1,430,242 research outputs found
Estuary-ria exchange of cadmium, lead and zinc in the coastal system of the Ria of Vigo (NW Iberian Peninsula)
No full textPrego, R., Cobelo-García, A., Santos-Echeandía, J., De Castro, M., Ospina-Alvarez, N., & García-Pérez, M. (2010). Estuary-ria exchange of cadmium, lead and zinc in the coastal system of the Ria of Vigo (NW Iberian Peninsula). Scientia Marina, 74(S1), 77–87. doi:10.3989/scimar.2010.74s107
Exchange of nutrients across the sediment-water interface in intertidal ria systems (SW Europe)
No full textOspina-Alvarez, N., Caetano, M., Vale, C., Santos-Echeandía, J., Bernárdez, P., & Prego, R. (2014). Exchange of nutrients across the sediment-water interface in intertidal ria systems (SW Europe). Journal of Sea Research, 85, 349–358. doi:10.1016/j.seares.2013.07.00
RIA PROJECTS: reactivation, inclusion and reintegration projects.
No full textreservedNel mio lavoro di tesi ho deciso di affrontare l'argomento dei progetti Ria, ossia Reddito di Inclusione Attiva. Ho deciso di trattare questo argomento in quanto durante il mio secondo modulo di tirocinio ho fatto esperienza nell'area della marginalità adulta e ho avuto modo di assistere ai percorsi di inclusione di alcune persone che hanno partecipato a questo tipo di progetti. Nel primo capitolo ho deciso di inserire un quadro dei problemi emergenti e del diffondersi della povertà nel periodo post-covid, per poi concentrarmi nel secondo capitolo sui progetti RIA come misura di contrasto alla povertà, ed infine nel terzo capitolo proporre una parte più esperienziale con interviste ai protagonisti dei progetti Ria, evidenziandone le opportunità di reinserimento e riattivazione.In my thesis work, I decided to address the topic of Ria projects, or Active Inclusion Income. I decided to deal with this topic because during my second internship module I gained experience in the area of adult marginality and I had the opportunity to witness the inclusion paths of some people who participated in these types of projects. In the first chapter I decided to include an overview of the emerging problems and the spread of poverty in the post-covid period, and then to focus in the second chapter on RIA projects as a measure to combat poverty, and finally in the third chapter I decided to propose a more experiential part with interviews with the protagonists of RIA projects, highlighting the opportunities for reintegration and reactivation
Ecologia de Atherina spp. ao longo do gradiente salino do canal de Mira (Ria de Aveiro)
No full textMestrado em Ecologia, Biodiversidade e Gestão de EcossistemasAs espécies Atherina boyeri Risso, 1810 e Atherina presbyter Cuvier,
1829, apesar de apresentarem características merísticas e morfométricas
distintas, são por vezes difíceis de distinguir (nomeadamente em casos onde
os peixes são pequenos ou estão danificados). Estas duas espécies
apresentam também comportamento e ecologia distintos: A. presbyter é
tipicamente marinha, podendo estar presente nas áreas mais salinas dos
estuários, A. boyeri pode completar todo o seu ciclo de vida em água doce ou
salobra sendo o seu comportamento nos estuários desconhecido. Pouco se
sabe acerca da biologia destes peixes em Portugal, incluindo informação sobre
as populações estuarinas e conservação.
A amostragem foi efectuada no Canal de Mira (um dos braços
principais da Ria de Aveiro, Portugal) em intervalos de 3 meses (Verão 2007-
Primavera 2008) e foram determinados alguns parâmetros biométricos como
comprimento total, GSI e alguns factores de condição. A Ria de Aveiro foi
escolhida como caso de estudo de modo a determinar a influência do habitat,
nomeadamente do gradiente de salinidade na distribuição e ecologia da
população de peixe-rei. A distinção entre A. presbyter e A. boyeri foi feita com
base em caracteres morfológicos e moleculares. O presente trabalho permitiu
concluir que A. presbyter está presente em locais mais perto da boca do
estuário enquanto A. boyeri tem preferência pelos locais no final do canal. As
espécies coexistem em zonas intermédias (sainidade 5,5 a 22), com clara
dominância de A. presbyter sobre A. boyeri. A presente população de peixe-rei
reproduz-se durante a Primavera e Verão e os juvenis apenas se reproduzem
na época reprodutora seguinte após o seu nascimento. Foi também possível
concluir que há uma grande discrepância relativamente aos critérios merísticos
sugeridos pela bibliografia usados para distinguir as duas espécies, pelo que é
aqui proposto uma complementaridade entre critérios morfológicos e
genéticos.
ABSTRACT: Atherina boyeri Risso, 1810 and Atherina presbyter Cuvier, 1829,
although distinct in meristic and morphometric characters, are sometimes
difficult to differentiate, especially when small or injured fish are involved. They
also exhibit distinct ecology and behaviour: while A. presbyter is typically
marine, entering the more saline sections of estuaries, A. boyeri may complete
its life cycle in fresh or brackish waters, although its behaviour in estuaries is
unknown. Little is known on the biology of both species in Portugal, including
the estuarine populations, and basic information needed for their conservation
is missing.
