339 research outputs found
The FUP Credibility: a user satisfaction survey
No full textThis research deals with electronic publishing contemporary academic context. In particular the focus of the research is to verify the degree of credibility of Firenze University Press (FUP), academic electronic press project of the University of Florence, investigating its users’ perceptions and opinions. This survey was motivated by the need of FUP to develop a strategy focused to rationalise resources, responding to users requirements. Survey investigated mainly two categories of FUP user: professors as customers, authors (or potential authors) and students, as customers. Mixed method have adopted to draw the survey model, that combining qualitative and quantitative approaches, use both of them. Sample survey methodology have been used to obtain a picture of the general trend of faculty of the University of Florence, by selecting and measuring a sample from the whole population. Research have been directed to a sample of ‘electronically oriented’ users, those who have no prides towards Internet and email as information and communication tools and let personal email to be visible on the University website. On the basis of data and opinions gathered emerged:
1. high percent of user satisfaction;
2. promotional factors:
Primary
- high visibility
- copyright guarantee
Secondary
- speediness
- easiness
- extended access;
3. recommendations:
a) Activity of promotion and growth of visibility
b) Collaboration with commercial publishers, other University Presses
c) Production scheme
d) Editing
e) Access and sellin
Optimales Containment
No full textWe study algorithmic aspects of optimal containment problems in this
thesis. We consider 1- and k-containment problems under homothety, with the
special case of the k-center problem, as well as rotational containment
problems, especially some problems involving cylinders. We discuss the
complexity of such problems alongside algorithms to compute bounds for the
optimal value, relying amongst other on geometric inequalities, mathematical
programming formulations, and the concept of core-sets to achieve these
results. The thesis includes experimental studies of the practical performance
of the described algorithms, too.Thema dieser Arbeit sind optimale Containment-Probleme unter
algorithmischen Gesichtspunkten. Es werden 1- und k-Containment-Probleme
unter Homothetie betrachtet, darunter der Spezialfall des k-Center-Problems,
sowie Containment-Probleme unter Rotation, insbesondere solche mit
Zylindern. Dabei sind sowohl die Komplexität der Probleme als auch
Algorithmen zur Näherung des Optimalwerts von Interesse. Hierzu werden unter
anderem geometrische Ungleichungen, Formulierungen als mathematische
Programme, sowie Core-Sets eingesetzt. Die Arbeit enthält weiterhin Tests
zur praktischen Überprüfung der beschriebenen Algorithmen
Site directed mutagenesis for the identification of sequence differences between chalcone 3-hydroxylase and flavonoid 3'-hydroxylase
No full textChalkon 3-hydroxylase und Flavonoid 3'-hydroxylase sind Cytochrom P450 abhängige Monooxygenasen, die im B-Ring von Chalkonen und/oder verschiedenen Flavonoidklassen eine zusätzliche Hydroxylgruppe an der Position 4 (Chalkone) bzw. 4' (Flavonoide) zu der bereits vorhandenen OH-Gruppe einführen. Während die F3'H keinen Umsatz mit Chalkonen zeigen weist die CH3H eine breitere Substratakzeptanz auf, da sie sowohl Chalkone als auch Flavonoide umsetzt. Im Rahmen dieser Arbeit wurden sieben cDNÄKlone aus Bidens ferulifolia, Cosmos sulphureus, Dahlia variabilis und Coreopsis grandiflora bearbeitet. Bei den drei erstgenannten Pflanzen waren Paare aus CH3H und F3'H vorhanden, während bei Coreopsis grandiflora nur eine Aminosäuresequenz unbekannter Substratspezifität vorlag. Fünf der sieben verfügbaren cDNA Klone lagen bereits in einem Plasmid vor, der einen C-terminalen Strep-Tag aus acht Aminosäuren enthielt. Die cDNA Klone CH3H aus D. variabilis sowie F3'H aus C. sulphureus mussten erst mit dem Strep-Tag versehen werden. Sequenzunterschiede zwischen den Proteinen wurden durch zielgerichtete Mutagenese auf ihren Einfluss auf die Substratakzeptanz bzw. -spezifität abezüglich Isoliquiritigenin, Apigenin Dihydrokämpferol, Kämpferol und Naringenin untersucht. Im Rahmen der Diplomarbeit wur- den bestimmte Positionen in den Aminosäuresequenzen als potenziell relevant für die Funktionalität der Enzyme identifiziert. Insbesondere die Modifikation an der Position 136 verursachte eine erhebliche Änderung der Aktivität bei drei der untersuchten Enzyme (C. sulphureus CH3H und F3'H sowie C. grandiflora). Wie aus der Untersuchung der Sequenzen hervorging, enthielten alle Enzyme mit Ausnahme des aus C. grandiflora isolierten an Stelle 136 Prolin während sich in letzterem ein Serin an der genannten Position befand. Durch diesen gezielten Austausch bei C. grandiflora konnte ein mit nur geringem Naringenin-Umsatz fast inaktives Enzym in ein funktionelles aktives Enzym umgewandelt werden. Der entgegengesetzte Austausch von Serin in Prolin an der Position 136 führte bei C. sulphureus CH3H und F3'H zu einem inaktiven Enzym. Andere untersuchte Positionen, die sich zum größten Teil in oder in der Nähe der verschiedenen Substraterkennungsstellen befinden, erwiesen sich als wenig einflussreich auf die Substratakzeptanz bzw. -spezifität.Chalcone 3-hydroxylase (CH3H) and flavonoid 3'-hydroxylase (F3-H) are cytochrome P450 dependent monooxygenases. Chalcones and/or other flavonoidclasses carry an extra hydroxyl group at position 4 (chalcone) or 4' (flavonoid) to the already