97,416 research outputs found
Special features of RAD Sequencing data:implications for genotyping
No full textRestriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop or genotype markers accurately. We show that there are several sources of bias specific to RAD-Seq that are not explicitly addressed by current genotyping tools, namely restriction fragment bias, restriction site heterozygosity and PCR GC content bias. We explore the performance of existing analysis tools given these biases and discuss approaches to limiting or handling biases in RAD-Seq data. While these biases need to be taken seriously, we believe RAD loci affected by them can be excluded or processed with relative ease in most cases and that most RAD loci will be accurately genotyped by existing tools
An investigation into the applicability of the RAD methodology when applied to the development of an information system
No full textWithin any software-related environment many tools, techniques and SDMs (Software Development Methodologies) can be applied to help control the software development lifecycle. The aim of this thesis is to assess the applicability of the RAD (Rapid Application Development) SDM to software development when designing an MIS (Management Information System) for Philips Components Washington. Both positive and negative characteristics of the approach were examined. This thesis provides detailed conclusions on each of the identified characteristics together with more general analysis of the use of SDMs. Research into several SDMs has been carried out in parallel with the development of the MIS, however the RAD SDM was predominantly used throughout the project. This study has been carried out through the analysis of information requirements around the Philips factory with an aim to providing one central system by which all production figures can be collated and reported upon. Prior to this study no standards for the choice of SDM were in place for software design, however previous SDMs used tended to be more traditional and structured in nature. Through this study, additional areas of analysis have been identified in order to further investigate the RAD SDM. Recommendations have also been provided for any future software projects that Philips may undertake with the application of the RAD methodology
Identification of a sex-linked SNP marker in the salmon louse (Lepeophtheirus salmonis) using RAD sequencing
No full textThe salmon louse (Lepeophtheirus salmonis (Krøyer, 1837)) is a parasitic copepod that can, if untreated, cause considerable damage to Atlantic salmon (Salmo salar Linnaeus, 1758) and incurs significant costs to the Atlantic salmon mariculture industry. Salmon lice are gonochoristic and normally show sex ratios close to 1:1. While this observation suggests that sex determination in salmon lice is genetic, with only minor environmental influences, the mechanism of sex determination in the salmon louse is unknown. This paper describes the identification of a sex-linked Single Nucleotide Polymorphism (SNP) marker, providing the first evidence for a genetic mechanism of sex determination in the salmon louse. Restriction site-associated DNA sequencing (RAD-seq) was used to isolate SNP markers in a laboratory-maintained salmon louse strain. A total of 85 million raw Illumina 100 base paired-end reads produced 281,838 unique RAD-tags across 24 unrelated individuals. RAD marker Lsa101901 showed complete association with phenotypic sex for all individuals analysed, being heterozygous in females and homozygous in males. Using an allele-specific PCR assay for genotyping, this SNP association pattern was further confirmed for three unrelated salmon louse strains, displaying complete association with phenotypic sex in a total of 96 genotyped individuals. The marker Lsa101901 was located in the coding region of the prohibitin-2 gene, which showed a sex-dependent differential expression, with mRNA levels determined by RT-qPCR about 1.8-fold higher in adult female than adult male salmon lice. This study's observations of a novel sex-linked SNP marker are consistent with sex determination in the salmon louse being genetic and following a female heterozygous system. Marker Lsa101901 provides a tool to determine the genetic sex of salmon lice, and could be useful in the development of control strategies
12 Kupfertafeln / [rad. v. G. F. Rabe]. [F. W. Meyer sculp.]; [Franz sc.]
No full text12 KUPFERTAFELN / [RAD. V. G. F. RABE]. [F. W. MEYER SCULP.]; [FRANZ SC.]
