1,720,973 research outputs found
Annual Growth and Phenology of Kyllinga brevifolia (Rottb.) Hassk.in Temperate and Tropical Regions 【Article】
Full text linkBiomass and inflorescence number of Kyllinga brevifoliawere surveyed in Hiroshima Prefecture,Japan (34°18' to 25' N, 132°26' to 45' E) and in Malang, Indonesia (7°44' to 8°26' S, 112°12' to 57'E) to gain understanding of its survivorship in temperate and tropical regions. Monthly observationswere conducted by using 1 m2plots of six study sites in Hiroshima from April 2000 to April 2001 whileobservations in Malang were carried out in ten study sites four times a year from March 2000 to March2001. Considering to developmental stages in the temperate zone, the growing period of K. brevifolia started in the early of April as the beginning of bud sprouting and vegetative development. The genera-tive organ started to appear in the end of May and the first matured seeds occurred in the last week ofJune. The growing period of K. brevifoliaunderwent until December 2000 with a peak in August. Theinflorescences appeared from May to December where its maximum number was in October. Averagedaily temperature was significant correlated with the biomass pattern (r2=0.792). On the other hand, inthe tropical region this plant grew and flowered throughout the year with peaks of biomass and inflores-cence number were in the end of the rainy season (March 2000/2001). The inflorescence number wasminimum in the dry season (July 2000) while the lowest biomass occurred in the early rainy season(November 2000). Furthermore, the pattern of average daily precipitation appeared to be most similarwith the flowering pattern. These diverse patterns of productivity and flowering of K. brevifoliamayexplain a great adaptation of this weed to different climate across the regions
Perception and appreciation of tenggerese of medicinal plants in Wonokitri Village, Tosari subdistrict, Pasuruan Regency
No full textAnnual Growth and Phenology of Kyllinga brevifolia (Rottb.) Hassk.in Temperate and Tropical Regions 【Article】
No full textInventory of Medicinal Plants in Kampung Adat Urug, Urug Village, Sukajaya District, Bogor Regency
No full textSumber Glukomanan Dari Edible Araceae Di Jawa Timur
No full textSumber glukomanan dari edible Araceae terdapat pada genus Amorphophallus, Colocasia, Xanthosoma, Alocasia. Eksplorasi mengenai famili Araceae mencakup berbagai macam tumbuhan Monokotil dengan ciri khas bunga majemuk bertipe "tongkol" yang berseludang (spatha). Araceae merupakan salah satu famili tanaman yang bermanfaat sebagai sumber makanan karena memiliki umbi yang mengandung karbohidrat, protein, glukomanan. Tujuan pelaksanaan penelitian ini adalah untuk mendiskripsikan jenis dari famili Araceae di Jawa Timur yang berpotensi sebagai sumber glukomanan dan mengukur kadar glukomanannya. Pengambilan sampel Araceae di wilayah Jawa Timur dikategorikan menjadi 4 (empat) area geografis, yaitu: a. wilayah selatan dan tengah Jawa Timur (Blitar, Malang, Lumajang); b. wilayah timur Jawa Timur (Banyuwangi, Situbondo, Bondowoso); c. wilayah barat Jawa Timur (Madiun, Nganjuk, Tuban); d. wilayah pulau Madura (Bangkalan, Sampang, Pamekasan). Pada tiap studi area diambil minimal tiga tanaman untuk masing-masing sampel spesies dari famili Araceae secara acak, untuk diamati dan dideskripsikan keseluruhan bagian tanaman tersebut. Pengamatan morfologi dilakukan visual maupun dengan alat bantu kaca pembesar dan mikroskop. Kadar glukomanan dianalisis setelah sentrifugasi ektrak umbi. Hasil pengamatan/pengukuran itu disusun dalam suatu tabel determinasi. Dari kunci tabel ini akan terlihat adanya perbedaan morfologi dan kadar glukomanan dari anggota famili Araceae yang ditemukan. Diperoleh 12 jenis dari empat genus Araceae di 4 area geografis di Jawa Timur yang mengandung glukomanan. Kadar glukomanan sebagai berikut: tertinggi Amorphophallus muelleri Bl. (porang) 9,92 % (berat basah), A.paeoniifolius (suweg) 3,2 % (bb), ), A. variabilis Blume. (walur) 2,52 % (bb), Colocasia esculenta (L.) Schott. (bentul) 2,4 % (bb), Alocasia macrorrhiza (L.) Schott. (sente) 1,3 % (bb) dan terendah Xanthosoma sp.(endro/mbote kuning) 0,64 % (bb).
