14 research outputs found
Biofunctionalization of magnetite nanoparticles with stevioside: effect on the size and thermal behaviour for use in hyperthermia applications
No full textControlling the magnetic properties of a nanoparticle efficiently via its particle size to achieve optimized heat under alternating magnetic field is the central point for magnetic hyperthermia-mediated cancer therapy (MHCT). Here, we have shown the successful use of stevioside (a natural plant-based glycoside) as a promising biosurfactant to control the magnetic properties of Fe3O4 nanoparticles by controlling the particle size. The biocompatibility and cellular uptake efficiency by rat C6 glioma cells and calorimetric magnetic hyperthermia profile of the nanoparticles were further examined. Our finding suggests superior properties of stevioside-coated magnetite nanoparticles in comparison to polysorbate-80 and oleic acid coated nanomagnets as far as particle size reduction, biocompatibility, hyperthermic effect, and cellular uptake by the glioblastoma cancer cells are concerned. The stevioside-coated nanomagnets exhibiting the maximum temperature rise were further investigated as heating agents in in vitro magnetic hyperthermia experiments (405 kHz, 168 Oe), showing their efficacy to induce cell death of rat C6 glioma cells after 30 min at a target temperature T = 43 °C.</p
Manganese-Doped Magnetic Nanoclusters for Hyperthermia and Photothermal Glioblastoma Therapy
No full textThe development of
nanostructures with complementary functionalities
has emerged as a prerequisite for more efficient preclinical nanoparticle-mediated
thermo-therapeutic research. Here, we report the bimodal application
of manganese doped-iron oxide nanoclusters for photothermal and magnetic
hyperthermia-mediated glioblastoma therapy. Besides the combinatorial
effect, we have also explored the comparative effects of the single-mode
therapies when seldom used in terms of cell viability, oxidative stress
production, reduction in mitochondrial membrane potential, cytoskeletal
damage, and morphological alterations. In all aspects, exposure to
magnetic hyperthermia was shown to have a higher therapeutic effect
than the photothermal therapy when used alone. However, it is ultimately
the consequence of bimodal therapy application that results in significant
death of rat glioma C6 cells. Excitation of cells with a laser was
observed to create oxidative stress in the cellular environment which
enhanced the efficiency of magnetic hyperthermia, resulting in a remarkable
anticancer effect mediated by ROS-dependent apoptosis via the mitochondrial
pathway
Fig. 7 in A new deepwater species of Calliopiidae, Halirages helgae (Crustacea, Amphipoda), with a synoptic table to Halirages species from the northeast Atlantic
No full textFig. 7. Halirages helgae sp. nov., holotype, ♀, 10 mm long. A. Uropod 1. B. Uropod 2. C. Telson. D. Epimeron 2–3.Published as part of Ringvold, Halldis & Tandberg, Anne Helene Solberg, 2014, A new deepwater species of Calliopiidae, Halirages helgae (Crustacea, Amphipoda), with a synoptic table to Halirages species from the northeast Atlantic, pp. 1-13 in European Journal of Taxonomy 98 on page 9, DOI: 10.5852/ejt.2014.98, http://zenodo.org/record/383895
MOESM1 of Association of trans fatty acids with lipids and other cardiovascular risk factors in an Indian industrial population
No full textAdditional file 1: Table S1. Percent content of fatty acids in serum
UDAY: a comprehensive diabetes and hypertension prevention and management program in India
No full textINTRODUCTION: Diabetes and hypertension are two leading non-communicable conditions, which are suboptimally managed in India. Thus, innovative comprehensive approaches that can concomitantly improve their detection, prevention and control are warranted. METHODS AND ANALYSIS: UDAY, a 5-year initiative, aims to reduce the risk of diabetes and hypertension and improve management by implementing a comprehensive intervention programme in the two selected study sites, Sonipat and Visakhapatnam (Vizag). It has a pre-post evaluation design with representative cross-sectional surveys before and after intervention. Within these two sites, urban and rural subsites each with a total population of approximately 100 000 people each were selected and a baseline and postintervention assessment was conducted deploying five surveys [among general population (including body measurements or biosamples), patients, healthcare providers including physicians and pharmacists, health facilities], which will determine the knowledge levels about diabetes and hypertension, the proportion treated and controlled; the patient knowledge and self-management skills; healthcare providers' management practices; the level of access and barriers to obtaining care.The interventions will include: tailored health promotion for improving public knowledge; screening of adults aged ≥ 30 years for identifying those at high risk of diabetes and/or hypertension for linkage to the healthcare system; patient education using technology enabled community health workers, geographic information system (GIS) based mapping of the communities, healthcare provider training on management guidelines, community based diabetes registry and; advocacy to improve access to healthcare. The baseline surveys have been completed, the study areas mapped using GIS and the interventions are being implemented. UDAY is expected to increase over baseline the levels of: public knowledge about diabetes and hypertension; those treated and controlled; patient self-management skills; the use of guideline based management by providers and; access to healthcare, leading to improved health outcomes and inform development of a India relevant chronic care model. ETHICS AND DISSEMINATION: Ethical clearance for conduct of the study was obtained from the Institutional Ethics Committee (IEC) of the Public Health Foundation of India. The findings will be targeted primarily at public health policymakers and advocates, but will be disseminated widely through other mechanisms including conference presentations and peer-reviewed publications, as well as to the participating communities
Table1_Evaluation of genetic variants related to lipid levels among the North Indian population.DOCX
No full textBackground: A heavy burden of cardiometabolic conditions on low- and middle-income countries like India that are rapidly undergoing urbanization remains unaddressed. Indians are known to have high levels of triglycerides and low levels of HDL-C along with moderately higher levels of LDL-C. The genome-wide findings from Western populations need to be validated in an Indian context for a better understanding of the underlying etiology of dyslipidemia in India.Objective: We aim to validate 12 genetic variants associated with lipid levels among rural and urban Indian populations and derive unweighted and weighted genetic risk scores (uGRS and wGRS) for lipid levels among the Indian population.Methods: Assuming an additive model of inheritance, linear regression models adjusted for all the possible covariates were run to examine the association between 12 genetic variants and total cholesterol, triglycerides, HDL-C, LDL-C, and VLDL-C among 2,117 rural and urban Indian participants. The combined effect of validated loci was estimated by allelic risk scores, unweighted and weighted by their effect sizes.Results: The wGRS for triglycerides and VLDL-C was derived based on five associated variants (rs174546 at FADS1, rs17482753 at LPL, rs2293889 at TRPS1, rs4148005 at ABCA8, and rs4420638 at APOC1), which was associated with 36.31 mg/dL of elevated triglyceride and VLDL-C levels (β = 0.95, SE = 0.16, p Conclusion: We confirm the role of eight genome-wide association study (GWAS) loci related to different lipid levels in the Indian population and demonstrate the combined effect of variants for lipid traits among Indians by deriving the polygenic risk scores. Similar studies among different populations are required to validate the GWAS loci and effect modification of these loci by lifestyle and environmental factors related to urbanization.</p
Prevalence of risk factors for adverse events, and evidence of their management among participants with CKD.
No full textOf the 558 and 122 participants with CKD in CARRS and MASALA respectively, 430 (77%) and 119 (98%) had complete data on prevalence of risk factors for progression of CKD and/or cardiovascular events. While 43% of participants with CKD in CARRS had diabetes, only 17% were on medications and only 2% (i.e., 4% of those with CKD and diabetes) had A1c < 7.0.</p
Prevalence of chronic kidney disease in the CARRS and MASALA studies.
No full textPrevalence of chronic kidney disease in the CARRS and MASALA studies.</p
