1,720,982 research outputs found

    Computer assisted evaluation of IEF patterns in electrophoretic gels : identification of Smoothhounds (Mustelus mustelus, Mustelus asterias) and comparison with lower value sharks

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    In this work, the most commercially important Selachian species, smooth-hound (Mustelus mustelus) and starry smoothhound (Mustelus asterias), have been identified by polyacrylamide isoelectric focusing (IEF-PAGE), along with several shark species of minor commercial value. In Italy, these two smoothhound species are commonly subjected to fraudulent substitution with lesser valued sharks. After the electrophoretic runs, the band patterns of the two Mustelus species were compared with those of dogfish (Scyliorhinus canicula), spurdog (Squalus acanthias), blue shark (Prionace glauca) and black-mouthed dogfish (Galeus melanostomus), both visually and with gel analysis software. The actual isoelectric points were then submitted to cluster analysis to differentiate the single species, despite the possible occurrence of electrophoretic variations or protein polymorphism. Every shark showed species-specific band patterns and could therefore be well differentiated, as confirmed by statistical analysis

    Isoelectric focusing of sarcoplasmic proteins to distinguish swordfish, blue marlin and Mediterranean spearfish

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    In the present study we show that the method of isoelectric focusing (IEF) is a simple and reliable one for detecting the fraudulent substitution of cold-smoked fillets of swordfish (Xiphias gladius) with those of lower value blue marlin (Makaira mazara) and for detecting substitution of blue marlin steaks with those of Mediterranean spearfish (Tetrapturus belone). The cold smoking process does not alter the sarcoplasmic proteins of the fish species, so cold smoked fillets of the species investigated can be identified in the same was as fresh and frozen samples

    Determinazione di piombo e cadmio in molluschi cefalopodi del Mediterraneo

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    The aim of this work was to measure concentration of total Lead and total Cadmium in cephalopods from various areas of the Mediterranean Sea; for each species and areas, three specimens with the same weight were analysed. The two heavy metal were measure by potentiometric stripping unit (PSU20 Trace Lab). All samples had a concentration of the two metals lower than the legal limits. The hightest value of lead was found in flying squid (Todarodes sagittatus) from Sanremo. The hightest value of cadmium was found in flying squid (Todarodes sagittatus) from Bari

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Use of compositional analysis to distinguish farmed and wild gilthead seabream (Sparus aurata)

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    Recent food scares have emphasized the need for traceability in the food chain. This is particularly true in the fish sector, where a large difference in the final price between wild and farmed fish and even between farmed fish reared in different countries exists. So for these commercial and hygienic reasons it is very important to find useful tools for the characterization of quality of fish and for the differentiation of fish from different production methods and from different countries, in accordance with EC Regulation No. 2065/2001 and Italian Ministry of Agriculture Decree No. 27.03.02, that allow retail trade of fishery and aquaculture products only against the indication of the official commercial name, the method of production and the geographical origin of fish (Moretti et al., 2003)

    Determinazione del contenuto naturale di acidi organici in platessa (Pleuronectes platessa)

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    This work describes a simple, rapid, and reliable HPLC method for the determination of organic acids in European plaice (Pleuronectes platessa). The natural concentrations of lactic, acetic and citric acids in whole European plaice were quantified by HPLC system with UV detection, the mean and the 95% confidence interval of each organic acids were calculated. The mean concentration of lactic acid, citric acid and acetic acid were respectively 2596,0 ppm, 1110,8 ppm and 640,5 ppm. The method was applied to fresh fillets of European plaice with and without the statement of citric acid added in label. Two samples, without statement of added additives in label, had a citric acid concentration over the right side of confidence interval of mean calculated. In conclusion, the proposed method is suitable for the control of the presence of organic acid added
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