3,977 research outputs found

    Suppression of the Pth(Ts) phenotype mediated by the overproduction of tRNA maintains moderate levels of the Pth(Ts) protein

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    <p><b>Copyright information:</b></p><p>Taken from "Excess of charged tRNA maintains low levels of peptidyl-tRNA hydrolase in (Ts) mutants at a non-permissive temperature"</p><p>Nucleic Acids Research 2006;34(5):1564-1570.</p><p>Published online 15 Mar 2006</p><p>PMCID:PMC1408313.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Depicts the cellular growth of the (Ts) mutant strain AA7852 separately transformed with pVH124 (ΔU, ΔV), pVH125 (U, ΔV) or pVH119 (U, V) incubated at different temperatures. Isolated colonies of the independent transformants were streaked onto LB-Ap plates and incubated overnight at the indicated temperatures. () Presents the immunodetection of Pth(Ts) in the (Ts) mutant strain AA7852 separately transformed with pVH124, pVH125, pVH119, ptRNACCA (X, R, T, M) or pTH2 (W) and grown at 32°C prior to transfer at time = 0 min at 41 or 43°C. The concentration of Pth(Ts) protein was estimated by immunoblot analysis. The left lane shows purified wild-type Pth protein, which migrates slightly faster in SDS–PAGE than the Pth(Ts) variant (arrowed) ()

    Emergence of oblique TS mode due to longitudinal wall oscillation in 2D channel flow

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    Stabilizing effects of wall oscillation on two dimensional (2D) Tollmein-Schlichting (TS) waves and oblique TS waves developing in 2D channel flow are numerically investigated using the Floquet method. It was shown from the previous study[1] that the wall oscillation mostly has stabilizing effect and then TS mode is occasionally more stable than the oblique mode. In the present study the characteristics of the stability are investigated in detail. Then it is cleared that the exchange of the most unstable mode form the 2D TS to the oblique TS can occur at a certain value of Reynolds number

    ISO/TS 16949: analysis of the diffusion and current trends

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    The automotive industry has always shown a particular interest toward quality management systems, which resulted in the development of several different specific standards. As a result of this, by the mid-1980s, automotive suppliers were subject to numerous national and customer specific regulations. The proliferation of these standards and the need to create a single reference model led to Technical Specification (TS) 16949, an ISO technical specification aimed at representing a comprehensive quality management system for the global automotive industry. Since its early introduction, TS 16949 has encountered a certain success thanks to its feature of unifying and harmonizing the already existing standards. This paper studies the global evolution and diffusion of this technical specification, observing its impact on the local economies. The argument is supported by many empirical data

    A TS uszályhíd kifejlesztése és alkalmazása

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    The essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers.The essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers

    The new CEN/TS 19100: Design of glass structures

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    Since the beginning of 2021, CEN/TS 19100 Design of Glass Structures has been available in its first three parts. The fourth part is expected soon. This Technical Specification of the European standards organisation CEN is as a pre-standard of a corresponding future Eurocode. These documents constitute the first ever comprehensive design code for the entire structural glass engineering field on the European market for the first time. In addition to a clear outline, the Technical Specification has been drafted to be compatible with EN 1990 “Basis of Design” and to address glass-specific design matters, particularly related to robustness and redundancy. Although the standard still has the status of a CEN/TS, thereby allowing the European nations the option of whether to introduce it, either in full or in parts, it already contains national openings through which the European countries can adapt the design results to their own safety level by National Determined Parameters (NDPs). Such an approach already anticipates the future Eurocode, which is expected to be published as EN 19100—Design of Glass Structures. This article provides some context on the history and concept behind the new documents and gives an overview of the design rules and the corresponding technical background of the different parts of CEN/TS 19100.Applied MechanicsArchitectural Technolog

    A &quot;tradução&quot; do paradigma etiológico de criminologia no Brasil: um diálogo entre Cesare Lombroso e Nina Rodrigues da perspectiva centro-margem

