348 research outputs found
Differential sexual network connectivity offers a parsimonious explanation for population-level variations in the prevalence of bacterial vaginosis: a data-driven, model-supported hypothesis
No full textCITATION: Kenyon, C. R., Delva, W. & Brotman, R. M. 2019. Differential sexual network connectivity
offers a parsimonious explanation for
population-level variations in the
prevalence of bacterial vaginosis : a datadriven,
model-supported hypothesis. BMC Women's Health, 19:8, doi:10.1186/s12905-018-0703-0.The original publication is available at https://bmcwomenshealth.biomedcentral.comBackground: The prevalence of bacterial vaginosis (BV) and vaginal microbiota types varies dramatically between
different populations around the world. Understanding what underpins these differences is important, as highdiversity
microbiotas associated with BV are implicated in adverse pregnancy outcomes and enhanced susceptibility
to and transmission of sexually transmitted infections.
Main text: We hypothesize that these variations in the vaginal microbiota can, in part, be explained by variations in
the connectivity of sexual networks. We argue: 1) Couple-level data suggest that BV-associated bacteria can be
sexually transmitted and hence high sexual network connectivity would be expected to promote the spread of BVassociated
bacteria. Epidemiological studies have found positive associations between indicators of network
connectivity and the prevalence of BV; 2) The relationship between BV prevalence and STI incidence/prevalence can
be parsimoniously explained by differential network connectivity; 3) Studies from other mammals are generally
supportive of the association between network connectivity and high-diversity vaginal microbiota.
Conclusion: To test this hypothesis, we propose a combination of empirical and simulation-based study designs.https://bmcwomenshealth.biomedcentral.com/articles/10.1186/s12905-018-0703-0Publisher's versio
Du purgatoire. La pitoiable voix de Rama. L'Eglise catholique, apostolique et romaine, la belle Rachel : ses prières, ses aumosnes, ses dons, ses sacrifices qu'elle offre incessamment pour les esprits des morts : ensemble la defence de ses larmes contre la pensée profane & impitoiable du Sieur Henry Chroüet Ministre de la Synagogue de Calvin étably au band d'Aulne
No full textEmpreinte : d,r. utur ezce cene (3) 1656 (R)
Porte la mention : "Avec permission des Superieurs"
Signatures : A-Z⁴ 2A-2F⁴ 2G²
Références : Theux, X. de. Bibliographie liégeoise(2e éd.) 210Source d'acquisition : R4236B Legs Baron A. Wittert 1903
Propriété et historique de la conservation : R4236B "Ex libris Joannis de Fraipont"Par Antoine Delva pasteur d'Aulne, au terroir d'Outre-Meus
Intralymphocyte free magnesium and calcium and insulin tolerance test in a group of essential hypertensive patients.
No full textIn order to assess the links which are claimed to exist between peripheral insulin resistance and intracellular magnesium and calcium concentrations, we measured free intralymphocyte magnesium (Mg(i)) and calcium (Ca(i)) concentrations as well as the rate constant of plasma glucose disappearance (K(itt)) after insulin injection (insulin tolerance test: ITT) in a group of 16 normotensive control subjects (NC) and 34 essential hypertensive subjects (EH). Mg(i) and Ca(i) were measured in triplicate by means of a fluorimetric technique based on the dyes furaptra and fura-2 respectively. K(itt) values proved significantly reduced in EH as compared to NC (M +/- SD, EH: 4.49 +/- 1.31 vs 5.28 +/- 1.19, P <0.05; 95% confidence limits: 0.231.5). Mg(i) and Ca(i) were not statistically different in EH as compared to NC subjects (Mg(i), NC: 266 +/- 20 mumol/l; EH: 245 +/- 50 mumol/l; Ca(i), NC: 47 +/- 9 nmol/l, EH: 46 +/- 13 nmol/l). We found a statistically significant inverse correlation in the whole study group between K(itt) and body mass index (R= -0.363, P<0.01) and a statistically significant positive correlation between K(itt) and Mg(i) (R= 0.347, P=0.013) was found. In a step-up multivariate regression analysis including blood pressure, plasma lipids, BMI, plasma magnesium, fasting insulin, fasting glucose, Mg(i) and Ca(i), the dependent variable K(itt) is statistically significantly correlated with body mass index and Mg(i). In a first attempt to study the relationships between insulin resistance, Mg(i) and Ca(i) in nucleated cells, the chosen index of peripheral resistance seems to be linked to intracellular free magnesium
