2,166 research outputs found
Overactivity of exercise-sensitive cation channels and their impaired modulation by IGF-1 in mdx native muscle fibers: beneficial effect of pentoxifylline
No full textAbstract
Cell-attached patch-clamp recordings on native striated myofibers from adult dystrophic mdx mice revealed a higher occurrence and open probability compared to non-dystrophic wild-type myofibers of a 30 pS voltage-insensitive Ca2+-permeable channel, inhibited by Gd3+, streptomycin and ruthenium red. Myofibers from in vivo exercised animals had higher channel occurrence and/or open probability. Insulin-like growth factor 1 (3.3 nM) induced and/or enhanced channel activity, via PI3 kinase, in wild-type but not in mdx myofibers. Interestingly, in both genotypes the current was silenced by db-cAMP or pentoxifylline, a phosphodiesterase inhibitor. The channel activity/occurrence in pentoxifylline-treated exercised mdx (50 mg/kg/day i.p. for 4-8 weeks) overlapped that of exercised wild-type mice. Thus, a growth factor-sensitive current, likely due to a TRP channel, is activated in vivo by exercise in native striated fibers; its deregulation in the absence of dystrophin may contribute to Ca2+ homeostasis alteration. The possibility to pharmacologically counteract abnormal channel activity discloses important therapeutic application
TRP-like channels in native wt and mdx muscle fibers: differences in activity and IGF-1 responsiveness
No full textFiber type-related changes in rat skeletal muscle calcium homeostasis during aging and restoration by growth hormone
No full textData on protein abundance alteration induced by chronic exercise in mdx mice model of Duchenne muscular dystrophy and potential modulation by apocynin and taurine
No full textHere we present original data related to the research paper entitled "Proteome analysis in dystrophic mdx mouse muscle reveals a drastic alteration of Key Metabolic and Contractile Proteins after chronic exercise and the potential modulation by anti-oxidant compounds" (Gamberi et al., 2018) [1]. The dystrophin-deficient mdx mouse is the most common animal model for Duchenne muscular dystrophy. The mdx mice phenotype of the disorder is milder than in human sufferers and it can be worsened by chronic treadmill exercise. Apocynin and taurine are two antioxidant compounds proved to be beneficial on some pathology related parameters (Schroder and Schoser, 2009) [2]. This article reports the detailed proteomic data on protein abundance alterations, in tibialis anterior muscle of mdx mice, induced by chronic exercise protocol. A selected group of mdx mice was also treated with apocynin and taurine during this protocol. Detailed MS data, comparison between mdx vs wild type, exercised mdx vs wild type, and complete analysis of spot variation are provided. Furthermore, in wild type mice subjected to the same exercise protocol, the abundance of key proteins, resulted modified in exercised mdx, were analyzed by western blot. (C) 2018 The Authors. Published by Elsevier Inc
Evaluation of potential synergistic action of a combined treatment with alpha-methyl-prednisolone and taurine on the mdx mouse model of Duchenne muscular dystrophy.
No full textAims: Glucocorticoids are the sole drugs clinically used
in Duchenne muscular dystrophy, in spite of the relevant
side effects. Combination of glucocorticoids with synergistic
drugs may be one strategy to lower doses and
control side effects, meanwhile providing wider control
of the complex pathology. This study is a preclinical
evaluation of the effect of a combined treatment of amethyl-
prednisolone (PDN) with taurine, a safe aminoacid
with positive effects on some pathology-related
events. Methods: PDN (1 mg/kg/day i.p.) and taurine
(1 g/kg/day orally) were administered either alone or in
combination, for 4–8 weeks to male dystrophic mdx mice
chronically exercised on a treadmill. Effects were assessed
in vivo and ex vivo with a variety of methodological
approaches. Results: In vivo, each treatment significantly
increased fore limb strength, a marked synergistic effect
being observed with the combination PDN + taurine. Exvivo, PDN + taurine completely restored the mechanical
threshold, an electrophysiological index of calcium
homeostasis, of extensor digitorum longus myofibres and
the benefit was greater than for PDN alone. In parallel,
the overactivity of voltage-independent cation channels
in dystrophic myofibres was reduced. No effects were
observed on plasma levels of creatine kinase, while
lactate dehydrogenase was decreased by taurine and, to a
minor extent, by PDN + taurine. A similar histology
profile was observed in PDN and PDN + taurine-treated
muscles. PDN + taurine significantly increased taurine
level in fast-twitch muscle and brain, by high-pressure
liquid chromatography analysis. Conclusions: The combination
PDN + taurine has additive actions on in vivo
and ex vivo functional end points, with less evident
advantages on histopathology and biochemical markers
of the disease
Growth hormone secretagogues induce intracellular calcium mobilization in native rat skeletal muscle fibers through ghrelin-specille receptor activation
No full textA new original compound, which inhibits calpain 1-2 and reactive oxygen species, reduces the dystrophic progression of mdx mice
No full textBusiness Model Innovation of JF Logistics Company
