1,720,992 research outputs found
Sézary's syndrome: a case with blood T-lymphocytes of helper phenotype, elevated IgE levels and circulating immune complexes.
No full textHelper inducer T cells in the lungs of sarcoidosis patients. Analysis of their pathogenic and clinical significance.
No full textPhenotypic analysis of helper CD4+TQ1- cell population, the major helper T-cell subset for B-cell responses, was carried out in BAL fluid of sarcoidosis patients. Most of the BAL CD4+ cells lacked TQ1 membrane antigen. A correlation between the number of helper CD4+TQ1- cells and IgM and IgA levels was observed in 27 sarcoidosis patients' BAL. A role of CD4+TQ1- cells in modulating lung B-cell immunoglobulin secretion in sarcoidosis was confirmed by the fact that BAL IgG level and helper T-cell number correlated well in patients with low-intensity alveolitis. Results showed an inverse correlation between symptom duration and BAL IgM levels and CD4+TQ1- cell number. The number of helper cells was above normal in patients who had symptoms for less than 12 months and within normal range in those who had symptoms for more than that. The pathogenic and clinical relevance of these data is discussed
Alpha-L-Fucosidase activity and isoenzyme characteristics analyzed by chromatofocusing in normal and leukemic lymphocites.
No full textalpha-L-Fucosidase (EC 3.2.1.51) activity and isoenzyme characteristics were analyzed in normal lymphocyte subpopulations, chronic lymphocytic leukemia subpopulations, and acute lymphoblastic leukemia blasts. Similar pH activity profiles revealed that pH 5.0 was optimal in normal and leukemic cells. Unfractionated CLL lymphocytes had a lower specific activity than normal unfractionated lymphocytes (2.5 +/- 1.0 u/10(6) cells v. 4.0 +/- 1.1). CLL B cells and T-cells had lower specific activity than their respective normal counterparts (1.8 +/- 0.2 v. 5.9 +/- 2.0) (B-cells); (2.2 +/- 0.5 v. 3.7 +/- 1.0) (T-cells) suggesting T and B cells in CLL are abnormal. ALL blasts had a higher specific activity compared to unfractionated normal lymphocytes (9.7 +/- 3.0 v. 4.0 +/- 1.1; p less than 0.001). The isoenzyme pattern of normal, CLL and ALL lymphocytes were obtained by automated chromatofocusing on PBE 94 microcolumns using 0.025 M histidine and polybuffer 74. Two major isoenzyme components (B and A) were isolated. The activity ratio of B/A was different in normal, ALL, and CLL cells
Helper inducer T cells in the lungs of sarcoidosis patients. Analysis of their pathogenic and clinical significance.
No full text
Beta-hexosaminidase release by membrane-perturbing agents in normal and leukaemic lymphocites
No full textAlpha-L-fucosidase and beta-hexosaminidase isoenzymes in human leukemia.
No full textalpha-L-Fucosidase and beta-hexosaminidase isoenzyme expressions were studied in normal lymphocytes and granulocytes and their leukemic counterparts. The isoenzyme patterns were obtained by chromatofocusing on PBE 94 microcolumn coupled with automated enzyme assay. In normal and leukemic lymphoid cells, two major alpha-L-Fucosidase isoenzyme components were isolated. PMN, CML, AML and AMMoL revealed an isoenzyme pattern totally different from that seen in lymphoid cells. The two major beta-hexosaminidase forms (A and B) were isolated in normal lymphocytes and granulocytes. In all leukemias tested an high amount of an intermediate form, I, was found. The alpha-L-Fucosidase and beta-hexosaminidase isoenzyme profile may facilitate differentiation between normal and leukemic cells and between different acute leukemias
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