Measurement by bioluminescence technique of erythrocyte membrane Na+,K+-ATPase activity in hypertensive patients
No full textErythrocyte membrane Na+,K+-ATPase activity was measured using a bioluminescence technique in 28 hypertensive patients (24 with essential hypertension, 2 with renovascular hypertension and 2 with hypertension secondary to primary hyperaldosteronism) and in 28 normotensive control subjects matched for age and sex. Erythrocyte Na+,K+-ATPase activity was significantly reduced in the patients with essential hypertension (130.9 +/- 11.4 vs. 186.6 +/- 19.5 nmol ATP/mg prot per h; mean values +/- SEM; p less than 0.05) and in the patients with secondary hypertension. A significant negative correlation was found between erythrocyte Na+,K+-ATPase and systolic blood pressure (r = -0.603; p less than 0.01), but not between Na+,K+-ATPase and plasma renin activity or plasma aldosterone levels. These data confirm the findings of a number of previous studies reporting reduced activity of erythrocyte Na+,K+-ATPase possibly related to the presence of a circulatory inhibitor of sodium pump. The method, based on ATP assay by bioluminescence, presents a high degree of specificity as well as simple, rapid execution
Endothelial progenitor cells and aptoptosis in patients with heart failure
No full textObjective: One the main features of heart failure is endothelial disfunction and some authors claimed this is caused by endothelial cell apoptosis. Scope of the study is to evaluate in a group of patients with heart failure the number of EPC (endothelial progenitor cells) both ex vivo and in culture in parallel with the assessment of EPC apoptosis and the echocardiographic evaluation of systolic and diastolic left ventricular function.
Design and Method: We studied 30 patients with congestive heart failure (CHF) and 16 healthy control subjects CS) by measuring the number of EPC both ex vivo and after 4 days in culture. We measured also EPC apoptosis together with echocardiographic parameters of systolic and diastolic left ventricular function.
Results and Conclusion: The EPC count measured both ex vivo (CD34+/KDR+; M ± ES, CHF, 9.5 ± 1.1%, CS, 5.1 ± 0.8%, p < 0.02) and after culture (KDR+/CD34+; M ± ES, CHF, 51.2 ± 6.6%; CS, 23.5 ± 8.8%, p < 0.0001) was significantly increased in CHF patients as compared to CS. In CHF patients EPC number measured in culture is directly correlated with hs-CRP (p = 0.035, r = 0.497). The EPC apoptosis does not show any statistically difference between CHF patients and CS. In CHF patients the number of apoptotic EPC was inversely correlated with total (r = −0.418, p = 0.02) and LDL cholesterol (r = −0.399, p = 0.03). We hypnotize that the increased oxidative stress characterizing heart failure may explain the increased EPC number which may have a compensatory significance for endothelial dysfunction as EPC number is directly linked to a crucial flogistic parameter as CRP. EPC apoptosis is not altered in CHF patients and does not seem to be related with the increased EPC number. The inverse correlation between EPC apoptosis and LDL cholesterol may represent the clinical equivalent of the in vitro ability of oxidized LDL to inhibit the macrophage binding to apoptotic endothelial cells
Intralymphocyte free magnesium and plasma triglycerides
No full textTo evaluate the relative effect of hypertension and plasma triglycerides on intralymphocyte magnesium we measured ionized intralymphocyte magnesium (Mg(i)) concentration by means of a fluorimetric method based on the dye Furaptra in 4 groups of subjects: 18 normotensive normotrigtyceridemic controls (NTNC), 9 hypertriglyceridemic normotensive patients (HTN), 8 hypertriglyceridemic essential hypertensive patients (HTEH), 17 normotriglyceridemic essential hypertensive patients (NTEH). Hypercholesterolemic, diabetic patients and alcoholics were excluded from the study. Mg(i) was found to be statistically reduced (ANOVA test F= 10.41, P=0.0001) in both HTN and HTEH (M+/- SD, HTN: 0.235 +/- 0.01, HTEH: 0.236 +/- 0.01 mmol/l) as compared to both NTNC and NTEH (M +/- SD, NTNC: 0.294 +/- 0.008, NTEH: 0.297+/- 0.009 mmol/l). A statistically significant negative correlation was found in the population as a whole between Mg(i) and plasma triglycerides (n=52, R= -541, P=0.00004). Our data suggest that hypertriglyceridemia per se and possibly the so-called plurimetabolic syndrome is characterized by low intralymphocyte free magnesium
Intralymphocyte Free Magnesium in a group of subjects with essential hypertension.
