12,117 research outputs found
Dynamics and folding of single two-stranded coiled-coil peptides studied by fluorescent energy transfer confocal microscopy
No full textWe report single-molecule measurements on the folding and unfolding conformational equilibrium distributions and dynamics of a disulfide crosslinked version of the two-stranded coiled coil from GCN4. The peptide has a fluorescent donor and acceptor at the N termini of its two chains and a Cys disulfide near its C terminus. Thus, folding brings the two N termini of the two chains close together, resulting in an enhancement of fluorescent resonant energy transfer. End-to-end distance distributions have thus been characterized under conditions where the peptide is nearly fully folded (0 M urea), unfolded (7.4 M urea), and in dynamic exchange between folded and unfolded states (3.0 M urea). The distributions have been compared for the peptide freely diffusing in solution and deposited onto aminopropyl silanized glass. As the urea concentration is increased, the mean end-to-end distance shifts to longer distances both in free solution and on the modified surface. The widths of these distributions indicate that the molecules are undergoing millisecond conformational fluctuations. Under all three conditions, these fluctuations gave nonexponential correlations on 1- to 100-ms time scale. A component of the correlation decay that was sensitive to the concentration of urea corresponded to that measured by bulk relaxation kinetics. Thetrajectories provided effective intramolecular diffusion coefficients as a function of the end-to-end distances for the folded and unfolded states. Single-molecule folding studies provide information concerning the distributions of conformational states in the folded, unfolded, and dynamically interconverting states.Author manuscript. Published in final edited form as: Proc Natl Acad Sci U S A. 2000 November 21; 97(24): 13021-13026.The final published version of this article is located at: http://www.pnas.org/cgi/reprint/97/24/13021NIH GM54616; to William F. DeGradoNIH GM12592; to Robin M. HochstrasserNIH GM48130; to William F. Degrado and Robin M. HochstrasserThis work was supported by GM54616 (to W.F.D.), GM12592 (to R.M.H.) and GM48130 (to W.F.D. and R.M.H.) with instrumentation developed under RR01348. D.S.T. was supported by National Institutes of Health Grant NRSA F32-GM18589.Also available in PubMed Central. PMCID:PMC2717
Development of Indicators to Assess Quality of Care for Prostate Cancer
No full textAbstract not availableNupur Nag, Jeremy Millar, Ian D. Davis, Shaun Costello, James B. Duthie, Stephen Mark, Warick Delprado, David Smith, David Pryor, David Galvin, Frank Sullivan, Aine C. Murphy, David Roder, Hany Elsaleh, David Currow, Craig White, Marketa Skala, Kim L. Moretti, Tony Walker, Paolo De Ieso, Andrew Brooks, Peter Heathcote, Mark Frydenberg, Jeffery Thavaseelan, Sue M. Evan
Leveraging diversity in American academic medicine. The Harold Amos Medical Faculty Development Program
No full textPeer reviewe
Landsat MSS classification of fire fuel types in Wood Buffalo National Park, northern Canada
No full textJ1: Global Ecology & Biogeography Letters; M3: Article; Milne, David Franklin, Steven E. Wilson, Bradley A. Ghitter, Geoff Heathcott, Mark McCaffrey, Thomas M. Ow, Charlotte F. Y.; Source Information: Mar1994, Vol. 4 Issue 2, p33; Subject Term: FOREST fires; Author-Supplied Keyword: Canada (Wood Buffalo National Park); Author-Supplied Keyword: Forest fire; Author-Supplied Keyword: Fuel type classification; Author-Supplied Keyword: Landsat data; Number of Pages: 0p; Document Type: Articl
Disparities in cancer stage at diagnosis and survival of Aboriginal and non-Aboriginal South Australians
No full textData source: Supplementary data, http://www.sciencedirect.com/science/MiamiMultiMediaURL/1-s2.0-S1877782117300590/1-s2.0-S1877782117300590-mmc1.docx/277953/html/S1877782117300590/f04af5626552e7f327193492ef88fcc4/mmc1.docxAbstract not availableDavid Banham, David Roder, Dorothy Keefe, Gelareh Farshid, Marion Eckert, Margaret Cargo, Alex Brown, for the CanDAD Aboriginal Community Reference Group and other CanDAD investigator
TRAUMATIC IDENTITY AND AURA IN DAVID LODGE’S AUTHOR, AUTHOR
No full textThis paper delves into David Lodge’s Author, Author (2004) as an example of neo-Victorian celebrity biofiction, more concretely on Henry James. The genre belongs to the wave of Victorian revival in current literature which also affects cultural studies in general. My main contention is that Lodge’s novel responds to current cultural anxieties, particularly the crisis of identity and authorship and the end of Walter Benjamin’s concept of aura, by sublimating them into late-nineteenth-century traumata. The choice of James is, the article argues, not casual. He represents the redeeming figure of a lost auratic world; the human in crisis, traumatized because he does not fit in the new status quo.Este artículo analiza la novela Author, Author (2004) de David Lodge como ejemplo de bioficción neo-victoriana centrada en una celebridad, en este caso concreto, Henry James. El género forma parte del renacimiento victoriano actual que afecta a los estudios culturales en su conjunto. Mi argumento central es que la novela de Lodge constituye una respuesta a las ansiedades culturales actuales, en particular a las que se refieren a la crisis identitaria y autoría literaria, así como a la pérdida del aura artística de Walter Benjamin, sublimándolas a través de los traumas de finales del siglo XIX. La elección de James, como demuestra el artículo, no es casual. Es el último representante de un mundo perdido en el que el aura aún tenía un espacio; el ser humano en crisis y traumatizado porque no encaja en un status quo nuevo