A sampling campaign was carried out in Canal de Mira (one of the
main arms of Ria de Aveiro, Portugal) every three months (summer 2007 –
spring 2008), and biometric features such as total length, GSI, and condition
factors were determined. To avoid misidentifications, specimens were
diagnosed with the aid of morphological and molecular characters. Ria de
Aveiro was chosen as a case study to determine the influence of habitat,
namely the salinity gradient, in the distribution and ecology of the sand smelts
assemblage. We observed that A. presbyter occurred mostly downstream,
while A. boyeri preferred the upper reaches of the estuary and both species cooccurred
in the intermediate zones (salinity 5.5 to 22), with A. presbyter being
dominant in this zone. Reproduction of Canal de Mira assemblage occurred
during spring and summer, and juveniles only reproduced in the next
reproductive season after their birth. We also concluded that there is a large
overlap in scale counts between the two species, so a complementarity
between morphological and genetic criteria is here proposed
Episode Two: Ria Righteous
No full textIn this first full conversation, Rajni Shah and Ria Righteous take time to allow words to emerge between them through deep listening. The conversation is intimate and personal, meditating on themes of grief, selfhood, family, renewal, and the long and difficult work of dearmouring the heart and body.
This episode is accompanied by a short offering in which Ria guides the listener through a meditation called ‘Breathing the Body’. The offering was recorded by Ria in Kersal Dale, near the river Irwell in Salford.
Click here for full episode and credits
Narrativas Imagéticas
No full textA sociedade contemporânea convive com novas tecnologias, renovados ambientes comunicacionais, diferentes atores midiáticos e, por consequência, novas linguagens, onde a imagem ganha crescente protagonismo. O olhar a essa nova sociedade é intenso a partir da ecologia dos meios, corrente teórico-filosófica preocupada em observar, experimentar e compreender a relação entre a sociedade e os meios, em suas diversas conformações.
Percebe-se, a partir de diversos estudos desenvolvidos por ecologistas dos meios, que os processos comunicacionais têm sido realizados cada vez mais com o apoio nas imagens. Essa adoção crescente de narrativas imagéticas na comunicação pode ser justificada pelo desenvolvimento de dispositivos portáteis e de alta qualidade no registro e na difusão. E parece mesmo ser uma realidade contemporânea.
Diante disso, oferecemos a você, interessado em compreender o cenário do ecossistema midiático contemporâneo, tendo como reconhecimento o protagonismo da imagem, essa obra, aberta a download e compartilhamento totalmente grátis. Essa é a filosofia do MEISTUDIES, da Universidade Técnica Particular de Loja – UTPL e da Ria Editorial, que dedicam-se a construir conhecimento científico de forma democrática, aberta e acessível. Boa leitura.http://www.riaeditorial.com/index.php/narrativas-imageticas
Qual a importância das trocas entre a Ria Formosa e o oceano adjacente para a produtividade biológica destes sistemas?
No full textA Ria Formosa é a lagoa costeira mais importante do sul de Portugal, com grande influência nas trocas de água e de matéria com o oceano adjacente, as quais controlam a produtividade biológica destes ecossistemas intimamente ligados. Neste capítulo são apresentadas as características geomorfológicas da Ria Formosa e as características dinâmicas que a tornam um sistema bastante produtivo e peculiar, com forte impacto no oceano adjacente. São ainda apresentadas, pela primeira vez, as trocas através das três barras do setor oeste da Ria Formosa, em condições de maior produtividade biológica, típicas de Primavera, evidenciando a importância da interligação entre estes dois sistemas, particularmente num período sob afloramento costeiro.info:eu-repo/semantics/publishedVersio
Network analysis to dissect glioblastoma immunosuppression complexity: identification of C-type lectins as potential therapeutic candidates and biomarkers
No full textIntroduction: Glioblastoma (GBM) is one of the most threatening tumors in adults. The failure of treatments, GBM aggressiveness and the poorness of patients’ quality of life underline the unmet need to identify novel therapeutic targets. The strong immunosuppression, hypoxia and altered vasculature make account for GBM resistance. Lectins are immunoregulatory receptors, that in tumors promote immunosuppression. Lectins expression on distinct cell type (i.e.myeloid, lymphoid and cancer cells) and the distribution of their respective ligands generate a complex and intricate network of interactions that support immunosuppressive mechanisms. So far, little is known about lectins contribution to immunosuppression and progression in GBM.