Kata kunci: Araceae, Sumber Glukomanan, Jawa Timu
Bacteriocin production of Lactobacillus sp. from intestines of ducks (Anas domesticus L.) incubated at room temperature and antibacterial effectivity against pathogen
Full text linkBacteriocin is a peptide that is easily degraded by proteolytic enzymes in the digestive systems of animals,including humans. It has antimicrobial activity against pathogenic bacteria. Lactobacillus sp. is one type of lactic acid bacteria (LAB) that occupies the intestines of ducks (Anas domesticus L.). The purpose of this research was to determine the optimum time of the highest protein production by Lactobacillus sp. and to determine inhibitory activity of bacteriocin
against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Using the Bradford method, the results showed that the optimum time of highest bacteriocin production was after 36 hours of incubation, with a protein content of 0.93 mg/ml. The bacteriocin inhibitory activity against Escherichia coli showed that a protein concentration of 30% gave a maximum inhibition index of 1.1 mm, while for Staphylococcus aureus, a concentration of 70% gave a maximum inhibition
index of 0.3 mm. Further research is required to determine the stationary state of bacteriocin production in this
circumstance
The Analysis of Pandanus Relationship of Purwodadi Botanical Garden Collections Based on Morphological Character and Molecular Marker (trnL and trnL-F)
No full textThe morphological characteristics of plant vegetative organ has the potential to be the defining factor of pandanus family collection at Purwodadi Botanical Garden (PBG). However, the information is not enough to confirm the identity of pandanus at the type-level. This problem happened at the collection of PBG. It is an urgent issue considering PBG as one of the most important ex-situ plant conservation institute that also has function as a study source for plant taxonomy science in Indonesia. Therefore, the use of molecular technology analysis using molecular marker (primer) is incredibly important to identify the member of pandanus family by identifying its family relationship pattern. This information was expected to give a clarity about the identity of Pandanus family at PBG which will support further research and activity for pandanus conservation. The plant sample includes 16 specimens of pandanus with one member of the family from Freycinetia as the outgroup. Morphological analysis of the 50 characteristics of vegetative organs (leaf, stem, and root) was conducted. Molecular analysis of DNA using primer of trnL and trnL-F. The data sequence as a result of the analysis was then tested using homology test with BLAST. All of the DNA sequence was paralleled using Clustal W analysis, the tree construction relationship was analysed using Maximum Parsimony (MP) method with MEGA 6.0 program. The results of morphological studies showed the character vegetative that is the position of the spines on the leaves, the shape of apex leaf and the condition of the root surface above the soil. Molecularly, appears 1 new genera that has not been previously identified by PBG researcher is Benstonea
Proteasome and the Deleted-in-Split Hand/Split Foot 1 Protein SPDSS1 Participate in the Modulation of Drought Stress Tolerance in Sweet Potato Leaves
Full text linkIn recent year drought and soil salinization of cultivated lands under extreme climate severely result in the unbalance between agricultural crop production and demand-provision systems. Therefore, improvement of crop stress tolerance especially for drought is an important world-wide issue. Ubiquitin-Proteasome System (UPS) widely exists in plants and plays a role in association with abiotic salt stress tolerance in sweet potato. However, its physiological function under drought stress is unclear. In sweet potato, drought promoted senescence and necrosis in leaves. Significant morphological, biochemical and metabolic changes of senescence-associated markers, including morphological yellowing/necrosis, reduction of chlorophyll and Fv/Fm contents, decrease of antioxidant enzyme/activity levels, elevation of NO/H2O2/MDA