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    Molecular Physiology of Nitrate Sensing by Roots

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    Nitrogen (N) is needed by plants in great quantities. Besides being a nutrient, it also acts as a signal, regulating many downstream processes. Understanding the physiological and molecular processes regulating nitrogen use efficiency (NUE), particularly the below-ground traits related to root architecture, is crucial to reducing N loss and improving the efficacy of N fertilisation. Nitrate is the predominant source of nitrogen in aerobic agricultural soils and many studies have investigated the molecular mechanisms underlying the root response to nitrate, especially in Arabidopsis, one of the best studied model plants in plant biology. Maize is a very important crop, and its root apparatus is quite different from and more complex than that of Arabidopsis. Elucidating the molecular events underlying nitrate regulation of the root architecture in both these species is a crucial step towards improving technology transfer in the field. Auxin has been shown to play a prominent role in the transduction process leading to root architecture adjustments in response to nitrate availability in both Arabidopsis and maize, but the two plants differ in many other specific molecular components of this respons

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Nitrate regulates maize root transcriptome through nitric oxide dependent and independent mechanisms

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    Maize root responds to nitrate by modulating its development through the coordinated action of many interacting players. Nitric oxide is produced in primary root early after the nitrate provision, thus inducing root elongation. In this study, RNA sequencing was applied to discover the main molecular signatures distinguishing the response of maize root to nitrate according to their dependency on, or independency of, nitric oxide, thus discriminating the signaling pathways regulated by nitrate through nitric oxide from those regulated by nitrate itself of by further downstream factors. A set of subsequent detailed functional annotation tools (Gene Ontology enrichment, MapMan, KEGG reconstruction pathway, transcription factors detection) were used to gain further information and the lateral root density was measured both in the presence of nitrate and in the presence of nitrate plus cPTIO, a specific NO scavenger, and compared to that observed for N-depleted roots. Our results led us to identify six clusters of transcripts according to their responsiveness to nitric oxide and to their regulation by nitrate provision. In general, shared and specific features for the six clusters were identified, allowing us to determine the overall root response to nitrate according to its dependency on nitric oxide
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