1,721,023 research outputs found
HOPA1’ A NEW PUTATIVE T3SS EFFECTOR OF PSEUDOMONAS SAVASTANOI PV. SAVASTANOI STRAIN DAPP-PG722, THE CASUAL AGENT OF OLIVE KNOT DISEASE
No full textRecently, we reported the draft genome sequence of the Italian DAPP-PG722 strain of Pseudomonas savastanoi pv. savastanoi (Psv), which shows about 85% identity with the Psv genome of the French strain NCPPB3335. Since the Italian strain is less virulent than the French strain in both lignified and young micropropagated olive plants, bioinformatic analysis was performed to verify whether the difference in virulence is dependent on the effector repertoire of the two strains. Results showed that the two strains shared an identical T3SS repertoire, except for a new putative effector, named hopA1’, which is absent in the NCPPB3335 strain. In the DAPP-PG722 Psv genome, the hopA1’ gene is present in an incomplete hrc/hrp gene cluster. Phylogenetic analysis between the amino acid sequence of HopA1’ and those of the HopA1 and HopA2 present in several strains of the P. syringae complex, revealed a high identity of HopA1’ with HopA2 of P. syringae pv. aesculi strain 2250. Dot blot analysis and southern hybridization were carried out to verify the presence of hopA1’ among 35 Psv strains and 26 strains of the P. syringae complex isolated from herbaceous and woody plants. HopA1’ is present in a single copy in the chromosomal DNA of the DAPP-PG722 strain as well as in others 12 Psv strains and 5 strains of the P. syringae complex attacking woody plants. To investigate the role of hopA1’ in Psv virulence the mutant of this gene in the strain DAPP-PG722 and its ectopic expression in NCPPB3335 strain are in progres
AraC-Family Transcriptional Regulator WhpR Controls Virulence in Pseudomonas savastanoi pv. savastanoi Through Regulation of Indole Metabolism
No full text[Data Availability Statement]
RNA-seq data have been deposited in the Sequence Read Archive (SRA) at NCBI under accession number PRJNA1141921. All other data supporting the findings of this study are included in the Supporting Information or are available from the corresponding author upon reasonable request.The Pseudomonas syringae complex WHOP genomic island underpins virulence in woody hosts by mediating the catabolism of aromatic compounds. However, the biochemical functions of the ipoABC and dhoAB operons and the regulatory gene whpR remain unknown. Comparative genomics revealed WHOP-like clusters beyond P. syringae, found in diverse plant-associated, environmental and clinical bacteria, including indole degraders. We propose that ipoABC and dhoAB mediate indole degradation via anthranilate, linking indole detoxification to central metabolism through the β-ketoadipate pathway. In the olive pathogen P. savastanoi pv. savastanoi, ipoABC promotes indole degradation, indigo production, cell aggregation and biofilm formation. WhpR, an AraC-family regulator structurally related to CuxR and ToxT, defines a regulon comprising repression of most WHOP operons along with genes outside this region, including trpAB, reflecting integrated regulation of indole catabolism and tryptophan biosynthesis. In line with the observed transcriptional repression of WHOP genes, deletion of whpR led to hypervirulence and significantly altered bacterial fitness in woody olive plants. These findings define the WHOP region as a regulatory hub linking indole detoxification, multicellular behaviour and virulence, emerging as a target for novel control strategies against woody plant diseases.This research was supported by grant PID2020-115177RB-C21 from the Spanish Ministerio de Ciencia e Innovación (MCIN)/Agencia Estatal de Investigación (AEI)/10.13039/501100011033/, co-financed by the European Regional Development Fund (ERDF) – ‘A way to make EuropePeer reviewe
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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