197,282 research outputs found
�������Cf-plasma desorption mass spectrometry of chemically protected ribo- and deoxyribooligonucleotides
No full textVita.A new method of determining the base sequence, the purity and the molecular weight of a chemically protected ribo- or deoxyribooligonucleotide is described. Using the method �������Cf-plasma desorption mass spectrometry positive and negative ion mass spectra have been obtained of a variety of protected oligonucleotides ranging from the simplest mononucleotide unit to the tetradecanucleotide level. The �������Cf-PD positive ion spectrum is generally dominated by the presence of molecular ion species in the mass range above 350 M/z. The negative ion spectrum is characterized by the presence of two nested sets of fragment ions formed by the random cleavage of C3'-03' and C5'-05' internucleotidic bonds. Each set therefore contains ions which extend from the 3'- or 5'-terminal mononucleotide to the level of the intact molecular ion. It is thereby possible from an analysis of the �������Cf-PD negative ion spectrum to unequivocably determine the base sequence of the fully protected oligonucleotide. These results have also demonstrated that �������Cf-PDMS can be used to produce and detect ions up to 12,600 M/z
L. Bayle, Rimo Paura ; J. Carner, Encis de Provença ; F. Moutet, L'autro Ribo ; P. Rouquette, De Paraulo e d'Image
No full textM. J. L. Bayle, Rimo Paura ; J. Carner, Encis de Provença ; F. Moutet, L'autro Ribo ; P. Rouquette, De Paraulo e d'Image. In: Bulletin de l'Association Guillaume Budé, n°2, juin 1970. p. 319
Host–Guest Encapsulation of RIBO with TSC4X: Synthesis, Characterization, and Its Application by Physicochemical and Computational Investigations
No full textIn our present work, we synthesized a new encapsulated
complex
denoted as RIBO-TSC4X, which was derived from an important vitamin
riboflavin (RIBO) and p-sulfonatothiacalix[4]arene(TSC4X).
The synthesized complex RIBO-TSC4X was then characterized by utilizing
several spectroscopic techniques such as 1H-NMR, FT-IR,
PXRD, SEM, and TGA. Job’s plot has been employed to show the
encapsulation of RIBO (guest) with TSC4X (host) having a 1:1 molar
ratio. The molecular association constant of the complex entity (RIBO-TSC4X)
was found to be 3116.29 ± 0.17 M–1, suggesting
the formation of a stable complex. The augment in aqueous solubility
of the RIBO-TSC4X complex compared to pure RIBO was investigated by
UV–vis spectroscopy, and it was viewed that the newly synthesized
complex has almost 30 times enhanced solubility over pure RIBO. The
enhancement of thermal stability upto 440 °C for the RIBO-TSC4X
complex was examined by TG analysis. This research also forecasts
RIBO’s release behavior in the presence of CT-DNA, and at the
same time, BSA binding study was also carried out. The synthesized
RIBO-TSC4X complex exhibited comparatively better free radical scavenging
activity, thereby minimizing oxidative injury of the cell as evident
from a series of antioxidant and anti-lipid peroxidation assay. Furthermore,
the RIBO-TSC4X complex showed peroxidase-like biomimetic activity,
which is very useful for several enzyme catalyst reactions
A community-driven roadmap to advance research on translated open reading frames detected by Ribo-seq
No full textRibosome profiling (Ribo-seq) has catalyzed a paradigm shift in our understanding of the translational ‘vocabulary’ of the human genome, discovering thousands of translated open reading frames (ORFs) within long non-coding RNAs and presumed untranslated regions of protein-coding genes. However, reference gene annotation projects have been circumspect in their incorporation of these ORFs due to uncertainties about their experimental reproducibility and physiological roles. Yet, it is indisputable that certain Ribo-seq ORFs make stable proteins, others mediate gene regulation, and many have medical implications. Ultimately, the absence of standardized ORF annotation has created a circular problem: while Ribo-seq ORFs remain unannotated by reference biological databases, this lack of characterisation will thwart research efforts examining their roles. Here, we outline the initial stages of a community-led effort supported by GENCODE / Ensembl, HGNC and UniProt to produce a consolidated catalog of human Ribo-seq ORFs
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States" By M. Carey.
No full text"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States: containing bried sketches of the moral and political character of those states.
By M. Carey, member of the American philosophical, and of the American Antiquarian Society, and author of The Olive Branch, Cindiciae Hibernicae, essays on banking, on political economy, and on internal improvement.
To which are now added the English editor's comments on the subject; together with Important Advice to Emigrants, and Cautions Against Impositions Practiced in the Outports
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Dr. Glendon Swarthout
No full textHosted by Roger M. Busfield, MSU Assistant Professor of Speech and Theater, Meet the Author is designed to introduce a general audience to a contemporary author and their work through in-depth interviews. This episode features a conversation between Dr. Glendon Swarthout, prolific author and English professor at MSU, and assistant professors Sam S. Baskett and Theodore B. Strandness
Differential analysis of Ribo-seq and RNA-seq read counts by genomic feature.
No full textDifferential analysis of Ribo-seq and RNA-seq read counts by genomic feature.</p
Uncovering the small proteome of Methanosarcina mazei using Ribo-seq and peptidomics under different nitrogen conditions
No full textThe mesophilic methanogenic archaeal model organism Methanosarcina mazei strain Gö1 is crucial for climate and environmental research due to its ability to produce methane. Here, we establish a Ribo-seq protocol for M. mazei strain Gö1 under two growth conditions (nitrogen sufficiency and limitation). The translation of 93 previously annotated and 314 unannotated small ORFs, coding for proteins ≤ 70 amino acids, is predicted with high confidence based on Ribo-seq data. LC-MS analysis validates the translation for 62 annotated small ORFs and 26 unannotated small ORFs. Epitope tagging followed by immunoblotting analysis confirms the translation of 13 out of 16 selected unannotated small ORFs. A comprehensive differential transcription and translation analysis reveals that 29 of 314 unannotated small ORFs are differentially regulated in response to nitrogen availability at the transcriptional and 49 at the translational level. A high number of reported small RNAs are emerging as dual-function RNAs, including sRNA154, the central regulatory small RNA of nitrogen metabolism. Several unannotated small ORFs are conserved in Methanosarcina species and overproducing several (small ORF encoded) small proteins suggests key physiological functions. Overall, the comprehensive analysis opens an avenue to elucidate the function(s) of multitudinous small proteins and dual-function RNAs in M. mazei
- …