amounts, increase of mitogen-activated protein kinase/phosphorylation levels, and enhancement of cysteine protease SPCP1 expression were observed in treated leaves on days 12 and 15 as compared to the untreated control. Drought-mediated effects were all alleviated by the proteasome inhibitor MG132, suggesting an association of proteasome activities in drought stress tolerance. In our laboratory, a cloned full-length cDNA SPDSS1 from sweet potato leaves encodes a putative deleted in split hand/split foot 1 (DSS1)-like protein, which is an acid protein with 75 amino acids (228 nucleotides) and three conserved aspartic/glutamic acid-rich domains in its open reading frame and is a candidate subunit of the 19S regulatory proteasome according to the previous report in Arabidopsis. Drought enhanced SPDSS1 expression level earlier than the induced leaf senescence. Exogenous SPDSS1 fusion protein accelerated drought-induced senescence/necrosis and enhanced changes of senescence-associated markers on day 9 and 12 in treated leaves, which could be inhibit and reversed by the proteasome inhibitor MG132. Based on these results we conclude that proteasome inhibitor MG132 and the 19S regulatory proteasome subunit deleted in split hand/split foot 1 (DSS1)-like protein SPDSS1 participate in the modulation of drought stress tolerance in sweet potato leaves. A novel physiological role of the Ubiquitin-Proteasome system in association with drought stress tolerance is also suggested
Taxonomical position of Annonaceae species from East Java, Indonesia: Collections of Purwodadi Botanic Garden based on morphological character
No full textLestari DA, Azrianingsih R, Hendrian H. 2017. Taxonomical position of Annonaceae species from East Java, Indonesia: collections of Purwodadi Botanic Garden based on morphological character. Biodiversitas 18: 1067-1076. Morphological characters are one of the most consistent of taxonomical markers are used in Annonaceae today. One of the plants collections from Annonaceae species which came from East Java and conserved in Purwodadi Botanic Garden-Indonesian Institute of Sciences, Purwodadi, and East Java has some problems in morphological characters. It has some problems that causing the taxonomical position of these species are not clear, i.e. presence of unidentified plant until level species. The aim of this study is to classify and identify of species that have not been identified in order to taxonomical position be clearly. Material samples are used Annonaceae collection from East Java and Magnoliaceae as out-group. Samples of these plants were observed qualitatively and quantitatively through morphological characters were analyzed using cluster analysis and then synapomorphy, autapomorphy and apomorphy character analysis to identify and to determine the taxonomical position. The results showed that there are 4 tribes and two sub-families in the dendrogram. Tribe includes Miliuseae, Xylopiae, Annoneae and Uvariae, as well as sub-family of Malmeoideae and Annonoideae. Sub-family distinguished by inner petal and habit, sub-family of Malmeoideae has connate inner petals and trees habit, sub-family of Annonoideae has free inner petal and woody climber habit, some of the trees. Species are unidentified suspected as a different species based on the proximity of group formed. There are five plant species that have not been identified to the species level. These species can be recommended the taxonomical position and proposed of species name through dendrogram with the name of the specimen. Specimen 1 is predicted to have close relations with Annona muricata, specimen 2 is predicted to have close relations with Miliusa macropoda, specimen 3 is predicted to have close relations with Artabotrys uncinatus and identified as Uvaria micrantha, specimen 4 is predicted to have close relations with Fissistigma latifolium and specimen 5 is predicted to have close relations with Saccopetalum horsfieldii, because these species are in one group.</jats:p
Identification of potential pathogen fungi which cause rotten on Porang (Amorphophallus muelleri Blume) tubers
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