful in translation research

    Bilingual legislation: the consistency between two languages of law

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    This dissertation gives an analysis of the importance of consistency as an essential element in achieving quality in bilingual legislation. It explains the concept of bilingual legislation and the process of bilingual legislative drafting, making references to jurisdictions which deals with bilingual legislation with background on the constitutional and legislative framework. The author identifes the possible solutions that have been undertaken by jurisdictions associated with bilingual legislation in order to achieve consistency between the two language versions of the same law

    Disabled Māori and disability support options

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    The goal of this research project was to provide information to enhance the development and implementation of an effective model of disability support service provision to Maori with disabilities. This was done by identifying and documenting the needs expressed by disabled Maori and their carers living in the Midland area, and by considering the experiences and observations of a key informant group. From our reading of the literature and the feedback we received from participants, a proposed model of disability support service provision has been proposed. In five sections, this report begins by defining disability, and also by defining the act of caring for the disabled. Focussing on the Maori experience, obstacles, access to existing support services, cultural barriers, and health policies are discussed. In the second chapter, the research methodology is described, along with how the information was gathered. Following this is the third section which presents the opinions and reflections of Maori with disabilities. It concludes strategically with a view of the ideal community, suggesting possible resolutions, by exposing current flaws and inadequacies. Chapter four records the views and perspectives of key informant/whanau carers who participated in the project. They offer an insightful account of the often unrecognised side of the disability experience. Considering the preceding discussions, the final section develops a model of service provision for Maori with disabilities, and proposes this for effective service delivery

    Barriers and facilitators to data-based decision making in Australian early childhood education and care: A qualitative study

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    Early childhood education and care (ECEC) provides a critical platform for children's development in the early years. To be of benefit to all children, ECEC must deliver on three key areas: availability (quantity), quality and participation (reach and dose). Lead indicators can be derived and applied to help service providers drive equitable systems change. Yet there is little evidence that the ECEC sector is able to utilise lead indicators and data for this purpose. In this study, we used the Theoretical Domains Framework (TDF) and the Capability, Opportunity, and Motivation model of Behaviour (COM-B) to explore barriers and facilitators to data-utilisation behaviours (collecting, reporting, and using lead indicator data) by ECEC service providers. Semi-structured interviews with ECEC service staff and managers were conducted between May and November 2022. Barriers spanned issues of capability (knowledge), opportunity (data systems and processes, data quality, data availability, resources) and motivation (data culture, trust, pressure). Important facilitators related to capability (ability to use data systems, cognitive skills and interpersonal skills), opportunity (environmental context and resources or social influences) and motivation (reinforcement and professional/social role and identity). Multipronged strategies, including introducing practical data systems, ensuring adequate staff resources, providing additional funding and data-use training, and developing organisational data culture that promotes trust could increase lead indicator data use in ECEC. These findings can guide service improvement efforts and help to ensure that children and families can access high-quality services when and where they need them

    Translational delivery of Cool Little Kids to prevent child internalising problems: Randomised controlled trial

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    Objective: To determine whether a population-delivered parenting programme assists in preventing internalising problems at school entry for preschool children at-risk with temperamental inhibition. Methods: Design: a randomised controlled trial was used. Setting: the setting was 307 preschool services across eight socioeconomically diverse government areas in Melbourne, Australia. Participants: a total of 545 parents of inhibited 4-year-old children: 498 retained at 1-year follow up. Early intervention: Cool Little Kids parenting group programme was implemented. Primary outcomes: the primary outcomes were child DSM-IV anxiety disorders (assessor blind) and internalising problems. Secondary outcomes: the secondary outcomes were parenting practices and parent mental health. Results: At 1-year follow up (mean (standard deviation) age = 5.8 (0.4) years), there was little difference in anxiety disorders between the intervention and control arms (44.2% vs 50.2%; adjusted odds ratio = 0.86, 95% confidence interval = [0.60, 1.25], p = 0.427). Internalising problems were reduced in the intervention arm (Strengths and Difficulties Questionnaire: abnormal – 24.2% vs 33.0%; adjusted odds ratio = 0.56, 95% confidence interval = [0.35, 0.89], p = 0.014; symptoms – mean (standard deviation) = 2.5 (2.0) vs 2.9 (2.2); adjusted mean difference = –0.47, 95% confidence interval = [–0.81, –0.13], p = 0.006). Parents’ participation in the intervention was modest (29.4% attended most groups, 20.5% used skills most of the time during the year). A priori interaction tests suggested that for children with anxious parents, the intervention reduced anxiety disorders and internalising symptoms after 1 year. Conclusion: Offering Cool Little Kids across the population for inhibited preschoolers does not impact population outcomes after 1 year. Effects may be emerging for inhibited children at highest risk with parent anxiety. Trial outcomes will continue into mid-childhood. </jats:sec

    Am I doing the right thing? Plunket nurses' experience in making decisions to report suspected child abuse and neglect

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    Suspected child abuse and neglect is not a new phenomenon in community nursing. Child abuse and/or neglect is prevalent globally and is a major community concern. Plunket Nurses have a primary responsibility to protect the health and well being of the women and children with whom they come into contact. Detecting suspected child abuse and/or neglect and making decisions to report to Child, Youth and Family, New Zealand’s Statutory Agency, is difficult. There are professional, legal, ethical and moral complexities in this work. Boyne (2003) states that there has not been enough research about what it is like to work with and manage risks in child protection work. This study set out to report these experiences in view of understanding them and finding possible gaps in literature, policy, and education. Hermeneutic phenomenology was the methodology thought most appropriate to study the experiences of Plunket Nurses making decisions to report suspected child abuse and/or neglect in uncertain situations. A purposeful sample was selected to ensure participants were able to provide rich data that was captured in semi-structured, face to face and telephone recorded interviews. Data analysis was guided by the framework developed by van Manen (1990) to formulate meaning from participant experiences. Four major themes developed. Ethical considerations were extensively explored due to the sensitive nature of the study. Management of possible ethical situation have been described, with a planned approach to an ongoing consent process throughout the data collection. The results have identified gaps in the literature, Plunket policy and the educational needs of Plunket Nurses. Opportunities for future research are suggested

    Recombination is a key driver of genomic and phenotypic diversity in a Pseudomonas aeruginosa population during cystic fibrosis infection

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    The Cystic Fibrosis (CF) lung harbors a complex, polymicrobial ecosystem, in which Pseudomonas aeruginosa is capable of sustaining chronic infections, which are highly resistant to multiple antibiotics. Here, we investigate the phenotypic and genotypic diversity of 44 morphologically identical P. aeruginosa isolates taken from a single CF patient sputum sample. Comprehensive phenotypic analysis of isolates revealed large variances and trade-offs in growth, virulence factors and quorum sensing (QS) signals. Whole genome analysis of 22 isolates revealed high levels of intra-isolate diversity ranging from 5 to 64 SNPs and that recombination and not spontaneous mutation was the dominant driver of diversity in this population. Furthermore, phenotypic differences between isolates were not linked to mutations in known genes but were statistically associated with distinct recombination events. We also assessed antibiotic susceptibility of all isolates. Resistance to antibiotics significantly increased when multiple isolates were mixed together. Our results highlight the significant role of recombination in generating phenotypic and genetic diversification during in vivo chronic CF infection. We also discuss (i) how these findings could influence how patient-to-patient transmission studies are performed using whole genome sequencing, and (ii) the need to refine antibiotic susceptibility testing in sputum samples taken from patients with CF
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