345 research outputs found
Regulation of nitrate uptake and calcium signaling in arabidopsis
Nitrogen (N) is one of the key nutrients required by plants, and its most abundant form, nitrate (NO3-), has been extensively used in fertilizers to improve crop yield. However, this has also led to increased environmental pollution due to leaching and volatilization. Improving nitrogen use efficiency (NUE) is one of the ways to reduce this environmental impact. Nitrogen uptake efficiency (NUpE) is a key component of NUE. The first step in NO3- uptake is the sensing of NO3- signal which was captured through the use of fluorescence imaging. The calcium responses to NO3- as a signal were captured for the two uptake kinetic systems, high-affinity transporter system (HATS) and low-affinity transporter system (LATS). A unique calcium signature was identified in root epidermal cells and root hair cell-specific responses were captured in response to low NO3-. Two potential transcriptional regulators (TFs) were identified through gene regulatory network analysis of NO3--responsive time-series datasets, DIV1 and MYB28, which play an antagonistic role in regulating the expression of the NO3- transporters, NPF6.3 and NRT2.1, and few NO3- assimilation genes. Further characterization revealed the role of DIV1 and MYB28 in altering the functional NO3- transporter activity, highlighting their role as potential candidates for improving NUpE. The regulation of the transporters occurs at the levels of transcription and post-translation. These regulatory processes were captured through individual models, which were combined into an integrated multiscale model describing the NO3- uptake dynamics for NPF6.3. The integrated model confirmed the switch between the uptake kinetic systems, HATS and LATS, to occur at NO3- concentrations above 1 mM and the threshold without incorporating the regulatory aspects was much lower, close to 0.4 mM. Further, at lower external NO3- concentrations, < 0.5 mM, the total assimilated N was more than 50% of the total predicted internal cellular N when using HATS kinetics, compared to only 30% to 40% assimilation when employing LATS mode for NPF6.3. These findings validate the existing knowledge about the NO3- uptake dynamics and open up avenues to study the impact of perturbations in the regulatory mechanisms on NO3- uptake, leading to novel possibilities of improving NUpE.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-05-01The student, Stuti Shrivastava, accepted the attached license on 2021-04-07 at 11:25.The student, Stuti Shrivastava, submitted this Dissertation for approval on 2021-04-07 at 11:50.This Dissertation was approved for publication on 2021-04-08 at 16:42.DSpace SAF Submission Ingestion Package generated from Vireo submission #16245 on 2021-09-16 at 17:02:36Made available in DSpace on 2021-09-17T02:34:20Z (GMT). No. of bitstreams: 3
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Previous issue date: 2021-04-08Embargo set by: Seth Robbins for item 118487
Lift date: 2023-09-17T02:34:57Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemAuthor requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Onl
Big Data: The Next Big Opportunity for Librarians
Big data includes data sets with sizes beyond the ability of commonly used software tools to capture, curate, manage, and process data within a specific time period. Due to its widespread presence in recent times, librarians need to know the basics of Big Data and how it
can impact academic research. The implications of big data are not only limited to research but to daily working of library professionals. The present paper details the concept of big data and how it can be beneficial in the field of librarianship
An investigation of carbon sequestration/ECBM potential in Australian coals: a simulation study for Sydney Coal Basin
A 2002 report by Australian National Greenhouse Gas Inventory suggests that since 1990 Australia's net emissions of carbon dioxide equivalent from stationary combustion sources are on the rise and they are likely to rise even at a higher rate in keeping with continued economic and industrial growth. In order to reduce Australian CO₂emissions, there is a need to identify and explore large-capacity storage locations for CO₂ sequestration. In that context, Australian coal seams, notably the coal-bed methane (CBM) reservoirs in Sydney and Bowen Basins, could potentially be attractive sites to sequester large volumes of greenhouse CO₂ emissions while also recovering the methane gas, a relatively cleaner source of fossil fuel.
This study investigates the deliverability and economic feasibility of CO₂ sequestration through CO₂-Enhanced CBM recovery (CO₂-ECBMR) in the Camden area, Sydney coal basin. The results of the study show that the CO₂-ECBMR impacts the absolute pelmeability of the Camden area significantly. Because of a good reticulated fracture system, the CO₂ breakthrough from the producers is faster. The enhancement of CH₄ recovery by preferential adsorption of CO₂ occurs simultaneously with the abatement of CH₄ recovery by overall decrease in permeability in the CBM reservoir. Hence, the actual CH₄ is produced under the overall effect of these two competing processes, with the operating parameters like producer-injector spacing and injection pressure affecting their relative dominance over each other. The study also addresses the opportunities of a niche for CO₂ sequestration in these coals, which will be dictated mainly by the factors of sequestration economics and status of these coals being "unmineable". The results derived from the study could help the design of an optimum operating strategy in implementing the CO₂ sequestration and enhanced CBM recovery in Sydney Basin, Australia and elsewhere.Thesis (Ph.D.) -- University of Adelaide, Australian School of Petroleum, 200
Comparative study of boosting and bagging based methods for fault detection in a chemical process
Optimum Parameters for Fault Detection in Bioreactor Using Support Vector Machine and Neural Network
Study and Design of a Zero Voltage Switched Boost Converter
We study theoretical circuit operation of zero voltage switching over the basic premise of boost converters (step-up dc chopper circuits). Zero-voltage switching technique is studied which, in contrast to zero-current switching, eliminates the switching loss and dv/dt noise due to the discharging of junction capacitances and the reverse recovery of diodes Zero Voltage Switching (ZVS) including various switching techniques in resonant converters is studied. Also a working model of a Zero Voltage Switched Boost Converter is constructed in the laboratory and its working and waveforms observed
Overview of Microstrip Patch Antenna for GNSS communication System
Microstrip patch antenna are playing very important role in all wireless global navigation satellite communication system. It uses L band, X band, C band, Ku band, Ka band for global navigation satellite system (GNSS) communication application. L band frequency range is 1-2GHz so it can be used in lower frequency range communication. With the increasing need for communication and the emergence of many other systems, it is important to design compact size antennas to cover a wide frequency range. The design of an efficient wideband small size antenna, for recent wireless applications, is a major challenge. Patch antennas have found extensive applications in wireless communication system. In this paper microstrip patch antenna discussed and detail studied of GNSS system application
Acyclic and Macrocyclic Schiff Base-Based Chelating Ligands for Uranyl Ion (Uo22+) Complexation
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