1,720,993 research outputs found
Effect of bioactive glasses on Angiogenesis: a review of in vitro and in vivo evidences
Full text linkThe incorporation of bioactive glass into bone tissue-engineered scaffolds can be widely beneficial based on emerging evidence in the literature about the angiogenic potential of this material, particularly 45S5 Bioglass . This article reviews the literature discussing in vitro studies which have demonstrated that increases in angiogenic indicators have been achieved through both direct and indirect contact of relevant cells with 45S5 Bioglass particles or with their dissolution products. A few available in vivo studies confirming the ability of bioactive glass, incorporated into scaffolds, to stimulate neovascularization are also discussed. Suggestions for further research are given, highlighting the need for specific investigations designed to assess the effect of particular ion dissolution products from bioactive glasses and their relative concentration on angiogenesis both in vitro and in vivo.Fil: Gorustovich Alonso, Alejandro Adrian. Comision Nacional de Energia Atomica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roether, Judith A.. Imperial College London; Reino UnidoFil: Boccaccini, Aldo R.. Imperial College London; Reino Unid
Mending a broken heart by biomimetic 3D printed natural biomaterial-based cardiac patches: a review
Full text link: Myocardial infarction is one of the major causes of mortality as well as morbidity around the world. Currently available treatment options face a number of drawbacks, hence cardiac tissue engineering, which aims to bioengineer functional cardiac tissue, for application in tissue repair, patient specific drug screening and disease modeling, is being explored as a viable alternative. To achieve this, an appropriate combination of cells, biomimetic scaffolds mimicking the structure and function of the native tissue, and signals, is necessary. Among scaffold fabrication techniques, three-dimensional printing, which is an additive manufacturing technique that enables to translate computer-aided designs into 3D objects, has emerged as a promising technique to develop cardiac patches with a highly defined architecture. As a further step toward the replication of complex tissues, such as cardiac tissue, more recently 3D bioprinting has emerged as a cutting-edge technology to print not only biomaterials, but also multiple cell types simultaneously. In terms of bioinks, biomaterials isolated from natural sources are advantageous, as they can provide exceptional biocompatibility and bioactivity, thus promoting desired cell responses. An ideal biomimetic cardiac patch should incorporate additional functional properties, which can be achieved by means of appropriate functionalization strategies. These are essential to replicate the native tissue, such as the release of biochemical signals, immunomodulatory properties, conductivity, enhanced vascularization and shape memory effects. The aim of the review is to present an overview of the current state of the art regarding the development of biomimetic 3D printed natural biomaterial-based cardiac patches, describing the 3D printing fabrication methods, the natural-biomaterial based bioinks, the functionalization strategies, as well as the in vitro and in vivo applications
Bioactive glasses for soft tissue engineering applications
No full textIn the last few years the usage of bioactive glasses as scaffolds for soft tissue engineering has been investigated more thoroughly. The reason for the boost in interest are the attractive properties bioactive glasses offer including bioactivity as well as antibacterial, angiogenic and hemostatic properties. So far, most research efforts have focussed on applications for repairing skin and nerve tissue although there have been interesting developments in other fields including lung and intestines, which could potentially benefit a large group of patients but more studies are required. Three comprehensive reviews on this topic have been published recently, so this chapter will mainly focus on the latest relevant published research. There are a great number of patents registered for the use of bioactive glass for hard tissue engineering, however, recently patents detailing the use of bioactive glass for soft tissue engineering applications have been filed, which open the way to market bioactive glasses for the restoration of soft tissues. The angiogenic effect of bioactive glasses is of great interest for tissue engineering applications in general and in particular for soft tissue engineering, hence the third part of this chapter will detail the latest research on the angiogenic properties of bioactive glasses.Fil: Miguez Pacheco, Valentina. Universitat Erlangen Nuremberg; AlemaniaFil: Gorustovich Alonso, Alejandro Adrian. Universidad Católica de Salta; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingenieria "Hilario Fernandez Long". Grupo Vinculado al Intecin - Grupo Interdisciplinario en Materiales; ArgentinaFil: Boccaccini, Aldo Roberto. Universitat Erlangen Nuremberg; AlemaniaFil: Roether, Judith A.. Universitat Erlangen Nuremberg; Alemani
Metal and Metal Oxide Nanoparticle-incorporated Polymer Nanofibers for Wound Healing
No full textThis chapter presents an in-depth overview of polymer nanofibers incorporated with metal and metal oxide nanoparticles for wound healing applications. It begins by outlining the fundamentals of wound healing and the limitations of conventional wound dressings. The electrospinning technique is then introduced as a versatile method for fabricating nanofibrous scaffolds that closely mimic the extracellular matrix, offering enhanced porosity, surface area, and mechanical properties ideal for wound care. This chapter extensively examines the role of metal nanoparticles (AgNPs, CuNPs, AuNPs) and metal oxide nanoparticles (ZnO NPs, TiO2 NPs, Fe3O4 NPs) in enhancing the antibacterial, antioxidant, pro-angiogenic, and anti-inflammatory properties of nanofibrous wound dressings. The mechanisms by which these nanoparticles exert antimicrobial activity – such as reactive oxygen species (ROS) generation, membrane disruption, and metal ion release – are detailed. Furthermore, the chapter analyzes how the incorporation of nanoparticles influences the physicochemical properties of nanofibers, as well as the associated challenges, including cytotoxicity, aggregation, stability, scalability, and regulatory barriers. Future perspectives highlight the potential of greener synthesis methods, the use of biocompatible reducing and stabilizing agents, and the development of synergistic nanoparticle combinations to enhance therapeutic outcomes while mitigating toxicity. This chapter serves as a comprehensive guide for advancing the design of multifunctional wound dressings that address current clinical challenges