existing hydroxyl group in ring B. While F3'H shows no conversion of chalcones, CH3H accepts a broad range of substrates, and hydroxylates both chalcones and flavonoids. In this thesis, seven cDNA clones from Bidens ferulifolia, Cosmos sulphureus, Dahlia variabilis and Coreopsis grandiflora were studied. Pairs of CH3H and F3'H from the first three plant species and a single F3-H cDNA clone from C. grandiflora were available. From the seven cDNA clones, five were already present in the pIBA51 vector conveying the C-terminal strep-tag of eight amino acids. The other two cDNA clones encoding CH3H D. variabilis and F3'H C. sulphureus were subcloned into the the pIBA51 vector. Sequence differences between the translated proteins putatively determining substrate specificity were analyzed by site-directed mutagenesis. The substrate specificity of resulting recombinant mutated proteins were tested with the five (14C)-labelled substrates isoliquiritigenin, apigenin, dihydrokaempferol, kaempferol and naringenin. It could be shown that substrate specificity is not determined by single locations but must be a result of the interaction of several amino acids in the N-terminal regions. Position 136 in the C. grandiflora amino acid sequence was identified as potentially relevant for the functional activity of this enzyme type. A highly conserved serine is present in all CH3Hs and F3-Hs, whereas the C.grandiflora F3-H showed a proline in this position. This results in an inactive enzyme as shown by site-directed mutagenesis. In this work the importance of the serine in position 136 was confirmed by introducing the proline in the CH3H and F3-H of Cosmos sulphureus. This triggered the opposite effect and resulted in functionally inactive enzymes
[en] THE ROLE OF THE AUTHOR OF COURSE BOOKS FOR ENGLISH LANGUAGE TEACHING: A STUDY OF AUTHORIAL IDENTITY
No full textRushlight: Volume IX, June 1947, Graduation Issue
No full textWheaton College (Norton, MA) student literary magazine.Our Team Will Never FailLove Suffers SophisticationThe IntersectionAn Essay On World GovernmentA Group of PoemsThe Honey DripperA Sunday AfternoonDead BirdBoleroDanger...Danger...Fishe
Synthesis and Characterization of MCM-41 spheres inside bioactive glass-ceramic scaffold
No full textComposite systems have been prepared, combining the drug up-take and release properties of MCM-41 silica sub-micron spheres with those of a bioactive glass-ceramic macroporous scaffold belonging to the SiO2-CaO-K2O (SCK) system (potentially able to promote successful osteointegration in tissue engineering). The systems are prepared by dipping the glass-ceramic scaffold into the MCM-41 synthesis solution. To this purpose, the pH of the MCM-41 synthesis solution has been lowered with respect to the usual literature procedure to minimize the detrimental effects of the impregnation treatment on glass-ceramic scaffolds that were observed in a previous work by the author. MCM-41-SCK composite scaffolds have been characterized by means of XRD, N2 adsorption, thermogravimetry and Scanning Electron Microscopy (coupled with EDS analysis). The adsorption capacity toward ibuprofen, tested as model drug, is three times higher than that of the MCM-41-free scaffold, because of the presence of the ordered mesoporous silica. Also the release behaviour in SBF at 37◦C is strongly affected by the presence of MCM-41 inside the scaffold macropore
Recombinant production of Cytochrome P450 dependent enzymes involved in plant pigment formation in microbial expression hosts
No full text
Authority control. Teorie, applicazioni e prospettive di sviluppo
No full textThe authority control, though being a process which integrates the catalogue architecture – and which assures the indexes quality (author, title, descriptor) and supports the relational and syndetic structure of the catalogue, endorsing capabilities of fully retrieving the information it contains – for a long time has been disregarded in the context of the theoretic thought of the catalogue pertaining process. During the last years this trend was reversed: the authority control gained a first level role on the international bibliotheconomic scenario; at the present time in this area there are many operative plans and new applications, often managed in a co-operative and trans-national way, in some cases stimulating the authority control to extend its range of action outside libraries, in order to interoperate with heterogeneous systems like archives, e-commerce systems and museums.
This work wants to define the role and the functions of the nominal and semantic authority control. The first part deals with the authority control under the theoretic point of view: all its elements are examined, while its origin and development in the cataloguing process and in relation with the organisation and relational structure of the catalogue is outlined. The second part treats the semantic indexes control; after a short theoretic-normative report, the situation of the authority control in Italy, which has today, like it never had, a particular evolution, is deeply examined. Referring to the Sistema Biliotecario Nazionale (SBN, National Library Service), the story of the evolution of the semantic authority control in Italy is sketched, along with the new development perspectives favoured by the Nuovo Soggettario project. At the end this work treats what should be considered the most important existing project, on a co-operative international basis, of subject authority control: the Subject Authority Cooperative Program (SACO)
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