52 research outputs found
Biotization of encapsulated in vitro derived propagules of Carrizo Citrange.
No full textThe encapsulation technology represents a new tool to integrate micropropagation into the nursery activity. It allows combining the advantages of zygotic or gamic seeds with those of micropropagation. The synthetic or artificial seeds have been defined as “artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions”. This will be a powerful propagation tool in the nurseryman hands, if the levels of the synthetic seeds conversion can be increased also in the nurseries, without the asepsis of in vitro laboratories and with the presence of many parasitic microorganisms, like bacteria and fungi, responsible for contamination and/or for trophic competition. This research was carried out in order to apply biotization into the synthetic seed technology of Carrizo citrange [C. sinensis (L.) Osb. × Poncirus trifoliate (L.) Raf.], one most extensively used citrus rootstock, because of its resistance to the Citrus tristeza virus (CTV). With this goal, preliminary experiments to set up protocols for biotization, through the introduction of Plant Growth Promoting Bacteria (PGPB) into calcium alginate capsules of Carrizo citrange in vitro-derived microcuttings, were carried out, in order to protect the plantlets from abiotic and biotic factors and to promote their growth during the first stages of development. Specifically, the Sinorhizobium meliloti wild type strain 1021 and its derivative RD64, that synthesizes 80-fold more IAA as compared to the wild type strain, was used to evaluate their performance in inducing rooting of encapsulated microcuttings
Biotization of encapsulated in vitro derived propagules of Carrizo citrange
No full textThe encapsulation technology represents a new tool to integrate micropropagation into the nursery activity. It allows combining the advantages of zygotic or gamic seeds with those of micropropagation. The synthetic or artificial seeds have been defined as "artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions". This will be a powerful propagation tool in the nurseryman hands, if the levels of the synthetic seeds conversion can be increased also in the nurseries, without the asepsis of in vitro laboratories and with the presence of many parasitic microorganisms, like bacteria and fungi, responsible for contamination and/or for trophic competition. This research was carried out in order to apply biotization into the synthetic seed technology of Carrizo citrange [C. sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.], one most extensively used citrus rootstock, because of its resistance to the Citrus tristeza virus (CTV). With this goal, preliminary experiments to set up protocols for biotization, through the introduction of Plant Growth Promoting Bacteria (PGPB) into calcium alginate capsules of Carrizo citrange in vitro-derived microcuttings, were carried out, in order to protect the plantlets from abiotic and biotic factors and to promote their growth during the first stages of development. Specifically, the Sinorhizobium meliloti wild type strain 1021 and its derivative RD64, that synthesizes 80-fold more IAA as compared to the wild type strain, was used to evaluate their performance in inducing rooting of encapsulated microcuttings
A Method to Solve Non-homogeneous Strongly Coupled Mixed Parabolic Boundary Value Systems with Non-homogeneous Boundary Conditions
No full textIn this paper, a method to construct the solution of non-homogeneous
parabolic coupled systems with non-homogeneous boundary conditions
of the type ut−Auxx = G(x, t), A1u(0, t)+B1ux(0, t) = P(t), A2u(l, t)+
B2ux(l, t) = Q(t), 0 0, u(x, 0) = f(x), where A is a positive
stable matrix and A1, A2, B1, B2 are arbitrary matrices for which
the block matrix
A1 B1
A2 B2
is non-singular, is proposed. Two illustrative
examples of the method are given.Soler Basauri, V.; Defez Candel, E.; Capilla Lladró, R. (2015). A Method to Solve Non-homogeneous Strongly Coupled Mixed Parabolic Boundary Value Systems with Non-homogeneous Boundary Conditions. International Journal of Mathematical Analysis. 9(40):1955-1970. doi:10.12988/ijma.2015.57176S1955197094
Bisphenol A removal by a Pseudomonas aeruginosa immobilized on granular activated carbon and operating in a fluidized bed reactor
No full textSerratia rubidiae, Pseudomonas aeruginosa and Escherichia coli K12 have been studied for their ability ofBisphenol A removal from aqueous systems and biofilm formation on activated granule carbon. Math-ematical equations for biodegradation process have been elaborated and discussed. P. aeruginosa wasfound the best strain to be employed in the process of Bisphenol A removal. The yield in BPA removalof a P. aeruginosa biofilm grown on GAC and operating in a fluidized bed reactor has been evaluated.The results confirm the usefulness in using biological activated carbon (BAC process) to remove phenolcompounds from aqueous systems
PRELIMINARY SURVEYS FOR THE GENETIC VARIABILITY OF RICE ASSOCIATION WITH PLANT GROWTH PROMOTING BACTERIA