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    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Jurídicas, Programa de Pós-Graduação em Direito, Florianópolis, 2015.A presente dissertação tem por objetivo principal compreender qual foi e como se operalizou a ?tradução? (processo complexo que inclui várias técnicas, se afastando, assim, da mera recepção) da teoria lombrosiana e principalmente do paradigma fundado pela Criminologia Positivista, funcional ao controle social no Centro (Europa), realizada por Raimundo Nina Rodrigues para o controle racial na Margem (Brasil) no pós-abolição. Em seu contexto original, aquele paradigma, consolidado na obra L?Uomo Delinquente, possibilitou a seleção e segregação de uma minoria ?anormal? visando seu disciplinamento através de estabelecimentos correcionais, mas sua concepção se encontra na primeira obra de Lombroso, L?uomo bianco e l?uomo do colore: letture sull?origine e la varietà delle razze umane, cuja tradução literal inédita se faz necessária para a compreensão pretendida, procurando, além do resgate de seu racismo negado, marcar as permanências e rupturas (se houver) durante a travessia atlântica. Nesse trajeto, desvelamos a construção de seus ?Outros? que nos leva à construção, muito além do centro, de sua espécie mais primitiva: o negro. Para ter sua funcionalidade assegurada em um contexto periférico singular, marcado pelo recente fim do maior sistema escravagista do mundo, a teoria do criminoso nato possibilitou a manutenção da ordem racial atravessando o quadro teórico liberal da jovem República ao ter retomada sua matriz racista, reforçada e potencializada pelo médico brasileiro, legítimo representante da classe escravagista, pelo ecletismo teórico-racial central, criando um discurso que considerou o negro e seus descendentes, a maioria da população brasileira, nossos criminosos natos e obstáculos ao desenvolvimento e progresso nacional. Neste sentido, Nina Rodrigues esboçou um modelo de controle racial projetado sob um paradigma original que atendia as necessidades de ordem da sociedade brasileira deslegitimando o discurso teórico liberal em relação aos ?inferiores? que deveria ser restrito aos ?superiores?, a raça branca e ariana, defendendo um apartheid brasileiro estribado na cientificidade racial central ao mesmo tempo em que endossava e desvelava as práticas punitivistas escravagistas responsáveis pela contenção do caos ao perseguir os negros em sua ?liberdade?.Resumen : Este trabajo tiene como objetivo principal entender que razón y cómo operalizou la "traducción" (proceso complejo que incluye varias técnicas, alejándose, así, la simple recepción) de la teoría lombrosiana y sobre todo el paradigma establecido por la Criminología Positivista, funcional para el control social en el Centro (Europa) ocupado por Raimundo Nina Rodrigues al control racial en el margen (Brasil) en el post-abolición. En su contexto original, el paradigma tiene sus raíces en la obra L'Uomo Delincuente, que permitió la selección y segregación de una minoría "anormal" en busca de su disciplina a través de los establecimientos penitenciarios, pero su concepción se encuentra en la primera obra de Lombroso, bianco L'uomo y l'uomo do colores: letture sull origine e la varietà delle razze umane, cuya traducción literal sin precedentes se requiere para la comprensión deseada, buscando, además de rescatar a su racismo negada, marque las continuidades y rupturas (si las hay) para la travesía del Atlántico. En este camino, develamos la construcción de su "otro" que nos lleva a la construcción, mucho más allá del centro, sus especies más primitivas: el negro. Para tener la seguridad de su funcionalidad en un contexto periférico especifico, marcado por un fin reciente del sistema esclavista más grande en el mundo, la teoría del delincuente nato posibilito mantener el orden racial a través del marco teórico liberal de la joven República, reanudó su matriz racista y mejorada impulsado por el médico brasileño, legítimo representante de la clase del esclavo, por el eclecticismo teórico y racial central, la creación de un discurso que considera el negro y sus descendientes, la mayoría de la población, los delincuentes y los obstáculos para el desarrollo y el progreso nacional. En este sentido, Nina Rodrigues dio un modelo de control racial diseñado en un paradigma único que respondía a las necesidades de orden de la sociedad brasileña, deslegitimando el discurso teórico liberal en relación con el "inferior" que debería limitarse a "superior", la raza blanca y aria la defensa de un apartheid brasileño estribado en la cientificidad racial del centro, tiempo que respalda y desvelava prácticas punitivistas esclavagistas responsables para la contención del caos para perseguir los negros en su "libertad"

    Development of EMT/TS Co-simulation Using PowerFactory and PSS/E

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    As the scale and complexity of power systems increase, simulating them in efficient and accurate ways continues tobe a challenge in power systems engineering. Electromagnetic Transient (EMT) and Transient Stability (TS) simulation are the two main dynamic power system simulation methods. To simulate large and complex power systems in sufficient detail without sacrificing execution time, one of the idea is to perform a co-simulation that couples EMT and TS simulator. Although several attempts have been made to couple both simulator, only on rare occasions do these hybrid EMT-TS simulators couple two or more industry-adopted simulation tools.The objective of this thesis is to to develop and study the benefits and limitations of the Electromagnetic Transient – Transient Stability co-simulation based on PowerFactory and PSS/E, both of which are among the most extensively used simulation tools in industry and academia alike. With regards to the objective, the EMT-TS co-simulation using PowerFactory and PSS/E has been developed. Then, several tests are performed to evaluate the function of each composing part of the EMT-TS co-simulation, and to test the integration between all its component. Next, The developed co-simulation is applied to study cases and the results are compared to a monolithic EMT simulation to evaluate its accuracy and execution time. Furthermore, the effect of TS and EMT time step to the accuracy and execution time of EMT-TS co-simulation have also been investigated.The study case results show that the developed EMT-TS co-simulation has not been beneficial yet in terms of accuracy and execution time. Although the active power result shows a similar tendency with the monolithic EMT result, the difference between both are visible. The difference between both are more prominent in the reactive power result. The total execution time of the developed co-simulation in the study cases are in the range of 23-24 minutes, significantly larger than the total execution time obtained from the monolithic EMT simulation which is around of 12s. Also, it is found that reducing the TS time step from 0.02 s to 0.01 s slightly increases the total simulation time from 23 to 26 minutes. However, it does not contribute a significant improvement on the accuracy of the developed EMT-TS co-simulation. The result obtained from reducing the EMT time step to 25μs 25 \mu s is the same with the result obtained using 50μs 50 \mu s EMT time step. Moreover, the reduction of the EMT time step significantly increases the total simulation time from 23 to 42 minutes. The developed co-simulation still has a lot of room for improvement and further developments in this topic might increase its performance.Electrical Engineering | Sustainable Energy Technolog

