511 research outputs found
Labrecque House Photograph
No full textPhotograph of the Labrecque family at their home the day of the Easter Parade in Lewiston, Maine, 1961.
L to R: Mrs. Labrecque [Mom], Paul Labrecque, Roger Labrecque, Victor Labrecque
Photograph by Victor Labrecquehttps://digitalcommons.usm.maine.edu/labrecque-wedding-photographs/1004/thumbnail.jp
Free for All: An Open Source Online Based Teaching Template.
No full textBates, R., Young, K.B., LaBrecque, B., Southard, S. (2020,Dec) Free For All: An Open Source Online Based Teaching Template. Published in the Online Journal of Distance Learning Administration (OJDLA
Free for All: An Open Source Online Based Teaching Template.
No full textBates, R., Young, K.B., LaBrecque, B., Southard, S. (2020,Dec) Free For All: An Open Source Online Based Teaching Template. Published in the Online Journal of Distance Learning Administration (OJDLA
Free for All: An Open Source Online Based Teaching Template.
No full textBates, R., Young, K.B., LaBrecque, B., Southard, S. (2020,Dec) Free For All: An Open Source Online Based Teaching Template. Published in the Online Journal of Distance Learning Administration (OJDLA
Free for All: An Open Source Online Based Teaching Template.
No full textBates, R., Young, K.B., LaBrecque, B., Southard, S. (2020,Dec) Free For All: An Open Source Online Based Teaching Template. Published in the Online Journal of Distance Learning Administration (OJDLA
Bovine Oocyte Transcriptome in Relation to Chromatin Configuration of the Germinal Vesicle
No full textVarious physiological contexts have been used to study the mRNA composition of bovine oocyte in relation with the potential to reach the blastocyst stage. The chromatin configuration of the oocyte at the germinal vesicle (GV) stage has been positively associated with developmental competence, where a more compacted chromatin is correlated with a better chance to get an embryo. Four distinct and progressive states of chromatin condensation can be observed and are linked with the sequential acquisition of the developmental potential in bovine species. Therefore, GV stage oocytes were collected and separated in four groups according to their degree of chromatin condensation. Transcriptomic analyses were performed with the EmbryoGENE microarray platform in order to identify transcripts modulations during the gradual chromatin remodeling. A reference design was used where GV0 stage (diffused chromatin state) was compared with the other three groups (GV1; GV2 and GV3, the latter refers to the condensed chromatin state). The number of transcripts significantly different was relatively similar between the three contrasts (an average of 300 genes, fold change > 2; p-value < 0.01) with the majority of them were presenting a reduced level in oocyte with more compacted chromatin. However, among the transcripts presenting an increased mRNA level in these contrasts, many of them are associated with histone genes, chromatin organization and transcriptional regulation. These results suggest that even in a context of transcriptional repression, there is still an accumulation of specific transcripts. Adequate storage of specific histone mRNAs could provide an optimal maternal accumulation in order to fulfill the requirement for the subsequent cell divisions until embryonic genome activation. This dataset will provide a more complete characterization of the oocyte transcriptome modulations and will help us to better understand the competence acquisition in the oocyte at the end of the follicular growth
L'anthropologie du développement au temps de la mondialisation
No full textRÉSUMÉL'anthropologie du développement au temps de la mondialisationÉcrit du point de vue de l'économie politique féministe et basé sur une expérience ethnographique en Amérique latine (surtout au Mexique), cet article porte sur l'anthropologie du développement et son objet, la relation de développement. Le premier objectif de l'article est d'effectuer un retour sur les contributions récentes de différents auteurs dans le domaine et de cerner l'objet de l'anthropologie du développement dans le contexte des processus de mondialisation. Le deuxième objectif est de faire ressortir l'aspect «genre» du développement et de la restructuration économique, tout en faisant le lien avec la question de l'État. Enfin, l'auteure montre que l'approche méthodologique de la relation de développement gagnerait à envisager son déploiement selon différents champs du social.Mots clés : Labrecque, anthropologie du développement, mondialisation, genre, État, économie politiqueABSTRACTDevelopment Anthropology in Times of GlobalizationWritten from a feminist political economy standpoint and with a Latin American ethnographie experience in mind (especially Mexico), this article deals with development anthropology and its main concern, the study of development as a social relation. On the one hand, it takes into account récent contributions on the subject and shows how différent authors revisited development as a social relation in the context of globalization. On thé other hand, the article seeks to show the gendered aspect of development and economie restructuring while discussing the question of the state. Finally, from a methodological point of view, the author argues that development as a social relation should be dealt with in different social arenas.Key words : Labrecque, development anthropology, globalization, gender, state, political econom
Gene expression profile of cumulus cells investment of bovine oocytes at different stage of differentiation based on the degree of their chromatin compaction
No full textOocytes acquire their competence during antral follicle development. During this period, chromatin configuration within the germinal vesicle (GV) changes dynamically and is indicative of oocyte’s developmental potential. Since interactions between somatic and germ cells are critical for the acquisition of oocyte competence and serve modulate chromatin morphology and function, we hypothesized that features of cumulus cells (CC) investment change along with oocyte chromatin compaction, reflecting oocytes quality. Therefore this study aimed at assessing gene expression profile by microarray platform of CC isolated from oocytes with different chromatin configurations
Methods
Bovine cumulus-oocyte complexes were isolated from 0.5-6 mm antral follicles. Oocytes were separated from surrounding CC and chromatin configuration was assessed under fluorescent microscopy. Oocytes were classified as GV0, GV1, GV2 and GV3 according to the degree of chromatin compaction. RNA extracted from CC belonging to each group was, amplified and hybridized on a bovine embryo-specific 44K Agilent slide (EmbryoGene). The CC_GV1, CC_GV2 and CC_GV3 classes were each hybridized against the CC_GV0, representing a stage of early oocyte differentiation with poor development competence. Data were normalized with Loess and fold
changes of differentially expressed genes were determined with Limma procedure. Microarray data were validated using quantitative RT-PCR on selected targets. Microarray data were further analysed through: 1) between- group analysis (BGA), which classifies the samples according to their transcriptomic profiles; 3) cluster analysis according to the expression profile of each gene using the mFuzz Bioconductor package; 4) Ingenuity Pathway Analysis (IPA) to study regulation pattern genes and predicted function. Finally CC belonging to each GV group were cultured for 3hrs and apoptotic cells were assessed by CaspaTag Pan-Caspase in situ Assay.
