1,721,003 research outputs found
Proteomic approaches by SELDI and MALDI-TOF/MS for CTL analysis
No full textExtracellular stimuli activate, on target cells, a number of signal transduction processes regulating gene expression and the function and/or synthesis of the proteins. In order to highlight the slight changes of the quantity and quality of the proteome it is essential to optimize preparative strategies able to improve the signals of the less expressed proteins and to standardize the use of high-throughput techniques useful to detect them. We describe a complete workflow useful to enrich, from PBMC protein extracts and extrapolated to their subpopulations, the low-molecular-weight proteins and peptides and to detect them by SELDI-TOF protein profiling. The described protocol can also be applied to MALDI-TOF/MS instruments in order to obtain fast, reproducible, and high-quality protein profiles
Apigenin: Molecular Mechanisms and Therapeutic Potential against Cancer Spreading
Full text linkDue to its propensity to metastasize, cancer remains one of the leading causes of death worldwide. Thanks in part to their intrinsic low cytotoxicity, the effects of the flavonoid family in the prevention and treatment of various human cancers, both in vitro and in vivo, have received increasing attention in recent years. It is well documented that Apigenin (4 ',5,7-trihydroxyflavone), among other flavonoids, is able to modulate key signaling molecules involved in the initiation of cancer cell proliferation, invasion, and metastasis, including JAK/STAT, PI3K/Akt/mTOR, MAPK/ERK, NF-kappa B, and Wnt/beta-catenin pathways, as well as the oncogenic non-coding RNA network. Based on these premises, the aim of this review is to emphasize some of the key events through which Apigenin suppresses cancer proliferation, focusing specifically on its ability to target key molecular pathways involved in angiogenesis, epithelial-to-mesenchymal transition (EMT), maintenance of cancer stem cells (CSCs), cell cycle arrest, and cancer cell death
Novel syntheses of 5-acetyl-2,3-dihydro-1,4-thiazine, a very intense roasty, popcorn-like odorant.
No full textDeprotonation of 3-Chloropropenyl-2-Oxazolines: Regio- and Stereochemistry of Reactions with Electrophiles
No full textMetalation of 2-chloromethyl-2-oxazolines: Synthesis of 1,2,3-tris(oxazolinyl)cyclopropanes and derivatives
No full text2-Chloromethyl-2-oxazoline converts cleanly into trans-1,2,3-tris(oxazolinyl)cyclopropane upon treatment with strong bases such as LDA or KN(SiMe3)2. Deprotonation of the above cyclopropane followed by the addition of electrophiles allows the preparation of more functionalized tris-(oxazolinyl)cyclopropanes
New general synthesis of α-alkoxyketones via α′- alkylation, α-alkylation and α,α′-dialkylation of α-alkoxyketimines
No full textα-Methoxy- and α-ethoxyketones, as important intermediates in organic synthesis and flavor compounds in food chemistry, were synthesized by deprotonation of N-(1-alkoxy-2-propylidene)isopropylamine, prepared by condensation of the corresponding α-alkoxyacetone with isopropylamine, and subsequent reaction of the corresponding 1-azaallylic anions with alkyl halides to afford α′-alkylated, α-alkylated and α, α′-dialkylated ketimines. Hydrolysis of the imino function led to the desired substituted α-alkoxyketones. The ratio of α-, α′-, and α,α′-(di)alkylated compounds depended on the amount of base used and on the nature of the alkylating reagent. © 2011 The Royal Society of Chemistry
Deprotonazione di Cloroallilossazoline: Regio e Stereochimica delle Reazioni con Elettrofili
No full textMetalation of 2-Chloromethyl-2-oxazolines: Synthesis of 1,2,3-Tris(oxazolinyl)cyclopropanes and Derivatives
No full text2-Chloromethyl-2-oxazoline converts cleanly into trans-1,2,3-tris(oxazolinyl)cyclopropane upon treatment with strong bases such as LDA or KN(SiMe 3) 2. Deprotonation of the above cyclopropane followed by the addition of electrophiles allows the preparation of more functionalized tris-(oxazolinyl)cyclopropanes
Lithiation of 2-Chloromethyloxazolines: Synthesis of Trioxazolinylcyclopropanes and Derivatives
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