128,760 research outputs found
Flow-assisted analysis
No full textThe demand for direct, robust and quantitative determination of analytes even in complex matrices of different origin produced an increase interest in flow-assisted techniques for automatization of chemical analysis with high throughput and reproducibility. With this aim, thanks to simple instrumentation, high sensitivity and low background signal, chemiluminescence, bioluminescence and electrochemiluminescence represent suitable detection techniques to which flow-analysis can be coupled. In this Chapter the fundamental, instrumental setup and recent application are reviewed for flow-injection analysis and its variants, and for field-flow fractionation, a flow-assisted technique suitable to separate analytes with high molar mass, for the development of assays with reliability, rapidity and robustness. A discussion on the miniaturization of these approaches to add features such as low reagent consumption and reduced analysis time is also presented
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Shanghai, tra Cina e Mondo. Eccezione e regola di una città esemplare
No full textC’è un luogo a Shanghai dove lo spaesamento
per le dimensioni della metropoli
si accompagna ad una presa di coscienza
– impattante e quasi fisica – delle questioni
dello sviluppo urbano contemporaneo,
che si applica alle grandi conurbazioni del
Sud-Est asiatico e che ha proprio qui uno
dei suoi casi più eclatanti: Shanghai è emblema
di quel fenomeno che Cesare De
Seta (anche nel suo recente volume edito
da Rizzoli La città. Da Babilonia alla Smart
City) definisce global city.There is a place in Shanghai where the
disorientation for the metropolis’ huge
dimensions comes with the awareness –
impacting and almost physical – of the
issues of contemporary urban development.
It’s a feeling you can apply to the
large conurbations of South-east Asia and
it has one of its most striking study-cases
here: Shanghai is the emblem of the phenomenon
that an important critic of the
cities, as Cesare De Seta (also in his recent
book published by Rizzoli La città. Da Babilonia
alla Smart City), calls global city
Shanghai, between China and the World. Exception and rule of an exemplary city / Shanghai, tra Cina e Mondo. Eccezione e regola di una città esemplare
No full textDispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Bigamiam, h. e. Geminam Unius Desponsationem in Nuptiis Baudisio-Ettmüllerianis, die XX. Octobr. M.DC.LXXXV. celebrandis exponit, & in peretuum Observantiae testimonium votiva sua acclamatione prosequitur W. F. a Roda, B. T.
No full textBIGAMIAM, H. E. GEMINAM UNIUS DESPONSATIONEM IN NUPTIIS BAUDISIO-ETTMÜLLERIANIS, DIE XX. OCTOBR. M.DC.LXXXV. CELEBRANDIS EXPONIT, & IN PERETUUM OBSERVANTIAE TESTIMONIUM VOTIVA SUA ACCLAMATIONE PROSEQUITUR W. F. A RODA, B. T.
Bigamiam, h. e. Geminam Unius Desponsationem in Nuptiis Baudisio-Ettmüllerianis, die XX. Octobr. M.DC.LXXXV. celebrandis exponit, & in peretuum Observantiae testimonium votiva sua acclamatione prosequitur W. F. a Roda, B. T. ([1])
Titelseite ([1])
Text ([1]
Pairwise alignments for all hydrophobin sequences in comparison to RodA.
