1,720,975 research outputs found
Sviluppo vegetativo, produttività e qualità dei frutti di clementine ‘SRA 92’ e arancio ‘Washington navel’ allevati su diversi portinnesti
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Evaluation of alternative splicing in the Citrus sinensis glutathione S transferase gene family
No full textIdentification of differentially expressed genes in the flesh of blood and common oranges
No full textThe objective of this research was the identification
of genes differentially expressed in blood oranges
compared to common oranges and the identification of
anthocyanin pathway genes that are up-regulated in flesh of
blood oranges. A subtracted complementary DNA library
of 1,248 clones was constructed using RNA from the flesh
of a nucellar line (58-8D-1) of Moro (a blood orange) as
tester and from Cadenera (a common orange) as driver.
After screening by reverse Northern, a total of 230 clones
were found to be up-regulated in blood orange, while 30
were up-regulated in the common blond one. Sequence
analysis identified genes involved in the anthocyanin
pathway including genes encoding biosynthetic enzymes
like phenylalanine ammonialyase, chalcone synthase, dihydroflavonol-
4-reductase, anthocyanidin synthase, UDP:
glucose flavonoid 3-O-glucosyltransferase, glutathione
S-transferase, and a regulatory gene encoding a basic Helix-
Loop-Helix protein, while others were related to primary
metabolism, flavor biosynthesis, signal transduction mechanisms,
and defense. Some sequences were classified as
unknown and unnamed and some others were unclassified.
Semiquantitative reverse transcription-polymerase chain
reaction (PCR) and quantitative real-time PCR were used
to confirm the differential expression patterns of selected
candidate genes of different functional classes. Correlations
between the expression of some genes and the processes
involved in the ripening of blood oranges were identified
Horticultural evaluation of new Citrus latipes hybrids as rootstocks for Citrus
No full textIn 1968, the CRA-Research Center for Citriculture and Mediterranean Crops (CRA-ACM) started a research program aimed at breeding citrus rootstocks. Thespecies C. latipes (Swing.) Tan. was used as the female parent; trifoliate [Poncirus trifoliata (L.) Raf.], sour orange, and volkamer lemon (C. volkameriana .) were used as male parents. The behavior of some of these hybrids tested with other rootstocks in Sicily and Sardinia was evaluated. The cultivars under comparison 'Washington' navel orange and 'SRA 92' clementine in Sardinia and 'Tarocco' orange in Sicily. Our results showed the dramatic influence of rootstock ongrowth and yield; only minor effects on fruit quality were observed. Among therootstocks tested, Swingle citrumelo provided the highest yield. Some of the hybrids (F5 P12, F6 P12, and F6 P13) may improve plant yield, thus maintainingfruit quality. Encouraging data obtained with these hybrids may justify the use of monoembryonic species of the Papeda subgenus for breeding citrus rootstocks
Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening
No full textChanges in anthocyanin content and antioxidant activity during ripening of different citrus genotypes.
No full textAnaysis of sweet orange flesh proteome at ripening time and comparison with trascriptomic data
No full textRootstock–scion interaction affecting citrus response to CTV infection: a proteomic view
No full textCitrus tristeza virus (CTV) is the causal agent of various diseases with dramatic
effects on citrus crops worldwide. Most Citrus species, grown on their
own roots, are symptomless hosts for many CTV isolates. However, depending
on different scion–rootstock combination, CTV infection should result in
distinct syndromes, being ‘tristeza’ the more severe one, leading to a complete
decline of the susceptible plants in a few weeks. Transcriptomic analyses
revealed several genes involved either in defense response, or systemic
acquired resistance, as well as transcription factors and components of the
phosphorylation cascades, to be differentially regulated during CTV infection
in Citrus aurantifolia species. To date little is known about the molecular
mechanism of this host–pathogen interaction, and about the rootstock
effect on citrus response to CTV infection. In this work, the response to CTV
infection has been investigated in tolerant and susceptible scion–rootstock
combinations by two-dimensional gel electrophoresis (2DE). A total of 125
protein spots have been found to be differently accumulated and/or phosphorylated
between the two rootstock combinations. Downregulation in tolerant
plants upon CTV infection was detected for proteins involved in reactive oxygen
species (ROS) scavenging and defense response, suggesting a probable
acclimation response able to minimize the systemic effects of virus infection.
Some of these proteins resulted to be modulated also in absence of virus
infection, revealing a rootstock effect on scion proteome modulation. Moreover,
the phospho-modulation of proteins involved in ROS scavenging and
defense response, further supports their involvement either in scion–rootstock
crosstalk or in the establishment of tolerance/susceptibility to CTV infection
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