1,721,086 research outputs found
Synthetic biology: From description of, to the synthesis of, livings
No full textSynthetic biology is aimed to synthetize new livings (i.e. self-replicating) taking advantage of the theorethical grounds of system biology to-artificially reproduce some of the onthological steps of the livings and - to isolate, to transfer, to synthetize single living components. Synthetic biology is a constitutive field of the convergent technologies. It is now-a-day employed to produce new molecules, biofuel and food, photosynthetic microorganisms able to double their biomasses in a few hours, cybrids cells and biologically inspired informatic systems among many other manufactures (goods). © 2012 Elsevier srl
Isolation and characterization of mouse antral oocytes based on nucleolar chromatin organization
No full textThis protocol describes a simple and quick method to isolate and characterize mouse antral GV (Germinal Vesicle) oocytes as able (SN, Surrounded Nucleolus) or unable (NSN, Not Surrounded Nucleolus) to develop to the blastocyst stage after in vitro maturation (IVM) and in vitro fertilization (IVF). It makes use of Hoeschst33342 (or any other DNA intercalating dye) able to bind to the heterochromatin of the nucleolus showing a ring in the SN oocytes or not, like in the NSN oocytes. This represents the easiest and quickest way to sort both antral oocytes that can be eventually used for IVM or IVF procedures. Briefly, the protocol consists of the following steps: hormone injection to stimulate follicular growth; isolation of the oocytes at the GV stage from the antral compartment by puncturing the ovary with a sterile needle; preparation of thin glass pipettes for mouth pipetting of the oocytes; sorting of the oocytes with Hoechst33342 prepared at a supravital concentration; IVM, IVF or any other molecular/cellular analysis. Unfortunately there are still few evidences to sort SN and NSN oocytes using less invasive techniques. If and once they will be identified, they could be potentially applied to human assisted reproductive technologies, although with several aspects that should be modified. To date, this technique has potential implications to dramatically increase IVM and IVF successful procedures in both endangered and species with economic interest
Nuclear architecture of germ and Sertoli cells in homozygous and heterozygous mice for Rb chromosomes
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