2 research outputs found
Detection of Lumpy Skin Disease Virus in Transhipped Through Merak Port and The Impact of its Spread
No full textResearch to detect the presence of Lumpy Skin Disease Virus (LSDV) was carried out in the work area of the Banten Animal, Fish and Plant Quarantine Center (BBKHIT). Sampling for LSDV detection was taken from cattle that were being transported both in and out of Merak Port. The aim of this research is to detect the presence of LSDV both clinically and in laboratory examinations at Merak Port and estimate the impact of its spread. 152 mouth and nose swab samples were taken, then a quantitative real time PCR (qPCR) test was carried out. The results of the study showed that there were no clinical symptoms observed in the trafficked cattle, however there were 2 positive samples for LSD by molecular testing using qPCR. These results can be said to be subclinically infected cattle. Positive samples came from cattle trafficked from Sumbawa Regency and Central Lampung Regency with respective Ct values of 27.71 and 28.88. Estimates of the impact of the spread of LSDV were carried out based on the categories of breeding cattle and beef cattle. The impact of the spread of LSDV is estimated to be more dangerous and will occur in breeding cattle because of their long lifespan during the rearing process. This means that the LSD virus can continue to spread to other cows rather than cows that are immediately slaughtered.
PENGARUH EKSTRAK KEMANGI (Ocimum sanctum) TERHADAP KEMAMPUAN BELAJAR DAN MEMORI: JUMLAH SARAF NITRERJIK DI SUBIKULUM HIPOKAMPUS TIKUS (Rattus norvegicus)
No full textDegradation of cognitive ability such as memory and learning knows adays
are increasing progressively increasing and occurs in various age groups, not only
in old age but also at the productive age. Holy basil (Ocimum sanctum) is a known
herb which can improve cognitive ability. Cognitive abilities are controlled by
hippocampus. Hippocampus consist of 3 there are dentate gyrus, hippocampus
proper, and subiculum. One of the indication of cognitive ability improvement can
be evaluated from neurotransmitter, one of which is Nitric Oxide (NO). The aim
of research was to determine the influence of holy basil extract agains of cognitive
ability which is evaluated from the number of nitrergic neurons on subiculum.
Nine male rats (Rattus norvegicus) 4 weeks of age used, 50-60 g from Unit
Development of Animal Laboratory of UGM was used on tihs research. Extract of
holy basil (40 mg/kg BW) was given every day per-oral. At 2, 6, and 8 weeks of
treatment 3 rats were anaesthetized using ketamine and xylazine (100 mg/kg and
10 mg/kg), perfused intracardiacally by Nacl 0.9% continued with phosphate
buffer formalin 10%. The brain was taked out and processed for cryostat section
20μm, n stained by hematoxylin Eosin (HE) and Nicotinamide Adenine
Dinucleotide Phosphate-Diaphorase (NADPH-d) method.
Nitrergic neurons on subiculum has pyramidal shape which is have positive
reaction of blue chromatic and the nucleus was not stained. The percentage of
nitrergic neurons in 2, 6, and 8 weeks of treatment are 12,29
