576 research outputs found
Stem Cells in Cardiovascular Medicine: Historical Overview and Future Prospects
No full textCardiovascular diseases remain the leading cause of death in the developed world, accounting for more than 30% of all deaths. In a large proportion of these patients, acute myocardial infarction is usually the first manifestation, which might further progress to heart failure. In addition, the human heart displays a low regenerative capacity, leading to a loss of cardiomyocytes and persistent tissue scaring, which entails a morbid pathologic sequela. Novel therapeutic approaches are urgently needed. Stem cells, such as induced pluripotent stem cells or embryonic stem cells, exhibit great potential for cell-replacement therapy and an excellent tool for disease modeling, as well as pharmaceutical screening of novel drugs and their cardiac side effects. This review article covers not only the origin of stem cells but tries to summarize their translational potential, as well as potential risks and clinical translation
Architecture, Environment, Teaching and Designing Work in Progress
No full textIl contributo verte sulla descrizione delle fasi metodologiche del Laboratorio di Progettazione Ambientale, nel quale si sono individuati nuovi criteri per la riqualificazione di aree urbane degradate nella città diffusa adriatica.
Il volume raccoglie i diversi contributi delle scuole di architettura europee, in relazione al tema dell'innovazione nel trasferimento delle conoscenze dell'architettura e del progetto urbano sostenibil
Changing Poverty pockets in metropolitans
No full textLa relazione affronta il tema della riqualificazione dei quartieri spontanei dei paesi in via di sviluppo, utilizzando strumenti di rilevazione e di classificazione del costruito per individuare possibili strategie di riqualificazione urbana, a partire dagli spazi di relazione esterni
Micro-RNA 92a as a Therapeutic Target for Cardiac Microvascular Dysfunction in Diabetes
No full textMicrovascular dysfunction is a pathological hallmark of diabetes, and is central to the ethology of diabetes-associated cardiac events. Herein, previous studies have highlighted the role of the vasoactive micro-RNA 92a (miR-92a) in small, as well as large, animal models. In this study, we explore the effects of miR-92a on mouse and human cardiac microvascular endothelial cells (MCMEC, HCMEC), and its underlying molecular mechanisms. Diabetic HCMEC displayed impaired angiogenesis and a pronounced inflammatory phenotype. Quantitative PCR (qPCR) showed an upregulation of miR-92a in primary diabetic HCMEC. Downregulation of miR-92a by antagomir transfection in diabetic HCMEC rescued angiogenesis and ameliorated diabetic endothelial bed inflammation. Furthermore, additional analysis of potential in silico-identified miR-92a targets in diabetic HCMEC revealed the miR-92a dependent downregulation of an essential metalloprotease, ADAM10. Accordingly, downregulation of ADAM10 impaired angiogenesis and wound healing in MCMEC. In myocardial tissue slices from diabetic pigs, ADAM10 dysregulation in micro- and macro-vasculature could be shown. Altogether, our data demonstrate the role of miR-92a in cardiac microvascular dysfunction and inflammation in diabetes. Moreover, we describe for the first time the metalloprotease ADAM10 as a novel miR-92a target, mediating its anti-angiogenic effect
Dysregulation of Krüppel-like Factor 2 and Myocyte Enhancer Factor 2D Drive Cardiac Microvascular Inflammation and Dysfunction in Diabetes
No full textCardiovascular complications are the main cause of morbidity and mortality from diabetes. Herein, vascular inflammation is a major pathological manifestation. We previously characterized the cardiac microvascular inflammatory phenotype in diabetic patients and highlighted micro-RNA 92a (miR-92a) as a driver of endothelial dysfunction. In this article, we further dissect the molecular underlying of these findings by addressing anti-inflammatory Krüppel-like factors 2 and 4 (KLF2 and KLF4). We show that KLF2 dysregulation in diabetes correlates with greater monocyte adhesion as well as migratory defects in cardiac microvascular endothelial cells. We also describe, for the first time, a role for myocyte enhancer factor 2D (MEF2D) in cardiac microvascular dysfunction in diabetes. We show that both KLFs 2 and 4, as well as MEF2D, are dysregulated in human and porcine models of diabetes. Furthermore, we prove a direct interaction between miR-92a and all three targets. Altogether, our data strongly qualify miR-92a as a potential therapeutic target for diabetes-associated cardiovascular disease
Correction: Samak et al. Dysregulation of Krüppel-like Factor 2 and Myocyte Enhancer Factor 2D Drive Cardiac Microvascular Inflammation and Dysfunction in Diabetes. Int. J. Mol. Sci. 2023, 24, 2482
No full textIn the original publication [...
Architecture, Environment, Technologies and New ways of Teaching
No full textIl volume raccoglie contributi delle università europee; sono stati selezionati attraverso una "call for paper" quei contributi che si sono contraddistinti per una particolare innovazione nella didattica e nel trasferimento delle conoscenze riguardo al tema della progettazione ambientale. Il contributo allegato descrive la sperimentazione didattica operata nel Laboratorio di Progettazione Ambientale, orientata a definire una nuova metodologia di indagine dei requisiti ambientali al fine di pervenire ad una progettazione più consapevole e innovativa. Federica Ottone, responsabile del laboratorio di progettazione ambientale, scrive la parte introduttiva del contributo, riferita ai criteri metodologici di impostazione del laboratorio stesso; scrive anche la parte conclusiva che mette in evidenza i risultati attesi, visibili in seguito attraverso alcune esemplificazioni descritte dai dottorandi che hanno collaborato al corso
Urban Lab in Parametric Vision
No full textIl volume raccoglie gli interventi del convegno di Napoli, tenutosi nell'ottobre del 2016, che fa il punto sugli obiettivi della didattica della progettazione ambientale in ambito europeo. In particolare il contributo qui allegato illustra una metodologia didattica di approccio al progetto di trasformazione dello spazio urbano, partendo dall'ipotesi che la relazione tra ambiente naturale, spazi urbani aperti e costruito possa innescare un processo di miglioramento del comfort urbano e della qualità della vita nella città
Interview with Mostafa Moharram
No full textهذه المقابلة مع المؤلف والسيناريست المصري مصطفى محرم. يستعرض كتاباته وأفلامه ، ويؤكد على أهمية السيناريو والكتابات المتخصصة . يشرح دور كاتب السيناريو وهو المسؤول عن خلق عمل فعال وجيد ، وكذلك التعامل بطريقة جيدة مع فريق التمثيل . أجرت المقابلة درية شرف الدينIn this interview, Egyptian author and screenwriter Mostafa Moharram speaks about his movies and the importance of scenarios in creating good work. The interview was conducted by Dorreya Sharaf al-Din
Detection and Analysis of Epilepsy Biomarkers in Electrocorticography
No full textAuthor Mostafa MohammadpourDissertation Johannes Kepler Universität Linz 202
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