1,721,015 research outputs found

    Use of the polymerase chain reaction to clone the potato leafroll virus coat protein gene directly from the total RNA of infected plants

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    The potato leafroll virus (PLRV) coat protein (CP) gene was directly cloned from the total RNA extracted from virus-infected plants. First strand cDNA synthesis was not necessarily specific; it was equally efficient using either random or CP-specific primers. The viral sequence encoding the coat protein was specifically amplified from the total population of cDNA molecules by polymerase chain reaction (PCR), using specific primers bordering the CP gene. The unique amplified product thus obtained was cloned blunt-end into the pT7T318U plasmid vector, and the authenticity of the cloned gene verified by sequence analysis. This cloning strategy obviates the need for virus purification. Sequence comparison of the CP gene of the Italian isolate and those of five other PLRV isolates revealed a close similarity to the three European and the Canadian isolates, and a more distant relationship with the Australian one. © 1995 Kluwer Academic Publishers

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Regional aspects of decision-making support for rural development in Poland

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    Measures for rural development should be adapted to the specific regional conditions and national programs should allow for different regional priorities. However, decision-making for policy measures often takes place under special conditions with many concerned actors, unstructured decision problems and time pressure. These conditions, decision-makers in administrations and institutions are faced with, make the formation of policy-measures for rural development a complex matter. Thus, there is the question arising how decision-makers can be supported in setting priorities for allocating budgets for policy measures among regions. Recently, multi criteria decision-making approaches are discussed to tackle these kinds of decision problems. We show exemplarily for the Polish program of rural development, how decision-making could be supported using a multi-objective programming approach. Different preferences of actors can be considered explicitly by visualizing “trade-offs” and an interactive use of the approach. For example, a political "equity" objective is implemented as a constraint in the programming approach, restricting the budget differences between regions to a defined level. By a parameterization of the bound for budget differences, the "trade-off" between three objectives is displayed and evaluated. Using the exemplary programming approach, it is shown that the objective values of the two main objectives of the PROW decline, when the budget differences between regions are restricted for pursuing a political "equity" objective.Regional Budgeting, Interactive Decision-making support, Multi-objective Programming (MOP), Community/Rural/Urban Development,

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    David ROSNER, A Once Charitable Enterprise. Hospitals and Health Care in Brooklyn and New York, 1885-1915

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    Zunz Olivier. David ROSNER, A Once Charitable Enterprise. Hospitals and Health Care in Brooklyn and New York, 1885-1915. In: Annales. Economies, sociétés, civilisations. 40ᵉ année, N. 1, 1985. pp. 164-165

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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