1,721,364 research outputs found
La sostenibile leggerezza della pietra. Dall’architettura rupestre alla progettazione biofila
No full textThis contribution reflects on a broader field of investigation, developed through the activities of the Nature City LAB at the University of Basilicata, with particular reference to the research on biophilic design conducted by the author and the team she coordinates. Specifically, a trajectory is outlined below that explores the relationship between the urban ecosystem, architecture, and stone. Taking the Sassi of Matera as a privileged observation point, this exploration is oriented towards defining a perspective situated in a field of research-action defined by a double binomial: rock habitat/sustainability, biophilic design/regeneration
Proceedings - VRCAI 2013: 12th ACM SIGGRAPH International Conference on Virtual-Reality Continuum and Its Applications in Industry: Preface
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Lina Ghotmeh. The Archeologist of the Future
No full textThis is a journey to discover the work of Lina Ghotmeh – Architecture. It’s a survey on its innovative and poetic work. An essay, a selection of projects and an interview reveal an in-depth, historical, and materially sensitive approach to its projects. Lina Ghotmeh defines her work as ‘Archeology of the future’. The archeology of the future is a way to reinvent the traces of the past. Space is thought of as a palimpsest of stories, of narratives, but also of specific conditions that the architect brings to the surface. It is an approach in which the phenomenological dimension is founded with a rigorous knowledge of the natures – environmental, cultural, social – of the context. Accessing Lina Ghotmeh’s architectures means intercepting the hermeneutic relations between the different phases of their design process
A new two-step precipitation method removes free-SDS and Thiol reagents from diluted solutions, and then allows recovery and quantitation of proteins
No full textBiochem Biophys Res Commun. 1994 Apr 29;200(2):916-24.
A new two-step precipitation method removes free-SDS and thiol reagents from diluted solutions, and then allows recovery and quantitation of proteins.
Carraro U, Doria D, Rizzi C, Sandri M.
Source
Department of Experimental Biomedical Sciences, University of Padua, Italy.
Abstract
Several polypeptides are present in a few copies per cell (i.e., membrane receptors or transcription factors), and therefore their concentration and quantitation from highly diluted solution after detergent solubilization is often an essential and difficult step in their purification by gel electrophoresis. A solution (optimized to Tris-glycine, Tris-borate, and Na-phosphate buffers) to all of these problems is here described, detailing a two-step procedure which takes advantage of the lower solubility of free potassium dodecyl sulfate (KDS) vs micellar KDS even at neutral pH. In the first step, more than 90% of free DS precipitates out from protein solution, the method being poorly sensitive to dodecyl sulfate/KCl ratio from almost no SDS to 10%. Indeed to obtain a residual 0.01% of SDS in the solution no adjustment of KCl concentration is needed from 0.1 to 2.3% SDS. When the supernatant is added with ice-cold TCA and K+, protein solubility is severely affected. Excellent protein recovery, at least 90%, is obtained with hydrophilic proteins. On the other hand, hydrophobic or low-ionic-strength-insoluble proteins are partially lost in step 1 precipitate, so that protein yield decreases, still remaining to about 80%. Furthermore our procedure simplifies determination of protein contents of diluted mercaptoethanol-SDS-solubilized proteins: since thiol reagents are discarded with the final supernatant, proteins can be quantitated in the final pellet by standard colorimetric methods.
PMID:
8179627
[PubMed - indexed for MEDLINE
The fourth landscape: ecological restoration, mitigation and compensation as design strategies
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