Results
Overall, qRT-PCR results of a subset of 5 selected genes (Thrombospondine- 1, Serpine-2, regulator of G-protein signaling-2, inhibin alpha and solute carrier family 39 member-8) were consistent with the microarray data.
Clustering analysis generated 16 clusters representing the main profiles of transcription modulation. Of the 5571 significantly differentially expressed probes, the majority (25.49%) best fitted with cluster #6 (down regulation between CC_GV0 and CC_GV1 and stable low levels in successive groups). IPA identified the most relevant functions associated to each cluster. Genes included in cluster #1 were mostly related to biological processes such as ‘cell cycle’ and ‘cell death and survival’, while genes included in cluster #5 were mostly related to ‘gene expression’. Interestingly, ‘lipid metabolism’ was the most significant function identified in cluster # 6, #9 and #12.
IPA of gene lists obtained from each contrast individually (CC_GV0 vs CC_GV1; CC_GV0 vs CC_GV2; CC_GV0 vs CC_GV3), which considered the fold change difference for each gene, revealed that the main affected functions in each contrast was ‘cell death and survival’. Importantly, IPA revealed that apoptosis is predicted to be inhibited in CC_GV1 and CC_GV2, while it is activated in CC_GV3.
Caspase Assay results indicated that a low percentage of CC_GV0 are prone to undergo apoptosis, while it significantly increases in CC from oocytes with condensed chromatin, reaching the highest level in CC_GV3 (ANOVA, p<0.05).
Conclusions
This study confirms that oocyte competence is related to the transcriptomic profile of somatic compartment and provides multiple non-invasive biomarkers that can predict oocyte quality. This has important implications in treating human infertility as well as developing breeding schemes in domestic mammals
Figure 1 from: Lambert H, Fortin G, Labbé R, Labrecque J, Bérubé JA, Landry J, Ilyukhin E, Margaritescu S, Moncalvo J-M, Lamoureux Y (2018) Validation of two Amanita species from eastern North America: A. rhacopus sp. nov. and A. variicolor sp. nov. MycoKeys 38: 47-57. https://doi.org/10.3897/mycokeys.38.27041
No full textFigure 1 Amanitarhacopus. a–c Basidiomes a CMMF002171(holotype), photograph by Yves Lamoureux b CMMF009640, photograph by Jacqueline Labrecque c HL016, photograph by Herman Lambert d–h Drawings of typical microscopic structures by Guy Fortin d Basidiospores e Basidia f Acrophysalides g Universal veil. h. Caulocystides. Scale bar: 3 cm (a, b), 10 µm (d, e), 20 µm (f–h)
Chromatin remodelling and histones mRNA accumulation in bovine germinal vesicle oocyte
No full textIn several mammalian species, a major remodelling of the germinal vesicle chromatin is known to occur towards the end of the oocyte growth. Various chromatin configurations have been identified. However, the mechanisms involved in this remodelling process are yet not completely understood. In the bovine species, four distinct and progressive states of chromatin compaction have been characterized and are linked to a gradual acquisition of the developmental potential.
Germinal vesicle oocytes were collected and separated in four groups according to their degree of chromatin condensation, ranging from a diffused state to a fully compacted configuration. To better understand the molecular changes undergoing in the oocyte during that critical period, transcriptomic analysis was performed with the EmbryoGENE microarray platform (custom Agilent 44K) in order to identify mRNA modulations occurring during the remodelling process.
An important proportion of genes showed a reduced mRNA level as the chromatin becomes more compacted, which strongly correlates with the decreased transcriptional activity at the end of oocyte growth. However, among the transcripts presenting an increased mRNA level, many were associated with the histone genes. Depending on the specific histone (H2A, H2B, H3, H4 or linker H1), an important mRNA accumulation occurs in the oocyte before ovulation. This dataset then offers a unique opportunity to picture the stock of accumulated histone mRNAs either to complete the build-up of a compacted chromatin, but also to ensure the protamine-histone replacement following fertilization and the completion of the first three cell cycles
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