Die geometrische Zeichnenkunst, oder vollständige Anweisung zum Linearzeichnen, zum Tuschen und zur Construction der Schatten : für Artilleristen, Ingenieure, Baubeflissene und überhaupt für Künstler und Technologen ... / von M. Burg (-)
12 Kupfertafeln / [rad. v. G. F. Rabe]. [F. W. Meyer sculp.]; [Franz sc.] (Th. 2, [Tafelbd.]) (1)
Cover (1)
Tafel I. (2)
Taf. II. (3)
Taf. III. (4)
Taf. IV. (5)
Taf. V. (6)
Taf. VI. (7)
Taf. VII. (8)
Tafel VIII. (9)
Taf. IX. (10)
Tafel X. (11)
Taf. XI. (12)
Taf. XII. (13
Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing
No full textSex in Oreochromis niloticus (Nile tilapia) is principally determined by an XX/XY locus but other genetic and environmental factors also influence sex ratio. Restriction Associated DNA (RAD) sequencing was used in two families derived from crossing XY males with females from an isogenic clonal line, in order to identify Single Nucleotide Polymorphisms (SNPs) and map the sex-determining region(s). We constructed a linkage map with 3,802 SNPs, which corresponded to 3,280 informative markers, and identified a major sex-determining region on linkage group 1, explaining nearly 96% of the phenotypic variance. This sex-determining region was mapped in a 2 cM interval, corresponding to approximately 1.2 Mb in the O. niloticus draft genome. In order to validate this, a diverse family (4 families; 96 individuals in total) and population (40 broodstock individuals) test panel were genotyped for five of the SNPs showing the highest association with phenotypic sex. From the expanded data set, SNPs Oni23063 and Oni28137 showed the highest association, which persisted both in the case of family and population data. Across the entire dataset all females were found to be homozygous for these two SNPs. Males were heterozygous, with the exception of five individuals in the population and two in the family dataset. These fish possessed the homozygous genotype expected of females. Progeny sex ratios (over 95% females) from two of the males with the "female" genotype indicated that they were neomales (XX males). Sex reversal induced by elevated temperature during sexual differentiation also resulted in phenotypic males with the "female" genotype. This study narrows down the region containing the main sex-determining locus, and provides genetic markers tightly linked to this locus, with an association that persisted across the population. These markers will be of use in refining the production of genetically male O. niloticus for aquaculture
RAD-TRAP 2, a program for the solution of the Holstein equation of radiation trapping
No full textAbstract
RAD-TRAP computes the solution of the Holstein equation of radiation trapping for three important geometries: plane-parallel slab, long cylinder, and sphere. The new version 2 offers the direct computation of the steady-state distribution of excited atoms and the computation of the emergent spectra; effects like self-reversal can now be studied. It also includes a new algorithm for a more efficient, highly accurate computation of the cylinder case. The new version also runs on IBM-PCs. ...
Title of program: RAD-TRAP 2
Catalogue Id: ACLB_v2_0 [ACNX]
Nature of problem
Computation of the eigenvalues and eigenfunctions of the Holstein integral equation (describing radiation trapping in an atomic vapor) and the steady-state distribution of excited atoms in one-dimensional geometries.
Versions of this program held in the CPC repository in Mendeley Data
ACLB_v2_0; RAD-TRAP 2; 10.1016/0010-4655(93)90009-2
ACLB_v1_0; RAD-TRAP; 10.1016/0010-4655(93)90108-O
This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2019
RAD-TRAP, a program for the computation of the eigenvalues and eigenfunctions of the Holstein radiation-trapping equation
No full textAbstract
Radiation trapping is described by the Holstein equation, a Fredholm integral equation of the second kind. By combining analytical and numerical techniques, the presented code efficiently computes the eigenvalues and eigenfunctions of this equation for three important geometries: plane-parallel slab, long cylinder, and spherically symmetric geometry; all practically occuring spectral lineshapes are considered. The program is written in standard Fortran.
Title of program: RAD-TRAP
Catalogue Id: ACLB_v1_0
Nature of problem
Computation of the eigenvalues and eigenfunctions of the Holstein integral equation describing radiation trapping in an atomic vapor (in one-dimensional geometries).
Versions of this program held in the CPC repository in Mendeley Data
ACLB_v2_0; RAD-TRAP 2; 10.1016/0010-4655(93)90009-2
ACLB_v1_0; RAD-TRAP; 10.1016/0010-4655(93)90108-O
This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2019
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Investigation the Response of Rapeseed Cultivars to Moisture Regimes in Different Growth Stages
No full textIn order to evaluate the response of rapeseed cultivars to moisture regimes and correlation analysis in different growth stages, a field experiment was conducted in 2006-7 in seed and plant improvement institute of Karaj, Iran. Results showed that number of pods per plant, pod length, stem diameter, 1000-seed weight, oil seed content and branch numbers per plant were reduced significantly under water deficit stress. Also, grain yield was decreased more than biological yield and this resulted in decreased harvest index. Correlation coefficient analysis revealed that number of pods per plant, number of seeds per main and secondary pod, 1000-seed weight, pod length, biological yield and oil seed content had positive and significant correlation with seed yield. Stopping the irrigation from flowering stage also had undesirable effect on seed yield and its components. Among cultivars, Opera cultivar produced more seed yield (4053 kg ha-1) than the others under normal irrigation and under water deficit stress conditions, Zarfam cultivar had the maximum seed yield during stem elongation (3569 kg ha-1), flowering (2135 kg ha-1) and podding stages (2476 kg ha-1). Okapi had the least changes of oil content than other cultivars. Based on the result of this study can be concluded that Zarfam cultivar had better capability to tolerate drought stress and could produce greater seed yield under stress condition
- …
