1,721,138 research outputs found

    Study of the C genome QTL affecting flowering time in spring oilseed Brassica napus under a short-day condition

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    Brassica napus, mostly known as canola, is an important oilseed crop in Canada. Earliness of flowering and maturity are one of the important traits for growing spring canola in North America. These traits can be improved through identification of the genes and alleles affecting flowering time and understanding their effect on other traits. In this study, near-isogenic lines (NILs) carrying flowering time QTL alleles of Brassica oleracea or B. napus which located on C1 or C9 chromosome were evaluated under 10 hr photoperiod condition for morphological and root and shoot biomass traits to understand the effect of these QTL alleles on the other traits. The NILs carrying C1 or C9 allele of B. oleracea flowered earlier, as expected, and no visible difference was observed between the NILs and the recurrent parent for leaf characteristics. However, the NILs showed significant difference for plant height, shoot and root biomass indicating that the flowering time QTL alleles may exert pleiotropic effect on these traits or QTL affecting these traits are located in the same genomic region. This thesis research also included expression analysis of putative flowering time genes from C5 and C9 flowering time QTL. For this, a set of early- and late-flowering B. napus lines developed from a B. napus × B. oleracea interspecific cross were used, and expression analysis of 30 flowering genes from C5 (15) and C9 (15) QTL was carried out on leaf and shoot apex tissue collected from the plants grown under 10 hr photoperiod condition. A majority of the genes showed a greater expression at night than in morning, and significant differential expression was observed for CO, PHYA, FT, AP2, SOC1 and TEM1; this suggests that these genes might be involved in flowering time variation between the early and late flowering lines. The leaf materials of the above-mentioned early- and late-flowering plants were also used in a proteomic study to identify the proteins involved in flowering time variation. This study identified the proteins involved in photosynthesis, pigmentation, phytohormone signaling, stress response, ROS, ion binding, mRNA binding, protein binding and many others. Thus, this thesis research showed that the flowering time alleles can exert effect on other morphological and physiological traits, and the research also identified the genes and proteins potentially involved in the regulation of flowering time in canola under short-day photoperiod conditions

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Introgression of clubroot resistance from B. oleracea into B. napus and study the inheritance of the resistance

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    Clubroot disease, caused by Plasmodiophora brassicae, is one of the most devastating threats to Brassica napus canola production worldwide. Growing clubroot-resistant cultivars is considered the most efficient way of managing this disease. All available clubroot-resistant canola cultivars carry resistance in their A-genome. Some of the qualitative resistances located in the A genome of canola became ineffective after growing only for a few years due to the evolution of new P. brassicae pathotypes. This highlighted the need of introducing the quantitative resistance from Brassica oleracea into the C genome of B. napus canola. The objective of this thesis research was to investigate the prospect of developing clubroot-resistant canola quality spring growth habit B. napus plants carrying resistance in the C genome through B. napus × B. oleracea interspecific cross. In addition to this, genetic analysis of clubroot resistance was carried out using canola lines from the canola breeding program to investigate the prospect of developing non-GM (non genetically modified) clubroot resistant canola. This thesis research demonstrated that clubroot resistant euploid B. napus (2n = 38) lines carrying resistance in the C genome can be achieved in advanced generation population of the B. napus × B. oleracea interspecific cross. Mapping of this resistance identified four QTL located on the C genome chromosomes C04 (two loci), C05 and C08. Agronomic and seed quality analysis of the population showed that clubroot resistant B. napus lines that flower a day earlier than the B. napus parent and contain low erucic acid in seed oil and low glucosinolate in seed meal can be achieved from this population. The average oil and protein contents of the advanced generation population was low; however, these traits can be improved through breeding. Segregation for clubroot resistance in the F2 and backcross (B1 = F1 × Resistant parent; B2 = F1 × Susceptible parent) populations derived from clubroot resistant × susceptible canola crosses showed that a major locus involved in the control of resistance in these populations. This simpler genetic control of clubroot resistance suggested that clubroot resistant non-GM canola lines can be developed from these populations without facing much difficulty. Thus, the results from this thesis research demonstrated the feasibility of the development of clubroot-resistant spring growth habit B. napus lines carrying clubroot resistance in the C genome, and disclosed the inheritance of clubroot resistance in three canola populations. The knowledge and materials developed from this thesis research can be used in breeding clubroot resistant canola germplasm/cultivars

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used

    canola

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    UA CountyGold is an imidazoline herbicide resistant open-pollinated non-transgenic spring Brassica napus L. canola cultivar developed from UA BountyGold × UA AlfaGold cross following pedigree breeding method and registered in Canada in 2021. The pedigree of UA CountyGold contains winter canola germplasm. On average, UA CountyGold yielded 3152 kg ha-1. It flowered and matured 4.5-6.5 days later, however, had better straw-stiffness than the WCC/RRC check cultivars. This cultivar had about 0.9% higher oil in seed and 1.5% higher protein in meal, and 0.7% lower saturated fatty acids in the seed-oil than the WCC/RRC checks, and rated as moderately resistant to blackleg disease.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
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