323,245 research outputs found
Clinical practice evaluation of combination of atosiban, ritodrine and ketoprofen for inhibiting preterm labor.
No full textMinerva Ginecol. 2007 Oct;59(5):481-9.
Clinical practice evaluation of combination of atosiban, ritodrine and ketoprofen for inhibiting preterm labor.
Grignaffini A, Soncini E, Ronzoni E, Lo Cane F, Anfuso S, Nardelli GB.
SourceDepartment of Gynaecology, Obstetrics and Neonatology, University of Parma, Parma, Italy.
Abstract
AIM: The aim of this study was to compare the efficacy and tolerability of atosiban vs ritodrine administered as single-drug or as combination therapy with the COX inhibitor ketoprofen in the treatment of preterm labor and to investigate how frequent is the need for combination therapy with ketoprofen.
METHODS: Ninety-one women with diagnosis of threatened preterm delivery at 24-33 weeks' gestation were enrolled in an observational case-control study. Forty-seven received IV atosiban (6.75 mg initial dose, 300 microg/min loading dose for 3 hours, 100 microg/min maintenance dose for 48-96 hours) and 44 IV ritodrine (0.05-0.3 mg/min). When response to the first drug in the first 2-4 hours was unsatisfactory, ketoprofen was added (100 mg loading dose IV and 100-150 mg maintenance dose every 12 hours) for a maximum of 48 hours.
RESULTS: Ketoprofen was added in 51.1% of the atosiban group and 47.7% of the ritodrine group (P 0.75, not statistically significant). The percentages of women non delivered in the two groups were 85.1% vs 81.8% at 48 hours (P=0.44) and 59.6% vs 54.5% at 7 days (P=0.39). One woman treated with atosiban reported transient dyspnea at the administration of the bolus dose; 20.5% of women who received ritodrine developed tachycardia and 4.5% dyspnea (P=0.001). Neonatal mortality and morbidity were comparable in both groups and unrelated to ketoprofen exposure.
CONCLUSION: Atosiban efficacy was comparable to ritodrine, but with a superior safety profile. A large proportion of women in both groups required second-line ketoprofen therapy, with comparable neonatal outcome
Synthesis of Pyrazoles by 1,3-Dipolar Cycloaddition under Aqueous Micellar Catalysis
No full textEthyl diazoacetate (EDA), which is easily prepared from ethyl glycinate and NaNO2, reacts in situ with alkynes in a water micelle environment without organic solvent to form pyrazoles. The reaction is pH dependent, as in the presence of protic catalysis (H2SO4 4%, pH 3.5) a mixture of 3,5- and 4,5-disubstituted pyrazoles was obtained, while, at pH 5.5, only the 3,5-disubstituted isomer was obtained. The presence of the surfactant TPGS-750-M was crucial to secure clean crude reaction mixtures and high yields of the products. The same protocol was successfully applied to the synthesis of substituted pyrazolines. © 2022 The Author
STRUCTURAL STUDIES OF MOLLUSK AND MAMMALIAN HEPARINS - HEPARAN SULFATES AND THEIR DISACCHARIDE UNITS BY NMR-SPECTROSCOPY
No full textESCOLA PAULISTA MED SCH,DEPT BIOQUIM,BR-04023 SAO PAULO,SP,BRAZILMCGILL UNIV,DEPT CHEM,MONTREAL H3A 2T5,QUEBEC,CANADAIST QUIM & BIOQUIM G RONZONI,MILAN,ITALYESCOLA PAULISTA MED SCH,DEPT BIOQUIM,BR-04023 SAO PAULO,SP,BRAZILWeb of Scienc
MAINTENANCE of HEPARAN-SULFATE STRUCTURE THROUGHOUT EVOLUTION - CHEMICAL and ENZYMIC DEGRADATION, and C-13 NMR-SPECTRAL EVIDENCE
No full textIST CHIM & BIOCHIM G RONZONI,MILANO,ITALYESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04034 São Paulo,SP,BRAZILESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04034 São Paulo,SP,BRAZILESCOLA PAULISTA MED,DEPT BIOQUIM,BR-04034 São Paulo,SP,BRAZILWeb of Scienc
New method to detect histone acetylation levels by flow cytometry
No full textBackground: Reversible histone acetylation affects chromatin structural organization, thus regulating gene expression and other nuclear events. Levels of histone acetylation are tightly modulated in normal cells, and alterations of their regulating mechanisms have been shown to be involved in tumorigenesis. Methods: We developed a new flow cytometric technique for detection of histone acetylation, based on a specific monoclonal antibody that recognizes acetylated histone tails. Bivariate analysis for histone acetylation levels and DNA were performed to study modulation of chromatin organization during the cell cycle and after induction of histone hyperacetylation by the histone deacetylase (HDAC) inhibitor trichostatin A (TSA). Histone acetylation and transcription levels were monitored during differentiation induced by retinoic acid alone or in combination with TSA. Blood samples from patients were analyzed with the described protocol to monitor the effects of HDAC inhibitors in vivo and validate the developed protocol for clinical usage. Results: Flow cytometric detection of acetylation status can successfully detect modifications induced by HDAC inhibitor treatment in vivo as demonstrated by analysis of various blood samples from patients treated with valproic acid. Changes in acetylation levels during the cell cycle demonstrated a reproducible increase in histone acetylation during the replication phase that was subsequently decreased at the G2M entrance, thus paralleling the behavior of DNA replication and transcriptional activity. Conclusions: Multiparameter analysis of histone acetylation and expression of molecular markers, DNA ploidy, and/or cell cycle kinetics can provide a quick and statistically reliable tool for the diagnosis and evaluation of treatment efficacy in clinical trials using HDAC inhibitors
Assessment of histone acetylation levels in relation to cell cycle phase
No full textHistone acetylation affects chromatin structural organization, thus regulating gene expression and DNA-related cellular events. Levels of histone acetylation are tightly modulated in normal cells and frequently altered in tumors. Consequently, histone deacetylase inhibitors are currently being tested in clinical trials as anticancer drugs. Presented here is a protocol for measuring the degree of cellular histone tail acetylation, alone or in combination with DNA content to simultaneously evaluate cell ploidy and/or cell cycle progression. The procedure can also be employed to stain peripheral blood samples in order to assess mean histone acetylation levels in patients treated with histone deacetylase inhibitor
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