1,614 research outputs found
Structural studies of cytidine repressor and catabolite activator protein
No full textThe Escherichia Coli Catabolite Activator Protein (CAP) activates DNA transcription at more than a hundred promoters. Cytidine Repressor (CytR), in conjunction with two CAP dimers, acts as a repressor of DNA transcription at the deoP2 promoter. In the first part of this work, we describe a method by which a (CAP)[subscript] 2-CytR-DNA complex can be prepared for structural studies. In the second part of this project, we describe the crystallization of what was initially
intended to be a (CAP)[subscript] 2-DNA complex in order to study the effects of two CAP dimers on transcription at an artificially constructed promoter containing tandem CAP binding sites. Upon structure determination of the crystal, we observed that while there was no DNA present, the protein had bound multiple Co[superscript]2+ and SO2−/4 ligands. We provide an analysis of the crystal structure and present a possible explanation for the absence of DNA in the structure.M.S.Includes bibliographical referencesby Ramya Rangesh Ra
Knot theory addendum
No full textAt Right Angles met up with author Ramya to discuss her article on Knot Theory. Over coffee at Starbucks, Ramya adeptly made sense of a tangled bunch of wool which I had carried with me to try and see if Knot Theory could help me untangle the web
Water mass classification using band ratios
No full textThe Hudson River plume has been the topic of consideration and observation in order to try and understand the physical, chemical and biological behavior of the plume which is a key component of the oceanography of the Mid-Atlantic Bight (MAB) region off the east coast of the United States. One approach towards understanding the chlorophyll production for the plume would be to make use of satellite data to measure the optical ocean color properties of these waters. In this direction classifying the water masses of the Hudson River plume according to these optical properties would be an interesting method of analyzing the satellite data for the purpose of understanding and identifying the physical and biological changes and the correlation between them in this region.
The first step is to design and implement a water mass classification algorithm in the LaTTE (Lagrangian Transport and Transformation Experiment) region of the MAB. It takes about 1-2 weeks for the nutrients from the freshwater from the Hudson Estuary to be dissipated and mixed with the open ocean. This classification algorithm is developed using ocean color data from the Sea viewing WIde Field of view Sensor (SeaWiFS). The algorithm is validated by overlaying ship salinity tracks on the classified water masses to show that salinity values change at the boundaries of the classified regions, due to the mixing and export of freshwater across the shelf.
We analyze global Sea Surface Temperature (SST) data collected over the years 1995-2005 for summer and winter in order to find coastal estuarine ecosystems that may display similar behavior as the Hudson River Estuary. Looking at the seasonal variation in this data, we observe that the regions of MAB and the East Asian coast are found to have strikingly similar seasonal behavior.
This leads into the third and the last step of the process which involves applying the water mass classification algorithm to ocean color data from eastern coastal Asia. It is observed that the algorithm well in the seas of Okhotsk, Japan and East China where it is able to identify plume water and non river water.M.S.Includes bibliographical references (p. 86-92)
Supplementry_Information - Study on the effect of silica–graphite filler on the rheometric, mechanical, and abrasion loss properties of styrene–butadiene rubber vulcanizates
No full textSupplementry_Information for Study on the effect of silica–graphite filler on the rheometric, mechanical, and abrasion loss properties of styrene–butadiene rubber vulcanizates by AM Shanmugharaj, K Thileep Kumar, G Sivagaami Sundari, E Senthil Kumar, A Ashwini, M Ramya, P Varsha, R Kalaivani, S Raghu, and SH Ryu in Journal of Elastomers & Plastics</p
Development and psychometric testing of a visual analog scale for dental fluorosis