No full text摘要 随着全球化经济的发展，市场竞争变得越来越复杂。信息时代使得物流供应链管理已上升到企业的战略管理高度。在这样的背景下，本文应用翁君奕老师的介观商务模式创新观点，对JF物流公司所处行业现状进行剖析，重新审视了外部客户市场以及内部自身情况，找出了JF物流公司自身的优势，并结合外部市场客户的需求，提出了“为客户提供个性化的集物流、资金流、信息流于一体的供应链物流服务”这一价值主张，并在此基础上，重新定位客户市场，创新服务产品，理顺内部管理架构和业务流程以支撑和保持这一价值主张。文中同时以例证来说明依据新价值主张所创新的服务产品给JF物流公司所带来的变化，以此说明通过商务模式创新来实行自身的战略...Abstract With the development of the global economy, the competition in market becomes more complicated. In the era of information, logistics and supply chain management is regarded as important as part of the company strategy. Under such background , the author of this essay uses the concept of “JieGuan Business Model Innovation” proposed by Professor Weng Junyi of Xiamen University, and analy...学位：管理学硕士院系专业：管理学院高级经理教育中心（EMBA项目）_管理经济学学号：X200615614
Temporal and spatial variability in speakers with Parkinson's Disease and Friedreich's Ataxia
No full textSpeech variability in groups of speakers with Parkinson's disease (PD) and with Friedreich's ataxia was compared with healthy controls. Speakers repeated the same phrase 20 times at one of two rates (fast or habitual). A non-linear analysis of variability was performed which used some of the principles behind the spatio-temporal index (STI). The STI usually employs variation in lip displacement over repetitions of the same utterance and a linear analysis of such signals is conducted to represent the combined variation in spatial and temporal control. When working with patients, audio measures (here we used speech energy) are preferred over kinematics ones as they are minimally disruptive to speech. Non-linear methods allow spatial variability to be estimated separately from temporal variability. The results are tentatively interpreted as showing that PD speakers were distinguished from healthy control speakers in spatial variability and ataxic speakers were distinguished from controls in temporal variability. These findings are consistent with the speech symptoms reported for these disorders. We conclude that the non-linear analysis using the speech energy measure is worth investigating further as it is potentially revealing of the differences underlying these two pathologies
Additional file 6 of Prevalence of chronic cough in China: a systematic review and meta-analysis
No full textAdditional file 6. Fig. S1. Distribution of children with chronic cough across Mainland China. NOTE: Red star in the map represents Beijing City. The map was developed in XL Toolbox NG by ourselves, without the conflict of copyright. Fig. S2. Pooled chronic cough prevalence of adults stratified by region. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S3. Pooled chronic cough prevalence of adults stratified by diagnostic criteria. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S4. Pooled chronic cough prevalence of adults stratified by year of publication. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S5. Pooled chronic cough prevalence of adults stratified by age. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S6. Pooled chronic cough prevalence of adults stratified by sampling methods. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S7. Pooled chronic cough prevalence of adults stratified by sample size. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S8. Pooled chronic cough prevalence of adults stratified by prevalence definitions. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S9. Pooled chronic cough prevalence of adults stratified by chronic cough definitions. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S10. Pooled chronic cough prevalence of adults stratified by quality of articles assessed by AHRQ. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S11. Pooled chronic cough prevalence of children stratified by region. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S12. Pooled chronic cough prevalence of children stratified by diagnostic criteria. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S13. Pooled chronic cough prevalence of children stratified by year of publication. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S14. Pooled chronic cough prevalence of children stratified by sample size. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S15. Pooled chronic cough prevalence of children stratified by chronic cough definitions. Abbreviations: CI, confidence intervals; ES, Effect Size. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S16. Pooled chronic cough prevalence of children stratified by quality of articles assessed by AHRQ. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S17. Pooled chronic cough prevalence of children stratified by prevalence definitions. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S18. Funnel plot for prevalence in studies of adults for chronic cough. Fig. S19. Sensitivity analysis for prevalence in studies of adults for chronic cough. Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S20. The prevalence of chronic cough in adults after exclusion of the nationwide study (Li JC 2018). Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S21. The prevalence of chronic cough in adults after exclusion of the low prevalence study (ZHANG JF 1999). Abbreviations: CI, confidence intervals. NOTE: The two author labels of ZHANG JF 1999 are from the same literature, and the two author labels of Venners 2001 are from the same literature. Fig. S22. Funnel plot for prevalence in studies of children for chronic cough. Fig. S23. Sensitivity analysis for prevalence in studies of children for chronic cough. Abbreviations: CI, confidence intervals. NOTE: The four author labels of ZHANG JF 2002 are from the same literature. Fig. S24. Pooled prevalence of chronic cough in China (including adults and children). Abbreviations: CI, confidence intervals. NOTE: The three author labels of ZHANG JF 1999 are from the same literature, the two author labels of Venners 2001 are from the same literature, and the four author labels of ZHANG JF 2002 are from the same literature
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