196,486 research outputs found
Crystal structures of bovine odorant-binding protein in complex with odorant molecules
No full textThe structure of bovine odorant-binding protein (bOBP) revealed a striking feature of a dimer formed by domain swapping [Tegoni, M., Ramoni, R., Bignetti, E., Spinelli, S. & Cambillau, C. (1996) Nat. Struct. Biol.3, 863-867; Bianchet, M.A., Bains, G., Pelosi, P., Pevsner, J., Snyder, S.H., Monaco, H.L. & Amzel, L.M. (1996) Nat. Struct. Biol.3, 934-939] and the presence of a naturally occuring ligand [Ramoni, R., Vincent, F., Grolli, S., Conti, V., Malosse, C., Boyer, F.D., Nagnan-Le Meillour, P., Spinelli, S., Cambillau, C. & Tegoni, M. (2001) J. Biol. Chem.276, 7150-7155]. These features led us to investigate the binding of odorant molecules with bOBP in solution and in the crystal. The behavior of odorant molecules in bOBP resembles that observed with porcine OBP (pOBP), although the latter is monomeric and devoid of ligand when purified. The odorant molecules presented K(d) values with bOBP in the micromolar range. Most of the X-ray structures revealed that odorant molecules interact with a common set of residues forming the cavity wall and do not exhibit specific interactions. Depending on the ligand and on the monomer (A or B), a single residue--Phe89--presents alternate conformations and might control cross-talking between the subunits. Crystal data on both pOBP and bOBP, in contrast with binding and spectroscopic studies on rat OBP in solution, reveal an absence of significant conformational changes involving protein loops or backbone. Thus, the role of OBP in signal triggering remains unresolved
SAT0247 Efficacy and safety of modified-release prednisone in managing moderate activity systemic lupus erythematous during pregnancy: an implemented case-control study
No full textIs Eriocnemis luciani meridae (Aves: Trochilidae) a diagnosable taxon and does it come from Venezuela, with remarks on the collectors Salomón Briceño and Walther Frederick Henninger
No full textKirwan, Guy M., Ramoni-Perazzi, Paolo, Sharpe, Christopher J. (2023): Is Eriocnemis luciani meridae (Aves: Trochilidae) a diagnosable taxon and does it come from Venezuela, with remarks on the collectors Salomón Briceño and Walther Frederick Henninger. Zootaxa 5374 (4): 563-574, DOI: 10.11646/zootaxa.5374.4.7, URL: https://mapress.com/zt/article/download/zootaxa.5374.4.7/5230
Knowledge acquisition based on an epistemological model of medical reasoning
No full textThis paper describes a framework for medical knowledge acquisition able to help a domain expert to build a knowledge-based system. It is based on an epistemological model of medical reasoning and exploits different knowledge representation formalisms
Cossus ramoni – a new species from northeastern Iran (Lepidoptera, Cossidae, Cossinae) /
No full textNew species Cossus ramoni sp. n. is described from northeastern Iran. It is compared with closely distributed Iranian subspecies of Cossus cossus (Linnaeus, 1758) and Cossus afghanistana Daniel, 1953
Influence of additive manufactured stainless steel tool electrode on machinability of beta titanium alloy
No full textAdditive manufacturing technology provides a gateway to completely new horizons for producing a wide range of components, such as manufacturing, medicine, aerospace, automotive, and space explorations, especially in non-conventional manufacturing processes. The present study analyzes the influence of the additive manufactured tool in electrochemical micromachining (ECMM) on machining beta titanium alloy. The influence of different machining parameters, such as applied voltage, electrolytic concentration, and duty ratio on material removal rate (MRR), overcut, and circularity was also analyzed. It was inferred that the additive manufactured tool can produce better circularity and overcut than a bare tool due to its higher corrosion resistance and localization effect. The additive manufactured tool can remove more material owing to its strong atomic bond of metals and higher electrical conductivity
Congenital heart block not associated with anti-Ro/La antibodies: comparison with anti-Ro/La-positive cases.
No full textJ Rheumatol. 2009 Aug;36(8):1744-8. Epub 2009 Jun 30.
Congenital heart block not associated with anti-Ro/La antibodies: comparison with anti-Ro/La-positive cases.
Brucato A, Grava C, Bortolati M, Ikeda K, Milanesi O, Cimaz R, Ramoni V, Vignati G, Martinelli S, Sadou Y, Borghi A, Tincani A, Chan EK, Ruffatti A.
Source
Medicina Interna, Ospedali Riuniti, Bergamo, Italy. [email protected]
Abstract
OBJECTIVE:
To study anti-Ro/La-negative congenital heart block (CHB).
METHODS:
Forty-five fetuses with CHB were evaluated by analysis of anti-Ro/La antibodies using sensitive laboratory methods.
RESULTS:
There were 9 cases of anti-Ro/La-negative CHB; 3 died (33.3%). Only 3 (33.3%) were complete in utero and 5 (55.5%) were unstable. No specific etiology was diagnosed. Six infants (66.6%) were given pacemakers. There were 36 cases of anti-Ro/La-positive CHB. All except 2 infants (94.4%) had complete atrioventricular block in utero. Ten died (27.8%), one (2.7%) developed severe dilated cardiomyopathy, and 26 (72.2%) were given pacemakers.
CONCLUSION:
Nine of the 45 consecutive CHB cases (20%) were anti-Ro/La-negative with no known cause. They were less stable and complete than the anti-Ro/La positive cases.
PMID:
19567621
[PubMed - indexed for MEDLINE]
PMCID: PMC279858
- …
