68 research outputs found
Fuzz-Web: A Methodology Based on Fuzzy Logic for Assessing Web Sites
No full textAbstract: This paper presents a quality assessment methodology and model that measure the performance of dynamic websites. Called Fuzz-Web, a system that shows a comprehensive and natural manner of reasoning based on Multiple Criteria Decision Making process. We attempt so to use fuzzy logic as an intelligent technology, since the evaluation process is characterized by subjectivity and imprecision. Obviously, a phase of selecting appropriate evaluation criteria is necessary for the decision making process. Some tests realized on a set of Tunisian and foreign websites allow us to discuss the proposed reduced fuzzy method and then to validate the decision making result
Quality evaluation of web sites: A comparative study of some Multiple Criteria Decision Making methods
No full text
Extraction of association rules used for assessing web sites' quality from a set of criteria
No full textAssessing web sites quality: A systematic literature review by text and association rules mining
No full textDevelopment of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, <it>Yarrowia lipolytica</it>
No full textAbstract Background As an oleaginous yeast, Yarrowia lipolytica is able to assimilate hydrophobic substrates. This led to the isolation of several promoters of key enzymes of this catabolic pathway. Less is known about the behavior of Y. lipolytica in large bioreactors using these substrates. There is therefore a lack of established know-how concerning high cell density culture protocols of this yeast. Consequently, the establishment of suitable induction conditions is required, to maximize recombinant protein production under the control of these promoters. Results Human interferon α2b (huIFN α2b) production in Yarrowia lipolytica was used as a model for the enhancement of recombinant protein production under the control of the oleic acid (OA)-inducible promoter POX2. Cell viability and heterologous protein production were enhanced by exponential glucose feeding, to generate biomass before OA induction. The optimal biomass level before induction was determined (73 g L-1), and glucose was added with oleic acid during the induction phase. Several oleic acid feeding strategies were assessed. Continuous feeding with OA at a ratio of 0.02 g OA per g dry cell weight increased huIFNα2b production by a factor of 1.88 (425 mg L-1) and decreased the induction time (by a factor of 2.6, 21 h). huIFN α2b degradation by an aspartic protease secreted by Y. lipolytica was prevented by adding pepstatin (10 μM), leading to produce a 19-fold more active huIFN α2b (26.2 × 107 IU mg-1). Conclusion Y. lipolytica, a generally regarded as safe (GRAS) microorganism is one of the most promising non conventional yeasts for the production of biologically active therapeutic proteins under the control of hydrophobic substrate-inducible promoter.</p
Protective effect of Cinnamomum zeylanicum L. bark essential oil, on hepatic and renal toxicity induced by CCl4 in rats
No full textThe inner bark of cinnamon (Cinnamomum zeylanicum L.) is widely used as a spice. Cinnamomum plants are also a valuable sources of essential oil used for medicinal purposes. The present study aimed to investigate: the composition, the in vitro antioxidant activity of C. zeylanicum bark essential oil (CzEO) and its protective effects in vivo on CCl4-induced hepatic and renal toxicity in rats.
Groups of animals were pretreated for seven days with different concentrations of CzEO or controls and on day 7 a single dose of CCl4 was used to induce oxidative stress in rats. Twenty-four hours after CCl4 administration, the animals were sampled. In the controls, CCl4 induced an increase of serum biochemical parameters and triggered oxidative stress in both liver and kidneys. CzEO (100 mg/kg) caused significant reductions in CCl4-elevated levels of ALT, AST, ALP, LDH, ÎłGT,total cholesterol, triglycerides, LDL urea and creatinine and increased the level of HDL compared to the CCl4 group. Moreover, pre-treatment with the CzEO at doses of 70 and 100 mg/kg BW to the rats treated with CCl4 produced significant reductions in TBARS and PCO levels in liver and kidney tissues as compared to CCl4 group. The formation of pathological hepatic and kidney lesions induced by the administration of CCl4 was strongly prevented by CzEO at a dose of 100 mg/kg BW.
Overall, this study suggests that administration of CzEO displayed high potential to quench free radicals and alleviate CCl4-induced hepatorenal toxicity in rats.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Cyclic Pressuremeter Tests Dedicated to Study the Behavior of Piles Under Cyclic Transverse Loads
No full textInternational audienceWe propose a cyclic pressuremeter (CPM) test protocol deduced from the reaction of the soil-pile values derived from tests of cyclic transverse loads on pile. The relative displacements issued from CPM tests are compared to the relative displacements of the pile under cyclic transverse loads
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