42 research outputs found
Machine learning in hypermedia using digital image content
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Shot-noise limited detection sensitivity in multiplex CARS microscopy
No full textIn multiplex CARS microscopy the generated anti-Stokes signal is generated and detected simultaneously over a significant part of the vibrational spectrum. The signal-to-noise ratio of the thus detected spectra is limited only by shot-noise. This principle is demonstrated using a dilution series of 2-propanol in water. It is derived theoretically and shown experimentally that for low solute concentrations - in contrast to methods that suppress the non-resonant background - the CARS signal strength from a particular vibrational mode depends linearly on its concentration. Furthermore, excellent agreement is shown between the experimental data and fits to the theory. It is shown that this approach permits rapid (20 ms acquisition) detection of a single lipid mono-layer, with sufficient signal-to-noise to determine the order parameter for the acyl chain packing. Also it is demonstrated that this detection scheme provides an absolute measure of the solute concentration.Radiation Chemistry DepartmentApplied Science
ChemInform Abstract: Reaction of the RMgX/Fe(CO)5/CuCl System with Alkynes: Novel Double Carbonylation Leading to Butenolides and Cyclobutenedione.
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Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens
No full textAdaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.Team Raf Van de Pla
Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering
No full textQuantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.Accepted Author ManuscriptImPhys/Quantitative Imagin
Novel Reaction of the [HFe<SUB>3</SUB>(CO)<SUB>11</SUB>]<SUP>-</SUP> reagent with alkynes: a new synthesis of cyclobutenediones
No full textReaction of the [HFe3(CO)11]- species generated in situ using Fe(CO)5 and NaBH4/CH3COOH in THF with alkynes, followed by CuCl2.2H2O oxidation leads to the corresponding cyclobutenediones in 60–73% yields. Reaction of [HFe3(CO)11]- species, generated in situ using Fe(CO)5 and NaBH4/CH3COOH in THF, with alkynes followed by CuCl2·2H2O oxidation leads to the corresponding cyclobutenedione
