1,497 research outputs found
Thermodynamic assessment of the KF-ThF<sub>4</sub>, LiF-KF-ThF<sub>4</sub> and NaF-KF-ThF<sub>4</sub> systems
No full textA thermodynamic assessment of the KF-ThF4 binary system using the CALPHAD method is presented, where the liquid solution is described by the modified quasichemical formalism in the quadruplet approximation. The optimization of the phase diagram is based on experimental data reported in the literature and newly measured X-ray diffraction and differential scanning calorimetry data, which have allowed to solve discrepancies between past assessments. The low temperature heat capacity of α-K2ThF6 has also been measured using thermal relaxation calorimetry; from these data the heat capacity and standard entropy values have been derived at 298.15 K: Cp,mo(K2ThF6,cr,298.15K)=(193.2±3.9) J·K-1·mol-1 and Smo(K2ThF6,cr,298.15K)=(256.9±4.8) J·K-1·mol-1. Taking existing assessments of the relevant binaries, the new optimization is extrapolated to the ternary systems LiF-KF-ThF4 and NaF-KF-ThF4 using an asymmetric Kohler/Toop formalism. The standard enthalpy of formation and standard entropy of KNaThF6 are re-calculated from published e.m.f data, and included in the assessment of the ternary system. A calculated projection of the NaF-KF-ThF4 system at 300 K and the optimized liquidus projections of both systems are compared to published phase equilibrium data at room temperature and along the LiF-LiThF5 and NaF-KThF5 pseudobinaries, with good agreement.RST/Reactor Physics and Nuclear Material
Revised diagnostic criteria for Vogt-Koyanagi-Harada disease: report of an international committee on nomenclature
No full textKF-NIPT: K-mer and fetal fraction-based estimation of chromosomal anomaly from NIPT data
No full textBACKGROUND: Non-Invasive Prenatal Testing (NIPT) is a technique that allows pregnant women to screen for chromosomal abnormalities in their developing fetus without the need for invasive procedures like amniocentesis or chorionic villus sampling. However, current methods to detect anomaly from maternal cell-free DNAs (cfDNAs) that are based on the sequence read counts calculating z-scores face challenges with false positives and negatives. To address these challenges, we aimed to develop a novel NIPT algorithm named KF-NIPT, which is derived from the initials of k-mer and fetal fraction used in its development with the goal of significantly improving accuracy. RESULTS: We developed a KF-NIPT, a new algorithm that estimate chromosomal anomaly by calculating K-mer-based sequence depth and fetal fraction from the whole genome sequencing (WGS) data. Moreover, we implemented a modified preprocessing pipeline for the WGS data, correcting the biases of the genomic mapping quality and the GC contents. The performance of our method was evaluated using publicly available NIPT data. We could demonstrate that our method has better accuracy and sensitivity compared to those of the previous methods. CONCLUSIONS: We found that using k-mer and fetal fraction reduces errors in NIPT and have integrated this into a pipeline, showing that the traditional read count-based z-score method can be improved. KF-NIPT is implemented in the R and Python environment. The source code is available at https://github.com/eastbrain/KF-NIPT . KF-NIPT has been tested on Ubuntu Linux-64 server and Linux-64 on Windows using a WSL (Windows Subsystem for Linux)
A comprehensive evaluation of alignment algorithms in the context of RNA-seq.
No full textTranscriptome sequencing (RNA-Seq) overcomes limitations of previously used RNA quantification methods and provides one experimental framework for both high-throughput characterization and quantification of transcripts at the nucleotide level. The first step and a major challenge in the analysis of such experiments is the mapping of sequencing reads to a transcriptomic origin including the identification of splicing events. In recent years, a large number of such mapping algorithms have been developed, all of which have in common that they require algorithms for aligning a vast number of reads to genomic or transcriptomic sequences. Although the FM-index based aligner Bowtie has become a de facto standard within mapping pipelines, a much larger number of possible alignment algorithms have been developed also including other variants of FM-index based aligners. Accordingly, developers and users of RNA-seq mapping pipelines have the choice among a large number of available alignment algorithms. To provide guidance in the choice of alignment algorithms for these purposes, we evaluated the performance of 14 widely used alignment programs from three different algorithmic classes: algorithms using either hashing of the reference transcriptome, hashing of reads, or a compressed FM-index representation of the genome. Here, special emphasis was placed on both precision and recall and the performance for different read lengths and numbers of mismatches and indels in a read. Our results clearly showed the significant reduction in memory footprint and runtime provided by FM-index based aligners at a precision and recall comparable to the best hash table based aligners. Furthermore, the recently developed Bowtie 2 alignment algorithm shows a remarkable tolerance to both sequencing errors and indels, thus, essentially making hash-based aligners obsolete
Húsz év után. A Könyvtári Figyelő 2000–2019 között