No full textObjectives: The aim was to develop and psychometrically test a visual analog scale (VAS) for dental fluorosis. Materials and Methods: In a cross-sectional study, esthetic perceptions of varying severities of fluorosis in permanent maxillary incisor teeth presented in 36 photographs were graded on a 10 cm VAS by two groups - an expert panel of dental specialists and lay group comprising 1 st year dental students. The photographs were also graded using Thylstrup-Fejerskov index (TFI). Inter and intra-examiner reliability testing and comparison of specialists′ and 1 st year students′ perceptions were made using intra-class correlation coefficient (ICC). Mean VAS values were validated against TFI scores using Spearman correlation coefficient. Results: A VAS for dental fluorosis was constructed with the help of mean values of subjective ratings for each of the photographs. Inter-rater reliability was found to be moderate (ICC = 0.59). The intra-rater reliability for both the lay group (ICC range: 0.91-0.98) and dental specialists′ group (ICC range: 0.87-0.98) was found to be excellent. High correlation was obtained between lay group′s and dental specialists′ perception of fluorosis on VAS (r = 0.93). VAS scores correlated moderately with TFI scores for the photographs (Spearman′s ρ = 0.725). All the findings were found to be statistically significant (P < 0.05). Conclusion: The developed VAS was found to be a valid and reliable tool to assess dental fluorosis. Validation of the scale on a larger sample from the general population is recommended before it is applied in clinical and epidemiological settings
Supplemental Material, Supplementary_Material1 - A Randomized Controlled Trial of Strategies to Improve Family Members’ Preparedness for Surrogate Decision-Making
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Supplemental Material, Supplementary_Material1 for A Randomized Controlled Trial of Strategies to Improve Family Members’ Preparedness for Surrogate Decision-Making by Michael J. Green, Lauren J. Van Scoy, Andrew J. Foy, Renee R. Stewart, Ramya Sampath, Jane R. Schubart, Erik B. Lehman, Anne E.F. Dimmock, Ashley M. Bucher, Lisa S. Lehmann, Alyssa F. Harlow, Chengwu Yang, and Benjamin H. Levi in American Journal of Hospice and Palliative Medicine®
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A study on coping patterns of junior college students
No full textThe objective of this study was to examine the coping patterns followed by the junior college students. Further, an extensive effort was done to study the gender differences in coping patterns used by the students. This study was conducted in Christ College, Bangalore and on the first and second-year students of pre-university studying in either of the branches (Bachelor of Arts, Science, or Commerce). A total of 120 samples were collected from study population of junior college students using the random sampling method. The sample comprised, 40 students from each group of Arts, Science, and Commerce, including both of the sexes. The tools such as, socio-demographic data sheet and coping checklist, were used. The study findings revealed that majority of the students adopted emotion- and problem-focused coping strategies. Most of the female students adopted emotion-focused coping strategies, whereas the male students mostly used problem-focused coping strategies
Supplemental Material, Supplementary_Material3 - A Randomized Controlled Trial of Strategies to Improve Family Members’ Preparedness for Surrogate Decision-Making
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Supplemental Material, Supplementary_Material3 for A Randomized Controlled Trial of Strategies to Improve Family Members’ Preparedness for Surrogate Decision-Making by Michael J. Green, Lauren J. Van Scoy, Andrew J. Foy, Renee R. Stewart, Ramya Sampath, Jane R. Schubart, Erik B. Lehman, Anne E.F. Dimmock, Ashley M. Bucher, Lisa S. Lehmann, Alyssa F. Harlow, Chengwu Yang, and Benjamin H. Levi in American Journal of Hospice and Palliative Medicine®
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Supplemental Material, Supplementary_Material4 - A Randomized Controlled Trial of Strategies to Improve Family Members’ Preparedness for Surrogate Decision-Making
No full text
Supplemental Material, Supplementary_Material4 for A Randomized Controlled Trial of Strategies to Improve Family Members’ Preparedness for Surrogate Decision-Making by Michael J. Green, Lauren J. Van Scoy, Andrew J. Foy, Renee R. Stewart, Ramya Sampath, Jane R. Schubart, Erik B. Lehman, Anne E.F. Dimmock, Ashley M. Bucher, Lisa S. Lehmann, Alyssa F. Harlow, Chengwu Yang, and Benjamin H. Levi in American Journal of Hospice and Palliative Medicine®
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PRONIOSOMES - A CARRIER FOR NOVEL DRUG DELIVERY SYSTEM: A REVIEW
No full textNivya P.*, Dr. S. Daisy Chella Kumari, Dr. R. Devi Damayanthi, Ramya Selvam T. and Bhuvaneswari K
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