No full textThe root endophytic microbiota can enhance plant tolerance to biotic an abiotic environmental challenges in several plant species, including th rice crop. It is possible that during plant domestication and varie selection the aptitude of rice roots to associate with be microbiota has been modified, since this aptitude was not a direct target of selection and because the human input for plant nutrition and defenc have lowered the importance of the microbiota contribution for traits. To verify whether there is genetic variability in the level of rice root association with selected endophytic plant growth promoting bacteria (PGPB), we evaluated such aptitude in a range ofOryza sativa ssp. japonica accessions and in a wild ricOeryza( rufipogon) accession. Results highlighted a variability in both the germplasm pools, in particular, the rice variety Baldo and the wild riOc.e rufipogon accession showed an aptitude to associate with a nitrogen fixing endophytic strain at least ten and sixty times, respectively, higher than Vialone Nano. Availability o segregating populations derived from crossing Baldo x Vialone (recombinant inbred lines, RILs) and Vialone Nano xO. rufipogon accession (BC3F4 introgression lines) are allowing preliminary evaluations of t segregation of the association aptitude in these rice genotypes and the identification of lines with enhanced aptitude to associate with PBPG
Improved Drought Stress Response in Alfalfa Plants Nodulated by an IAA Over-producing Rhizobium Strain
No full textThe drought–stress response in plant involves the cross-talk between abscisic acid (ABA) and other phytohormones, such as jasmonates and ethylene. The auxin indole-3-acetic acid (IAA) plays an integral part in plant adaptation to drought stress. Investigation was made to see how the main auxin IAA interacted with other plant hormones under water stress, applied through two different growth conditions (solid and hydroponic). Medicago sativa plants nodulated by the Ensifer meliloti wild type 1021 (Ms-1021) and its IAA-overproducing RD64 derivative strains (Ms-RD64) were subjected to drought stress, comparing their response. When the expression of nifH gene and the activity of the nitrogenase enzyme were measured after stress treatments, Ms-RD64 plants recorded a significantly weaker damage. These results were correlated with a lower biomass reduction, and a higher Rubisco protein level measured for the Ms-RD64-stressed plants as compared to the Ms-1021-stressed ones. It has been verified that the stress response observed for Ms-RD64-stressed plants was related to the production of greater amount of low-molecular-weight osmolytes, such as proline and pinitol, measured in these plants. For the Ms-RD64 plants the immunoblotting analysis of thylakoid membrane proteins showed that some of the photosystem proteins increased after the stress. An increased non-photochemical quenching after the stress was also observed for these plants. The reduced wilting signs observed for these plants were also connected to the significant down-regulation of the MtAA03 gene involved in the ABA biosynthesis, and with the unchanged expression of the two genes (Mt-2g006330 and Mt-8g095330) of ABA signaling. When the expression level of the ethylene-signaling genes was evaluated by qPCR analysis no significant alteration of the key positive regulators was recorded for Ms-RD64-stressed plants. Coherently, these plants accumulated 40% less ethylene as compared to Ms-1021-stressed ones. The results presented herein indicate that the variations in endogenous IAA levels, triggered by the overproduction of rhizobial IAA inside root nodules, positively affected drought stress response in nodulated alfalfa plants
La impureza de la filosofía
No full textThe main purpose of this article is to revise the question of whether or not philosophical truths exist and if so, what is their status, what makes them specific and sets them apart from other truths. Following an analysis of the two tendencies that appear as answers to this question, characterized by the thoughts of Hegel and Schopenhauer, the author argues that there are no philosophical truths and furthermore, claims that philosophy is an impure activity, which cannot be reconciled the empirical and formal disciplines. Thus, the paper concludes that philosophy should not be a servant of any other discipline or activity.El presente artículo supone una revisión de la pregunta de si hay o no verdades filosóficas y, de haberlas, en qué consisten, qué las hace específicas, distintas a otras verdades. Tras un análisis de las dos tendencias que se presentan como respuesta a tal pregunta, caracterizadas por los pensamientos de Hegel y Schopenhauer el autor afirma y argumenta que no hay verdades filosóficas y además que la filosofías es una actividad impura irreductible a las disciplinas empíricas y formales. Por tanto, concluye el autor que la filosofía no debe ser tampoco la servidora de ninguna otra disciplina o actividad
Filosofía y silencio en Wittgenstein
No full textIn this paper is analysed the meaning of philosophy of Wittgenstein and the role played in it by the silence and the ethic and existential attitude proposed by this author. In this sense, it pays a special attention to the way in which Wittgenstein thought the social and cultural traits of his times, particularly the idea of progress
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