No full text<p>(<b>A</b>) Up: Hydrophobicity of the alignment of RodA with DewA. In comparison with DewB, DewA does not share as much similarity in the hydrophobicity pattern with RodA. The two hydrophobic unstructured loops are conserved; DewA features a stronger hydrophobic region towards the N-Terminus. Middle: Predicted disorder for RodA/DewA. Similar to RodA, intrinsically disordered regions are predicted for two large loops towards the C-Termini. (<b>B</b>) Up: Hydrophobicity of the alignment of RodA with DewB. Due to their similarity in sequence RodA and DewB share similar hydropathicity patterns. A large gap is observed in the sequence of DewB towards the N-Terminus. Middle: Predicted disorder for RodA/DewB. Both proteins share a very similar predicted disorder pattern. The RodA sequence aligned to the gap in DewB is predicted to be largely unstructured. The two unstructured loops between the cysteines towards the C-Terminus can be clearly discerned. (<b>C</b>) Up: Hydrophobicity of the alignment of RodA with DewC. Especially towards the C-Terminus DewC exhibits a hydrophobicity pattern unlike the one from RodA. Middle: Predicted disorder for RodA/DewC. A large unordered region was predicted for the DewC sequence. Like all other analyzed hydrophobins except for DewD, the large unstructured loop (alignment positions 100-150) is conserved. (<b>D</b>) Up: Hydrophobicity of the alignment of RodA with DewD. DewD exhibits a hydrophobicity pattern unlike all the other hydrophobins. Especially the characteristic loop between alignment positions 125 and 150 is missing. Middle: Predicted disorder for RodA/DewD. DewD is predicted to exhibit a very high amount of intrinsic disorder. (<b>E</b>) Up: Hydrophobicity of the alignment of RodA with DewE. The hydrophobicity pattern of DewE is unlike that of RodA with a second hydrophobic region in the middle of the sequence. Middle: Predicted disorder for RodA/DewE. The two short unordered loops of DewE are shifted in comparison with RodA. For all images: Down: Sequence Alignment Blue/Red RodA/Gaps, Green/Yellow Other/Gaps</p
Compact miniaturized bioluminescence sensor based on continuous air-segmented flow for real-time monitoring: Application to bile salt hydrolase (bsh) activity and atp detection in biological fluids
No full textA simple and versatile continuous air-segmented flow sensor using immobilized luciferase was designed as a general miniaturized platform based on sensitive biochemiluminescence detection. The device uses miniaturized microperistaltic pumps to deliver flows and compact sensitive light imaging detectors based on BI-CMOS (smartphone camera) or CCD technology. The low-cost components and power supply make it suitable as out-lab device at point of need to monitor kinetic-related processes or ex vivo dynamic events. A nylon6 flat spiral carrying immobilized luciferase was placed in front of the detector in lensless mode using a fiber optic tapered faceplate. ATP was measured in samples collected by microdialysis from rat brain with detecting levels as low as 0.4 fmoles. The same immobilized luciferase was also used for the evaluation of bile salt hydrolase (BSH) activity in intestinal microbiota. An aminoluciferin was conjugatated with chenodeoxycholic acid forming the amide derivative aLuc-CDCA. The hydrolysis of the aLuc-CDCA probe by BSH releases free uncaged aminoluciferin which is the active substrate for luciferase leading to light emission. This method can detect as low as 0.5 mM of aLuc-CDCA, so it can be used on real faecal human samples to study BSH activity and its modulation by diseases and drugs
Almond oxynitrilase.catalyzed transformation of substituted aldehydes. Part 2
No full textDifferent α- and β-alkyl substituted aldehydes (1-5) have been submitted to the catalytic action of almond oxynitrilase, in order to explore the influence of a stereocenter already present in the substrate molecule on the selectivity of this enzyme. The results indicate that substituents in α- position to the aldehyde group significantly influence the stereochemical outcome of the AON-catalyzed transformation, especially when they are of different size. On the other hand, the presence of a β-substituent seems to have little effect on AON selectivity
Field-flow fractionation and biotechnology
No full textThe gentle separation mechanism has made field-flow
fractionation particularly suited to samples of biotechnological
interest, from proteins and nucleic acids to
viruses, subcellular units and whole cells. Recent
progress in field-flow fractionation technology, as well
as the development of coupled techniques combining
field-flow fractionation capabilities with the specificity
and sensitivity of well-established analytical methods,
opens up new biotechnological applications for fieldflow
fractionation. The most recent appealing applications
include: sorting and fingerprinting of bacteria for
whole-cell vaccine production; noninvasive and tagless
sorting of immature and stem cells; separation of intact
proteins and enzymes in top-down proteomics; and the
development of flow-assisted, multianalyte immunoassays
using nano- and micron-sized particles with
immobilized biomolecules
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