    TS-MPC for autonomous vehicles Including a TS-MHE-UIO estimator

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    © 2019 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.In this paper, a novel approach is presented to solve the trajectory tracking problem for autonomous vehicles. This approach is based on the use of a cascade control where the external loop solves the position control using a novel Takagi Sugeno-Model Predictive Control (TS-MPC) approach and the internal loop is in charge of the dynamic control of the vehicle using a Takagi Sugeno-Linear Quadratic Regulator technique designed via Linear Matrix Inequalities (TS-LMI-LQR). Both techniques use a TS representation of the kinematic and dynamic models of the vehicle. In addition, a novel Takagi-Sugeno estimator-Moving Horizon Estimator-Unknown Input Observer (TS-MHE-UIO) is presented. This method estimates the dynamic states of the vehicle optimally as well as the force of friction acting on the vehicle that is used to reduce the control efforts. The innovative contribution of the TS-MPC and TS-MHE-UIO techniques is that using the TS model formulation of the vehicle allows us to solve the nonlinear problem as if it were linear, reducing computation times by 10-20 times. To demonstrate the potential of the TS-MPC, we propose a comparison between three methods of solving the kinematic control problem: Using the nonlinear MPC formulation (NL-MPC) with compensated friction force, the TS-MPC approach with compensated friction force, and TS-MPC without compensated friction force.This work was supported by the Spanish Min-istry of Economy and Competitiveness (MINECO) and FEDER through theProjects SCAV (ref. DPI2017-88403-R) and HARCRICS (ref. DPI2014-58104-R). The corresponding author, Eugenio Alcalá, is supported under FI AGAURGrant (ref 2017 FI B00433).Peer ReviewedPostprint (author's final draft

    PENERAPAN MODEL PEMBELAJARAN KOOPERATIF TIPE TWO STAY TWO STRAY (TS-TS) TERHADAP KEMAMPUAN KOMUNIKASI MATEMATIKA SISWA

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    The problem that the author encountered in class X SMK Palapa Bandar Lampung is the low ability of mathematical communication. The purpose of the study was to determine the difference in average mathematical communication skills of students who use the Two Stay Two Stray (TS-TS)cooperative learning model with TS-TS using the Conventional learning model in class X students even semester of SMK Palapa Bandar Lampung. This study used an experimental method, the population of the study was 123 students of SMK Palapa Bandar Lampung, samples taken using the Random Sampling Cluster technique, namely the X PM class as the Experiment class and the AP X Class as the Control class. Students\u27 mathematical communication skills are carried out with an essay test which amounts to 6 questions which are first tested for their validity and reliability. From the results of testing the hypothesis using the t-test obtained thit = 4.94 from the t distribution table at a significant level of 5% known tdaf = 1.67. So thit &gt; tdaf so that it can be concluded that "There is an Effect of the Application of the Two Stay Two Stray Cooperative Learning Model (TS-TS) on the Mathematical Communication Ability of Class X Students in the even semester of SMK Palapa Bandar Lampun

    Transition and wavy walls: an experimental study: An experimental study

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    A wide body of research exists which explores the effects of surface roughness or patterned wall shapes on instability growth and transition. Building on those works as well as recent experiments demonstrating passive laminar flow control using arrays of discrete roughness [3, 8], a set of spanwise-wavy walls is designed with the goal of suppressing instability growth in two-dimensional boundary layers. In a numerical investigation of Tollmien-Schlichting (TS) wave growth in the presence of streamwise boundary-layer streaks, Cossu and Brandt [1] found that stabilization of TS waves results from spanwise shear in the mean flow, which forms a negative contribution to production in the perturbation kinetic energy equation. Whereas previous efforts have employed streamwise vorticity developing in roughness wakes to provide the requisite mean-flow deformation, in this work stabilization is achieved through modulation of the no-slip surface. Miniature vortex generators (MVGs) have proven an effective means of producing streamwise streaks for transition delay [8], though relatively large streak amplitudes are necessary to counter their eventual decay through viscous dissipation. The notion motivating this work is that spanwise-wavy walls extended in the streamwise direction can produce a similar effect while avoiding bypass transition resulting from large-amplitude streamwise streaks. Toward that end, six wavy walls are used in a modular test model. When TS waves are excited upstream of the wavy walls, substantial delays in the onset of transition are observed for certain spanwise wavelengths compared with the flat-plate reference case
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