306,978 research outputs found

    Gravidanza e contesti psicopatologici. Dalla teoria agli strumenti di intervento

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    Un testo di pratica clinica, rivolto a medici, ostetriche, operatori socio-sanitari, psicologi. Illustra le diverse situazioni problematiche della gravidanza e del puerperio (PTSD, ansia, depressione, sessualità, diagnosi prenatale, parto, IVG, morte endouterina, vulnerabilità psicopatologica, prematurità) e i modelli, protocolli e suggerimenti specifici per ciascun contesto

    ISOELECTRIC-FOCUSING IN NON-AMPHOTERIC BUFFERS - CATASTROPHE AND NON-CATASTROPHE THEORIES

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    Four potential modes of isoelectric focusing in non-amphoteric buffers are evaluated:(a)"stack" or "train" of free bases or acids "arrested by a deprotonation or protonation mechanism", respectively (Chrambach); (b) "chemically bonded" (immobilized) pH gradients (Righetti et al.); (c) "physically bonded" or "quasi-immobilized" pH gradients (Bier et al.); (d) steady-state rheoelectrolysis (Rilbe). The first is based on a "catastrophe" theory, i.e., it confines the buffers in a pH region where they can create a pH gradient by an isotachophoretic mechanism, but where they do not have sufficient buffering capacity to stabilize it; no true isoelectric focusing can ever be achieved with this system. The last three are based on sound and well defined theories; however, at present, only system (b) (immobilized pH gradients) has proved to be a simple and reliable technique, easily transplantable in any laboratory. Bier et al.'s and Rilbe's approaches require complex and elaborate experimental set-ups and strict adherence of laboratory practice to a set of physical laws governing the system. In practice, owing to the divergence of experimental approaches from idealized physical equilibria, the last two approaches appear still to be far away from daily laboratory work

    2-DIMENSIONAL MAPS IN VERY ACIDIC IMMOBILIZED PH GRADIENTS

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    Up to the present time it has been impossible to perform two-dimensional (2-D) separations in very acidic immobilized pH gradients (IPG), due to the lack of suitable buffering acrylamido derivatives to be incorporated into the polyacrylamide matrix. The advent of the pK 3.1 buffer (2-acrylamido glycolic acid; Righetti et al., J. Biochem. Biophys. Methods 16, 1988, 185-192) allowed the formulation of such acidic gradients. We report here separations in IPG pH 2.8-5.0 intervals of polypeptide chains from total lysates of rat intestinal and liver cells and 30S and 50S ribosomal proteins from Halobacterium marismortui. Conditions are given for highly reproducible first and second dimensions gels and for a proper silver staining of 2-D maps with practically no background deposition

    Sfide e limiti del veganismo. Vegefobia, mass media e mercato

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    Righetti, N. (2019). Sfide e limiti del veganismo. Vegefobia, mass media e mercato. In Bertuzzi, N., Reggio, M. (eds). «Smontare la gabbia. Anticapitalismo e movimento di liberazione animale», Mimesis, pp. 17-26

    Canchim: passado, presente e futuro.

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    A partir da década de 1940, diversas raças foram desenvolvidas com o uso do cruzamento de raças zebuínas, comprovadamente adaptadas ao clima tropical, com raças europeias de maior produtividade. Nos EUA, por exemplo, foi desenvolvida a raça Santa Gertrudis (5/8 Shorthorn e 3/8 Zebu), por meio de cruzamento alternativo e posterior seleção massal. Em gado leiteiro, temos o exemplo das raças Jamaica Hope e, no Brasil, temos a raça Girolando. A raça Canchim (5/8 Charolês e 3/8 Zebu) foi desenvolvida na Fazenda de Criação de São Carlos, SP (atualmente Embrapa Pecuária Sudeste), nessa mesma época (MOTTA, 1977).Edição técnica: Cintia Righetti Marcondes; Milena Ambrosio Telles

    L'esperienza valida. Teorie e pratiche per riconoscere e valutare le competenze

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    rassegna di orientamenti metodologie e disposititvi per il riconoscimento e la certificazione delle competenze in ambito non formale e informal
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