No full textThe study provides an overview of the life of this peer-reviewed national professional journal edited at the Hungarian Library Institute in the period 2000 through 2019. Könyvtári Figyelő entered its 65th volume this year. This quarterly magazine has published 80 issues and more than 15,000 pages in PDF format in twenty years. Thanks to the support of the National Cultural Fund (NKA) printed issues continue to be published to our days. The full text of issues can be accessed and searched on an electronic interface (http://ki.oszk.hu/kf) from the beginnings (1955) to the present day (2020/1). Roughly two-thirds of each issue include research articles on the main events and developments in Hungarian librarianship, and one-third deals with the presentation of developments in the international library world. The author reviews the most important articles in the Studies, Our past, and Book reviews columns in two ten-year cycles, and refers to the professional trends summarized at the beginning of her article. The editors are increasingly oriented towards open access and the application of the Open Journal System (OJS).A tanulmány áttekintés a Könyvtári Intézetben készülő, idén 65. évfolyamába lépett, országos hatókörű, lektorált szakmai folyóirat 2000 és 2019 közötti működéséről. A negyedéves megjelenésű folyóirat húsz év alatt 80 füzetszámban, több mint 15 ezer pdf oldal terjedelemben jelent meg. Az NKA támogatásának köszönhetően ez idáig nyomtatott formában is elkészülhetett. Elektronikus felületén (http://ki.oszk.hu/kf) a kezdetektől (1955 től) napjainkig (2020/1.) elérhető és kereshető a lapszámok teljes szövege. Minden szám nagyjából kétharmadában részben a magyarországi könyvtárügy legfőbb eseményeiről és fejlesztéseiről közöl elméleti publikációkat, egyharmadában pedig a külföldi könyvtári világ fejleményeinek bemutatásával foglalkozik. Kétszer tíz éves ciklusra bontva tekinti át a szerző a Tanulmányok, a Múltunk és a Könyvszemle rovatok legfontosabb írásait, és utal a cikkek beszámolója elején felvillantott szakmai trendekkel való kapcsolatára. A szerkesztőség számára a jövő útja a nyílt hozzáférés és az Open Journal System (OJS) nyílt forráskódú szerkesztőségi rendszer kialakítása felé vezet
Thermodynamic assessment of the LiF-NiF<sub>2</sub>, NaF-NiF<sub>2</sub> and KF-NiF<sub>2</sub> systems
No full textUsing the modified quasi-chemical model in the quadruplet approximation, three new thermodynamic assessments of binary systems useful for the detailed operational design of the Molten Salt Reactor are presented: AF-NiF2 (A = Li, Na, K). These systems are particularly relevant for the study of the molten salt-structural materials interaction, as the salt containment is made of a Ni-based alloy. Using powder X-ray Diffraction (XRD) and Differential Scanning Calorimetry (DSC), new experimental data were gathered for two of these systems, LiF-NiF2 and KF-NiF2, and compared to previous experimental assessments. Our data have confirmed the formation of a (Li1-2xNix)F solid solution. The three thermodynamic models show a very good agreement with the experimental data. The melting point of NiF2 was measured for the first time to be T = (1629 ± 5) K, and the thermal expansion coefficient for Li2NiF4 was found to be α=27.6·10-6K-1 in the temperature range T = (298–773) K.Accepted Author ManuscriptRST/Reactor Physics and Nuclear Material
NLINLS: a Differential Evolution based nonlinear least squares Fortran 77 program
No full textThis paper provides the list of Fortran 77 codes of nonlinear least squares using Differential Evolution as the minimizer algorithm. It has been tested on a number of difficult nonlinear least squares problems (taken from NIST, USA including CPC-X Software challenge problems). Help on how to use the program also is provided.Nonlinear least squares; Differential Evolution; Fortran 77
Correction by contrastive focus
No full text'Correction' is the name of a sentence with contrastive focus' the phonological/phonetic realization of which is a single contrastive pitch accent. These sentences predominantly appear in (fictional) dialogues. The first speaker uses grammatical entities against which the next speaker protests with a sentence nearly identical except that it contains a prosodically marked corrective element. This paper makes contrastive focus visible by means of 'KF' (contrastive focus)
Micro-arc oxidization of a novel Mg-1Ca alloy in three alkaline KF electrolytes: Corrosion resistance and cytotoxicity
No full textA newly-developed Mg-1Ca (wt%) alloy was treated by micro-arc oxidization (MAO) in KF-silicate- (Si coating), KF-phosphate- (P coating) and KF-silicate-phosphate (SiP coating) electrolytes. The microstructure, composition and corrosion resistance of the resultant MAO coatings were investigated using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffractometry (XRD). Electrochemical analysis and immersion test in Hanks' solution and MU assay for in-vitro toxicity against MG63 cells were subsequently carried out. Results showed that all the three MAO coatings contributed to the improvement of corrosion resistance and cytocompatibility of substrate; however, P coating outperformed the two others due to its specific microstructure and composition. (C) 2013 Elsevier B.V. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000330208500143&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Chemistry, PhysicalMaterials Science, Coatings & FilmsPhysics, AppliedPhysics, Condensed MatterSCI(E)[email protected]
Real-time resolution of short-read assembly graph using ONT long reads
No full textA streaming assembly pipeline utilising real-time Oxford Nanopore Technology (ONT) sequencing data is important for saving sequencing resources and reducing time-to-result. A previous approach implemented in npScarf provided an efficient streaming algorithm for hybrid assembly but was relatively prone to mis-assemblies compared to other graph-based methods. Here we present npGraph, a streaming hybrid assembly tool using the assembly graph instead of the separated pre-assembly contigs. It is able to produce more complete genome assembly by resolving the path finding problem on the assembly graph using long reads as the traversing guide. Application to synthetic and real data from bacterial isolate genomes show improved accuracy while still maintaining a low computational cost. npGraph also provides a graphical user interface (GUI) which provides a real-time visualisation of the progress of assembly. The tool and source code is available at https://github.com/hsnguyen/assembly
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