291 research outputs found
I CentoQuindici passi
No full textAnalisi del progetto "Villa CentoQuindici Passi" avente l'obiettivo di individuare buone pratiche in materia di riutilizzo sociale dei beni confiscati alle mafie
Indagine sulle relazioni tra inquinamento atmosferico e mortalità e ricoveri ospedalieri nella città di Parma
No full textIl cordone libero meccanizzato dalla potatura alla vendemmia
No full textIl vitigno Sangiovese ha portamento e crescita dei germogli di tipo intermedio tra assurgente (verso l’alto) e procombente (verso il basso) e tale caratteristica, associata alla notevole fertilità delle gemme basali, consente di adottare potature invernali molto corte e perfettamente integrate con la potatura meccanic
Il cordone libero meccanizzato dalla potatura alla vendemmia
No full textIl vitigno Sangiovese ha portamento e crescita dei germogli di tipo intermedio tra assurgente (verso l’alto) e procombente (verso il basso) e tale caratteristica, associata alla notevole fertilità delle gemme basali, consente di adottare potature invernali molto corte e perfettamente integrate con la potatura meccanic
Il cordone libero meccanizzato dalla potatura alla vendemmia
No full textIl vitigno Sangiovese ha portamento e crescita dei germogli di tipo intermedio tra assurgente (verso l’alto) e procombente (verso il basso) e tale caratteristica, associata alla notevole fertilità delle gemme basali, consente di adottare potature invernali molto corte e perfettamente integrate con la potatura meccanic
Recovery tests of cytopathogenic viruses from artificiallycontaminated food samples
No full textAbstract
The artificial contamination tests were carried out by using a Coxsackie B5 virus of known titration to contaminate vegetable food products (lettuce and berries). The experimental protocol was divided basically into two phases: elution with alkaline
pH buffer solution and following concentration of viral particles recovered by using PEG8000 (polyethylene glycol). A third phase of purification with chloroform was introduced between these two steps in order to assess its effect on the yield of the final recovery, and tests were performed in parallel with both the
protocols to compare them in terms of recovery efficiency. Elution phase proved to be the most critical, since the viral recovery from food samples during this phase resulted moderate (2.95% and 2.16% respectively in tests without and with chloroform purification phase), data already observed in previous studies. The
final concentration phase with PEG8000 recorded average recoveries equalling 0.29% in tests without chloroform and equalling 3.97% in tests with purification phase, thus showing a significant improvement with a lesser interference by the
organic material
PCR, Real-Time PCR analysis on Norwalk virus direct test on artificial-contaminated foodstuff
No full textIntroduction: The most commonly used methods to determine and identify Norwalk virus are based on molecular biology. Methods: A viral extraction protocol from food samples was studied in this work using artificial contamination test. It consists of a new protocol with a phase of viral elution from the food matrix performed using an eluting solution (glycine and beef extract at 3% pH 9) and a concentration phase with polyethylene glycol 8000. To detect Noroviruses, two techniques of molecular biology, polymerase chain reaction and real-time polymerase chain reaction, were compared. At the same time, tests of direct viral identification were conducted on soft fruits and salad obtained from the market. Results: From the results obtained it was possible to evaluate how the phase of viral recovery represents an important critical point of the protocol. Conclusion: It was possible to identify a greater sensitivity of the real-time polymerase chain reaction compared with the traditional polymerase chain reaction
High hydrostatic pressure activity on the disinfection of clams artificially contaminated with feline calicivirus
No full textThe contamination of shellfish with gastroenteric viruses may cause outbreaks because they are often eaten raw or under-cooked. High-hydrostatic pressure treatments have already proven to be effective in reducing high viral load in shellfish samples. The objectives are the assessment of the viral load reduction of contaminated clams using HHP treatments at different pressures and times and the study of the changes caused by these treatments in some food physical parameters. Clams were contaminated with a solution containing Feline Calicivirus; they were closed in envelopes and treated with 300, 400, 500, 600 MPa for 1, 3, 5, 7 min for every pressure value. After the treatment the residual viral titre was calculated. The texture parameters were obtained after treating clams samples at the same pressure values but only for 3 and 7 min and analysing them with a TPA test. HHP treatments of 500 and 600 MPa were sufficient to cause a total inactivation at every timelength considered while with 300 and 400 MPa after 1 min, concentrations of 1.13 and 0.55 respectively were found. In general hardness and gumminess tend to increase after the treatment whereas springiness and cohesiveness decrease a bit. HHP treatments showed good sterilization ability against FCV but it's necessary to consider that FCV has a lower resistance to disinfection than Human norovirus. Texture changes are in line with what is reported in literature
Photopolymerization of keratin-based thiol-ene coatings
No full textKeratin extracted from wool by sulphitolysis, up to a content of 15 wt/wt% was used as a natural reinforcing agent for cross-linked polymers obtained by photopolymerization of thiol-ene systems. The keratin-based photocured coatings obtained show significant improvement in terms of thermal stability, crosslinking density, Tg values and mechanical properties. Moreover, a clear increase of the biodegradability of the starting thiol-ene polymer matrix was induced by the presence of the keratin biopolymer as reinforcemen
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