Aims: to investigate the lectin profile relevant in glioblastoma immunosuppression exploiting network-based approaches and to investigate their possible role as immune biomarkers and target
Results: Using three public databases (TCGA and CGGA for GBM; GTEx for healthy brain tissue), we selected a panel of 67 lectins from the Siglec, galectin, and C-type lectin families. Setting rigorous filtering parameters based on lectins expression levels, a final set of 39 lectins was employed to construct co-expression and differential co-expression networks. The analysis revealed significant correlations between lectin profiles across TCGA and CGGA. C-type lectins, that have never been investigated in GBM, were the most represented (ASGR2, CLEC12A, CLEC10A, CLEC4A, CLEC7A, CLEC2B, CD209) followed by Siglec7, Siglec9 and galectin-9, that have been already described in GBM progression and immunosuppression. The results obtained by computational analysis were validated on patients-matched tumor samples and liquid biopsies. CLEC10A was expressed in GBM-infiltrating immune cells and particularly its high expression on infiltrating macrophages correlated with poor survival. Distinct CLEC10A+ myeloid cells subsets correlate with different T cell infiltrate. Indeed, CLEC10A+ macrophages were positively associated with activated CD4+ T cells, that are likely to be regulatory T cells, while CLEC10A+ LOX1+ PMN-MDSCs negatively correlate with effector and antitumor CD8+ T cells. These results underline that distinct CLEC10A+ myeloid cells may be involved in different immunomodulatory mechanisms in GBM.
CLEC10A ligands Tn and STn were expressed in GBM tissues. Tn is exclusively expressed on cancer cells in GBM in correspondence of macrophages, while STn was expressed also in low-grade gliomas in vascular area. These results highlight a possible role of CLEC10A-Tn axis in GBM TME and immunosuppression, as already described in other cancer histytopes. However, GBM immunosuppression is complex and likely several actors may contribute, thus the intricate network of interactions is not associated to a single lectin and a single cell type. The novel C-type lectins cluster (ASGR2, CLEC12A, CLEC4A, CLEC7A) identified by network analysis was investigated in GBM patients. ASGR2 and CLEC12A emerged as strongest hub of both GBM differential co-
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expression networks and low levels of both lectins were associated with improved prognosis. Combining ASGR2 and CLEC12A expression with lectins of other families already known (siglec7, siglec9, and galectin9), we identified a lectin profile that better discriminated survival of GBM patients. So far, no evidence of these C-type lectins is available in cancer. We found that all C-type lectins are expressed on GBM-infiltrating immune cells. ASGR2 levels were significantly higher on MDMs compared to resident microglia (MG) and it was undetectable on circulating immune cells, suggesting a specific role in the GBM TME. CLEC7A expression was higher in macrophagic subpopulations compared to MDSCs. CLEC4A and CLEC12A were highly expressed on all circulating immune cell subsets, particularly those of monocytic origin. Their expression was significantly higher on circulating cells than in infiltrating cells. Similar lectin expression patterns were observed in patients with other cancers (NSCLC, head and neck cancer, HER2+ breast cancer), indicating that CLEC4A and CLEC12A may be relevant across different tumor types.
Conclusions:
We described for the first time C-type lectins cluster (ASGR2, CLEC4A, CLEC7A, CLEC12A, CLEC10A) that have never been described in GBM by exploiting network-based approaches. CLEC10A-Tn axis emerged as possible mechanism that supports GBM immunosuppression, underlining an interplay between macrophages and cancer cells. Moreover, ASGR2 expression appeared to be restricted to GBM TME and in association to macrophages and could represent a lectin that is induced under pathological context and may be involved in immunosuppressive mechanisms. The C-type lectins profile appears to be associated to distinct immunosuppressive myeloid cell subsets that are found not only in GBM TME but also peripheral blood, underlining their possible role as circulating immune biomarkers. Further analysis is required to investigate the metabolic and transcriptomic profile of lectins-expressing myeloid cells. Moreover, identifying the lectins ligands and understanding the impact of their engagement on the myeloid cells may be relevant to identify novel possible target to be exploited in GBM treatment. Investigating changes in lectin expression post-surgery or treatment, along with their relationship to immune features and clinical outcomes, could provide valuable insights into their roles in tumor progression and response to therapies
Radioimmunoassay (RIA) módszer kifejlesztése szomatosztatin peptidhormon kvantitatív meghatározására
No full textRadioimmunoassay módszert fejlesztettünk ki a szomatoszatin elnevezésű peptidhormon kvantitatív meghatározására. Célunk az volt, hogy szöveti és plazma peptid koncentrációk meghatározásával vizsgálni tudjuk a peptid biológiai szerepét. A RIA módszer kifejlesztése során a C-terminális érzékenységű ellenanyagot nyulak immunizálásával termeltettük marha szérum albumin–szomatoszatin-14 (BSA-SOM-14) immunogén szubkután adásával. Az alkalmazott antiszérum egyforma mértékben méri a peptid két biológiailag aktív formáját a SOM-14-et és a SOM-28-at. A kifejlesztett szomatosztatin RIA módszerünk nagy érzékenységű és peptid specifikus. Alkalmas arra, hogy szöveti és plazma koncentráció meghatározásokat végezzünk vele.VegyészMSc/M
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