86,643 research outputs found
Variabili strutturali e comportamenti socio-economici: metodi demografici per analisi comparative
No full textI metodi di standardizzazione permettono di neutralizzare l'effetto di fattori di possibile distorsione sul confronto, ad esempio, di mercati simili per dimensione ma di diversa composizione socio-demografica, per analizzare le diverse propensioni all'acquisto o al consumo di un bene o servizio
Applied Demography.La Demografia per le aziende e la governance locale
No full textIl volume illustra come, per gli operatori economici del mercato definito da un territorio e una popolazione di consumatori, utenti, lavoratori e aziende, molti ambiti di studio della Demografia abbiano una importante valenza per le attività di governance. Questa valenza passa per la conoscenza sia delle più importanti dinamiche di popoazione che dei metodi demografici adeguati ad effettuare previsioni, confronti, valutazioni di mercato
Marital breakdowns in Italy: recent regulatory changes and territorial analysis
No full textIn the last years, two laws have deeply changed the regulation of marital breakdowns in Italy. Between these two, the law n. 162/2014 introduces the possibility of employing extrajudicial agreements for consensual separations and divorces and paves the way for more innovative behaviors, among couples without minor or economically dependent children. By the means of spatial statistical tools, the paper detects the geographical (at province/NUTS-3 level) pattern of the incidence of extrajudicial agreements for marital breakdowns over total number of separations and divorces. The spreading of these new procedures resulted as not homogeneous on the Italian territory as the individuals living in neighbouring provinces tend to display similar relative levels of extrajudicial agreement
Modelling Network Data: an Introduction to Exponential Random Graph Models
No full textA brief introduction to statistical models for complete network data is presented. An example is provided by the collaboration network of Italian scholars on Population Studies. Results show some evidence that higher order model specification is more effective than the Markov random graph model, overcoming the convergence problem of classical Markov formulation
Population ageing and human resources management. A chance for Applied Demography
No full textThe population ageing has become a global and worldwide phenomenon. Especially in Italy this process is strong and incontestable. In this scenario the work force ageing and the challenges that make urgent to find tools and measures to manage the oldest people can give to the “applied demographer” a significant role. Moving from the definition of active ageing we illustrate skills, activities and suggestions that a demographer could easily share with human resource managers interested in promoting strategies and policies enhancing employability and productivity of older workers. We report real experiences with ABB Italia, SOL Group and Italcementi. We finally give details on AMO (Age Management Observatory)
A consensus motif common to all Rho-dependent prokaryotic transcription terminators
No full textWe have characterized at the molecular level several polar mutations in four different cistrons of the his operon of S. typhimurium. An analysis of the his-specific transcripts produced in vivo in the mutant strains, together with in vitro transcription assays, led to the identification of several cryptic Rho-dependent transcription termination elements within the his operon that are activated by the uncoupling of transcription and translation. Common features of these elements were sought and found with a computer program. We have identified a consensus motif, consisting of a cytosine-rich and guanosine-poor region, that is located upstream of the heterogeneous 3' endpoints of the prematurely terminated in vivo transcripts and that is present in all the Rho-dependent transcription terminators described thus far
Alternative patterns of his operon transcription and mRNA processing generated by metabolic perturbation
No full textPrevious studies have shown that the expression of the his operon of Salmonella typhimurium is regulated at the level of transcription initiation, transcription elongation and RNA processing. We have analyzed his RNA in both prototrophic strains or strains harboring regulatory and auxotrophic mutations grown under a variety of metabolic conditions that lead to differential expression of the operon. Under some of these conditions, there is an increase in the amount of prematurely released his-specific RNA, resulting in modulation of the relative amount of full-length transcripts. Under the same metabolic conditions, there is also a modulation of RNA processing events that generate a very stable RNA species comprising the five distal cistrons. These effects appear to be due to perturbation of the translation process caused by alterations in the intracellular pool of initiator transfer RNA
Are Real World Data the smart way of doing Health Analytics?
No full textReal world data (RWD) and real world evidence (RWE) are playing an increasing role in health care. Despite their use may strongly improve healthcare research as well as health related decision making, management and planning, many barriers remain to their use in clinical practice. The aim of this paper is to discuss some issues related to RWD aiming at proposing a new paradigm of healthcare research based on RW
Ribonuclease E provides substrates for ribonuclease P-dependent processing of a polycistronic mRNA
No full textThe polycistronic mRNA of the histidine operon is subject to a processing event that generates a rather stable transcript encompassing the five distal cistrons. The molecular mechanisms by which such a transcript is produced were investigated in Escherichia coli strains carrying mutations in several genes for exo- and endonucleases. The experimental approach made use of S1 nuclease protection assays on in vivo synthesized transcripts, site-directed mutagenesis and construction of chimeric plasmids, dissection of the processing reaction by RNA mobility retardation experiments, and in vitro RNA degradation assays with cellular extracts. We have found that processing requires (1) a functional endonuclease E; (2) target site(s) for this activity in the RNA region upstream of the 5' end of the processed transcript that can be substituted by another well-characterized rne-dependent cleavage site; (3) efficient translation initiation of the first cistron immediately downstream of the 5' end; and (4) a functional endonuclease P that seems to act on the processing products generated by ribonuclease E. This is the first evidence that ribonuclease P, an essential ribozyme required for the biosynthesis of tRNA, may also be involved in the segmental stabilization of a mRNA
A tRNA gene mapping within the chloroplast rDNA "cluster" is differentially expressed during the development of Daucus carota.
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