163,196 research outputs found
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Pseudomonas mevalonii HMG-CoA reductase: Transcriptional regulation and identification of a glutamate essential for catalysis
No full textPseudomonas mevalonii HMG-CoA reductase (E.C. 1.1.1.38) is induced in cells grown on mevalonate (J. F. Gill, Jr., M. J. Beach, and V. W. Rodwell, J. Bacteriol. 160, 294-298, 1984), and the mvaA gene, which encodes P. mevalonii HMG-CoA reductase, has been cloned and sequenced (M. J. Beach and V. W. Rodwell, J. Bacteriol. 171, 2994-3001, 1989). Measurements of HMG-CoA reductase activity, protein, and mRNA revealed that induction is controlled at the transcriptional level. The transcription initiation site is 56 bp upstream of the adenosine of the ATG translation start codon of mvaA. The mvaA promoter has 12 and 24 consensus sequences typical of prokaryotic promoters which utilize sigma\sp{54} RNA polymerase holoenzyme and activator proteins for transcription. The cis-acting element which responds to mevalonate was shown, by assays of the expression of mva-lacZ translation fusions and by DNA gel retardation assays, to be on a 36 pb DNA segment 48 pb upstream of the transcription initiation site. From kinetic data, D. Veloso, W. W. Cleland, and J. W. Porter (Biochemistry 20, 887-894, 1981) inferred that an acidic residue functions in catalysis by yeast HMG-CoA reductase. The catalytic domains of 11 HMG-CoA reductases contain 3 conserved acidic residues. These 3 residues of P. mevalonii HMB-CoA reductase were changed to glutamine (E52 and E83), asparagine (D183), or alanine (D183) by site-directed mutagenesis. All four mutant enzymes were then overexpressed, purified, and characterized. V\sb{\rm max} for mutant enzymes E52Q, D183A, and D183N was 15, 69, and 109% that of wild-type HMG-CoA reductase, respectively. Thus, neither Glu\sp{52} nor Asp\sp{183} is a catalytic residue. Mutant enzymes E52Q and D183A had elevated K\sb{\rm m} values for all substrates and impaired ability to bind to coenzyme A or HMG-CoA affinity supports. By contrast, mutant enzyme E83Q had a V\sb{\rm max} value only 0.4% that of wild-type HMG-CoA reductase. This low value appeared to result neither from an altered conformation nor from an impaired inability to bind substrates. For mutant enzyme E83Q, K\sb{\rm m} values for NAD\sp{+}, R,S-mevalonate, and coenzyme A, and chromatography on affinity supports was typical of wild-type enzyme. The data are consistent with residue E83 of P. mevalonii HMG-CoA reductase being the acidic residue functional in catalysis
Dibdin down the coal hole : G. Herbert Rodwell (1800-1852) and the place of popular song
No full textG. Herbert Rodwell (1800–52), who pursued a double career as a composer and man of letters, turned primarily to novel writing in the 1840s. His three novels are concerned with musical issues linked to Rodwell's general discontent with the status of British composers, as set out in his Letter to the Musicians of Great Britain of 1833. The novels' attitude to popular song is investigated here. Ultimately, Rodwell was advocating both that popular song should be primarily a medium for amateur performers to express themselves, and that composers like himself should be introducing more refined songs into the popular song repertoire.林以知郎先生 Leo J. Loveday先生 御退職記念号Specially dedicated to Professor Ichiro Hayashi and Professor Leo J. Loveday on their retirement論文(Article)departmental bulletin pape
Dibdin down the coal hole : G. Herbert Rodwell (1800-1852) and the place of popular song
No full textG. Herbert Rodwell (1800–52), who pursued a double career as a composer and man of letters, turned primarily to novel writing in the 1840s. His three novels are concerned with musical issues linked to Rodwell's general discontent with the status of British composers, as set out in his Letter to the Musicians of Great Britain of 1833. The novels' attitude to popular song is investigated here. Ultimately, Rodwell was advocating both that popular song should be primarily a medium for amateur performers to express themselves, and that composers like himself should be introducing more refined songs into the popular song repertoire.林以知郎先生 Leo J. Loveday先生 御退職記念号Specially dedicated to Professor Ichiro Hayashi and Professor Leo J. Loveday on their retirement論文(Article)application/pd
A Map Ancient and modern of the Peloponnesus, and of a part of Northern Greece. J & C. Walker / sculp
No full textÉchelle(s) : [1:377 920 environ
Measure for Measure
No full textThe TIFF was created at 1200 dpi and 8-bit grayscale.Characters from Measure for Measure Act 2 Scene 1. Published by Rodwell and Martin, London 1822
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
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