Distinguished Lecture Series 2011: David M. Oshinsky, Pulitzer Prize-winning author
No full textDavid M. Oshinsky, Pulitzer Prize-winning author and History professor at The University of Texas at Austin, will be the speaker for the fall Distinguished Lecture Series at The University of Texas at Brownsville and Texas Southmost College. Oshinsky won the Pulitzer Prize in History for his book, Polio: An American Story. He will speak at 7 p.m., Thursday, October 13, at the Science and Engineering Technology Building Lecture Hall.https://scholarworks.utrgv.edu/utbmedia/1102/thumbnail.jp
Patient and carer perceptions of cancer care in South Australia
No full textLink to a related website: http://www.publish.csiro.au/ah/pdf/ah090645, Open Access via UnpaywallQuality of care from the patient's perspective is an increasingly important outcome measure for cancer services. Patients' and carers' perceptions of cancer care were assessed through structured telephone interviews, 4-10 months post-discharge, which focused on experiences during the most recent hospital admission. A total of 481 patients with a primary diagnosis of cancer (ICD-10 C codes) were recruited, along with 345 carers nominated by the patients. Perceptions of clinical care were generally positive. Less positive aspects of care included not being asked how they were coping, not being offered counselling, and not receiving written information about procedures. Results also highlighted inadequate discharge processes. Carers were more likely than patients to report negative experiences. Perceptions of care also differed by cancer type.Kerri R. Beckmann, Ian N. Olver, Graeme P. Young, David M. Roder, Linda M. Foreman and Brenda Wilso
Issues of consent and privacy affecting the functioning of ASERNIP-S
No full textThe definitive version is available at www.blackwell-synergy.comThe Australian Safety and Efficacy Register for New Interventional Procedures − Surgical (ASERNIP−S) undertakes horizon scanning, systematic reviews and audits. By disseminating information derived from these processes, ASERNIP−S aims to improve the quality of health care. In the present article, we discuss some of the legal and ethical issues surrounding the collection of identified data for the purposes of audit. The individual's right to privacy is considered as well as the benefits of improving the quality of surgical health care.Maggi Boult, Wendy Babidge, David Roder and Guy Madder
Simulation of thermal plant optimization and hydraulic aspects of thermal distribution loops for large campuses
No full textFollowing an introduction, the author describes Texas A&M University and its utilities system. After that, the author presents how to construct simulation models for chilled water and heating hot water distribution systems. The simulation model was used in a $2.3 million Ross Street chilled water pipe replacement project at Texas A&M University. A second project conducted at the University of Texas at San Antonio was used as an example to demonstrate how to identify and design an optimal distribution system by using a simulation model. The author found that the minor losses of these closed loop thermal distribution systems are significantly higher than potable water distribution systems. In the second part of the report, the author presents the latest development of software called the Plant Optimization Program, which can simulate cogeneration plant operation, estimate its operation cost and provide optimized operation suggestions. The author also developed detailed simulation models for a gas turbine and heat recovery steam generator and identified significant potential savings. Finally, the author also used a steam turbine as an example to present a multi-regression method on constructing simulation models by using basic statistics and optimization algorithms. This report presents a survey of the author??s working experience at the Energy Systems Laboratory (ESL) at Texas A&M University during the period of January 2002 through March 2004. The purpose of the above work was to allow the author to become familiar with the practice of engineering. The result is that the author knows how to complete a project from start to finish and understands how both technical and nontechnical aspects of a project need to be considered in order to ensure a quality deliverable and bring a project to successful completion. This report concludes that the objectives of the internship were successfully accomplished and that the requirements for the degree of Degree of Engineering have been satisfied
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