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    PHYSIOLOGICAL AND ECOLOGICAL DRIVERS AND AGRONOMICAL CONSEQUENCES OF THE OZONE-LIKE SYNDROME IN WHEAT

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    Tropospheric ozone (O3) is known to adversely affect the productivity of a wide range of crops including wheat. However different species, can exhibit different responses to ozone exposure. Since the Triticum genus (wheat) is one of the most cultivated and consumed cereal on the global scale and also considered an O3-sensitive crop, research on its protection against ozone damages can contribute to the improvement of its productivity and thus the worldwide food security. The first part of this research was focused on a varietal screening experiment carried out in 2013 to assess the ozone sensitivity of 3 Italian and 2 Spanish cultivars of durum wheat (Triticum durum), applying two different levels of ozone (50% increment and 50% decrement of the ambient ozone concentration ) to plants grown in Open-Top Chambers. The durum wheat sensitivity to ozone was based on the assessment of leaf visible injuries, histochemical observations, physiological parameters, yield and yield quality analysis. Two Italian cultivars (Colombo and Sculptur) resulted more sensitive to ozone than the others, according to the physiological parameters tested and to the grain yield and quality analysis. However, they also showed different levels of leaf visible and microscopic injuries. The flag-leaves of cv Colombo resulted clearly more damaged by ozone exposure than the flag-leaves of cv Sculptur at both visible and microscopic levels. The second part of the research tried to analyze to a deeper extent the response to ozone exposure of the two most sensitive cultivars selected from the previous experiment. A second experiment was performed in 2014 using four ozone levels: -5% and -50% of ambient ozone concentration in non-filtered and charcoal-filtered OTCs, respectively; +30 and +60% of ambient ozone concentration in ozone-enriched OTCs (OZ+ and OZ++ OTC). In order to test the effectiveness of an antitranspirant compound in protecting durum wheat from ozone oxidative stress, a chitosan solution was weekly applied as leaf spraying during the growing season in 2014. The chitosan treatments were applied at 3 levels: tap-water (CTRL, no chitosan), 40kDa chitosan solution (CHI40) and 300kDa chitosan solution (CHI300). Both durum wheat cultivars confirmed their sensitivity to ozone as observed in the previous experiment. Grain yield losses observed in ozonated treatments were related to a decrease of stomatal conductance that is due to damages to the Rubisco and Calvin cycle. No protective effect due to chitosan treatments was observed in both cultivars. However, chitosan improved slightly the grain yield and the aboveground biomass production in plants grown in charcoal-filtered and non-filtered OTCs. Biomass data were also used for the definition of dose-effect relationships based on the ozone exposure (AOT40), the phytotoxic ozone dose (POD6) and the yield losses. The grain yield losses were plotted against AOT40 and POD6 in order to test the linear regression of these two indices. Each increase of AOT40 3000 ppb.h caused a grain yield loss of about 1.8%, while for the POD6, an increase of 1mmol O3 m-2 caused 1.3% reduction. Both AOT40 and POD6 resulted appropriated for assessment of durum wheat yield losses. However, the dose-effect relationship based on POD6 showed a better fit compared to the AOT40. During the 2014 experiment an important part of the research regarded the ultrastructural analysis of ozone-like symptoms on flag-leaves carried out by transmission electron microscopy (TEM), and the assessment of the levels of some antioxidant molecules (ascorbate and glutathione) involved in the ozone-detoxifying process, to understand the mechanisms underlying the different ozone sensitivity of Colombo and Sculptur in terms of visible and microscopic symptoms. Results from TEM demonstrated that visible symptoms in Colombo are due to the presence of damaged stomata and plasmolyzed mesophyll cells around the sub-stomatal cavity. On other hand, no damage on stomata, mesophyll cells and chloroplasts were observed in Sculptur cultivar explaining the absence of the visible symptoms. In general Sculptur showed higher levels of ascorbate content than Colombo, suggesting a higher capacity ascorbate biosynthesis. No significant difference in ascorbate content was found between plants exposed and not exposed to elevated ozone. The total and the oxidized glutathione content increased in the Colombo cultivar grown in elevated ozone conditions indicating that plant ability to maintain glutathione in the reduced form was decreased by the ozone stress

    Ozone dose-response relationships for durum wheat in Mediterranean conditions

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    The aim of this study was to define an ozone (O3) dose-response function and identify a critical level for the protection of Triticum durum in Mediterranean conditions. ‘Colombo’ and ‘Sculptur’ are two modern durum wheat cultivars that in previous studies proved to be very sensitive to O3 stress at both eco-physiological and agronomical level. Two consecutive experiments on these cultivars were carried out in 2013 and 2014 at the Open-Top Chambers facility of Curno (Northern Italy). Plants of durum wheat were exposed to 2 and 4 different levels of O3 in 2013 and 2014, respectively. The seasonal accumulation of Phytotoxic Ozone Dose (as POD6) and O3 exposure (as AOT40) were correlated with the reduction of grain yield, total aboveground biomass, stems, number of spikes and hectolitre weight. ‘Colombo’ resulted more affected by O3 than ‘Sculptur’ in both years of the experiments, with a significant decrease in yield and growth parameters. ‘Sculptur’ showed significant negative effects only in the highest O3 level treatments. Regression analysis on grain yield were performed using both the AOT40 and the POD6, and the relative effects were calculated on the basis of the mean values of plants grown in Charcoal-Filtered OTC (-50% of ambient ozone). According to this study the POD6 value causing a 5% of decrease in relative grain yield was around 3 mmol O3 m-2 for cv ‘Colombo’ and 4 mmol O3 m-2 for cv ‘Sculptur’. Considering the two cultivars together, we can propose a critical level of POD6 of 3.5 mmol O3 m-2 for a 5% reduction of grain yield to be used in the Mediterranean countries for Triticum durum. Analogously the AOT40 critical level could be set to 8’000 ppb.h. The critical level based on POD6 is 3.5 times higher than that proposed in the Mapping Manual for Triticum aestivum (1 mmol O3 m-2). Results of this study demonstrate clearly that both relationships based on the O3 exposure and POD6 proposed in the Mapping Manual could overestimate the O3 effects on durum wheat under Mediterranean conditions

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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