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds
Full text linkThis work aimed to develop novel composite biomaterials for bone tissue engineering (BTE) made of bioactive glass nanoparticles (Nbg) and alginate cross-linked with Cu2+ or Ca2+ (AlgNbgCu, AlgNbgCa, respectively). Twodimensional scaffolds were prepared and the nanocomposite biomaterials were characterized in terms of morphology, mechanical strength, bioactivity, biodegradability, swelling capacity, release profile of the cross-linking cations and angiogenic properties. It was found that both Cu2+ and Ca2+ are released in a controlled and sustained manner with no burst release observed. Finally, in vitro results indicated that the bioactive ions released from both nanocomposite biomaterials were able to stimulate the differentiation of rat bone marrow-derived mesenchymal stem cells towards the osteogenic lineage. In addition, the typical endothelial cell property of forming tubes in Matrigel was observed for human umbilical vein endothelial cells when in contact with the novel biomaterials, particularly AlgNbgCu, which indicates their angiogenic properties. Hence, novel nanocomposite biomaterials made of Nbg and alginate cross-linked with Cu2+ or Ca2+ were developed with potential applications for preparation of multifunctional scaffolds for BTE.Fil: Cattalini, Juan Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Hoppe, A.. Universitat Erlangen-Nuremberg; AlemaniaFil: Pishbin, F.. Imperial College London; Reino UnidoFil: Roether, Judith A.. Universitat Erlangen-Nuremberg; AlemaniaFil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
Extracellular Matrix (ECM) Mimicking Hybrid and Composite Nanofiber Materials: A General Perspective on Structural and Chemical Biomimicry and Their Role in Wound Healing and Skin Modelling
No full textThe main purpose of implementing biomaterials for regenerative medicine, e.g., in wound healing, is provision of topographical, biochemical, and biomechanical cues to regulate cell activities and to support cell attachment. In this regard, nature has already established a template, i.e., the extracellular matrix (ECM), which has been inspiring in the creation of different classes of biomaterials. Biomaterials that recapitulate the structure, composition, and dynamics of the ECM have been shown to be effective in tissue regeneration and wound healing applications. In particular, nanofiber materials that can simulate the ECM’s collagen nanofilamentous structure, in terms of topography and biochemistry, are highly attractive not only for regeneration of wounded tissue but also for modelling of human native skin to study disease, aging, and therapeutic conditions. Hybrid or composite nanofibers made of synthetic materials, natural materials or a blend of both can not only be employed as wound healing materials, but also as the basis of the epidermal and dermal layer in three-dimensional (3D) organotypic skin models, a new paradigm for modelling of human skin to identify the efficacy of therapeutics and wound healing processes. In this chapter, hybrid and composite nanofibrous materials are discussed from an ECM biomimicry standpoint (structural and biochemical biomimicry) that is crucial for their wound healing and skin modelling application. In collecting the presented information, we mainly considered the innovations carried out in the past five years to provide an updated overview on the ECM mimicking nanofibrous materials that have been applied as wound dressings, skin substitutes, and 3D skin models
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Development of bioactive glass based scaffolds for controlled antibiotic release in bone tissue engineering via biodegradable polymer layered coating
Full text linkHighly porous 45S5 Bioglass®-based scaffolds coated with two polymer layers were fabricated to serve as a multifunctional device with controlled drug release capability for bone regeneration applications. An interior poly(d,l-lactide)/poly(ethylene glycol)-(polypropylene glycol)-poly(ethylene glycol) triblock copolymer (Pluronic P123) coating improved the mechanical stability of Bioglass-based scaffolds, while an exterior natural polymer (alginate or gelatin) coating served as an antibiotic drug carrier. The results showed improved mechanical properties of Bioglass-based scaffolds by the bilayer polymer coating. In addition, hydrochloride tetracycline loaded in either alginate or gelatin coatings was released rapidly at the initial stage (∼1 h), while the released rate subsequently decreased and was sustained for 14 days in phosphate buffered saline. Therefore, these layered polymer coated scaffolds exhibit attractive characteristics in terms of improved mechanical properties and controlled drug release, simultaneously with the added advantage that the drug release rate is decoupled from the intrinsic scaffold Bioglass degradation mechanism. The layered polymer coated scaffolds are of interest for drug-delivery enhanced bone regeneration applications.Fil: Nooeaid, Patcharakamon. Universitat Erlangen-Nuremberg; AlemaniaFil: Li, Wei. Universitat Erlangen-Nuremberg; AlemaniaFil: Roether, Judith A.. Universitat Erlangen-Nuremberg; AlemaniaFil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Goudouri, Ourania Menti. Universitat Erlangen-Nuremberg; AlemaniaFil: Schubert, Dirk W.. Universitat Erlangen-Nuremberg; AlemaniaFil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemani
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