No full textDespite the importance of magnesium in essential hypertension, few data are available on the ionized intracellular concentration of this ion. We therefore studied intralymphocyte free intracellular magnesium (Mgi) in 32 untreated essential hypertensive subjects and 27 normotensive control subjects by means of a fluorimetric technique based on the use of the new magnesium-sensitive dye furaptra. We also measured intralymphocyte ionized calcium (Cai) with fura 2. No statistically significant differences were found in Mgi in hypertensive compared with normotensive subjects (essential hypertensive, 0.291 +/- 0.053 mmol/L; normotensive, 0.293 +/- 0.043 [mean +/- SD]). A statistically significant inverse correlation was established between Mgi and plasma triglycerides in essential hypertensive subjects (r = -.521, P = .002). The hypertensive group was arbitrarily divided into two subgroups according to plasma triglyceride levels (> 2 [n = 10] or < 2 mmol/L [n = 22]), and Mgi proved to be significantly lower in the subgroup with high plasma triglyceride levels compared with either the subgroup with normal triglycerides (P = .009; 95% confidence interval, 0.013-0.088) or the normotensive control group as a whole (P = .03; 95% confidence interval, 0.003-0.069) (high-triglyceride hypertensive subgroup, Mgi = 0.256 +/- 0.045 mmol/L; normal-triglyceride hypertensive subgroup, Mgi = 0.307 +/- 0.049). No statistically significant differences were found in Cai in hypertensive compared with normotensive subjects (hypertensive, 53 +/- 12 nmol/L; normotensive, 54 +/- 14). We did not find statistically significant correlations between Cai and plasma triglycerides, nor did we find any differences in Cai between the subgroup of hypertensive subjects with high plasma triglyceride levels and either the subgroup of hypertensive subjects with normal triglycerides or the normotensive control group as a whole. The discrepancies between our results in lymphocytes and data relating to either erythrocytes or platelets emphasize the need for caution before the results are extrapolated from one tissue to the other. The decreased Mgi levels in the subgroup of high-triglyceride hypertensive subjects may suggest a role for magnesium in plurimetabolic syndrome
Le noviciat réformé dressé à Aulne par Henry Chroüet ministre de Calvin : battu en ruine par Antoine Delva pasteur d'Aulne, au terroir de Dahlem, païs d'Outre-meuse. Partie premiere contenante fondamentalement l'estat du dessi fameux, ventilant entre lesdits pasteurs, & ministre dudit Aulne.
No full textEmpreinte : ladu e.A, n.ué coqu (3) 1657 (R)
Signatures : a⁸ A-Z⁸ 2A-2L⁸ 2M⁴
Références : De Theux, 2e éd. 21
Assessing the uncertainty around age-mixing patterns in HIV transmission inferred from phylogenetic trees
No full textUnderstanding age-mixing patterns in Human Immunodeficiency Virus (HIV) transmission networks can enhance the design and implementation of HIV prevention strategies in sub-Saharan Africa. Due to ethical consideration, it is less likely possible to conduct a benchmark study to assess which sampling strategy, and sub-optimal sampling coverage which can yield best estimates for these patterns. We conducted a simulation study, using phylogenetic trees to infer estimates of age-mixing patterns in HIV transmission, through the computation of proportions of pairings between men and women, who were phylogenetically linked across different age groups (15-24 years, 25-39 years, and 40-49 years); and the means, and standard deviations of their age difference. We investigated also the uncertainty around these estimates as a function of the sampling coverage in four sampling strategies: when missing sequence data were missing completely at random (MCAR), and missing at random (MAR) with at most 30%-50%-70% of women in different age groups being in the sample. The results suggested that age-mixing patterns in HIV transmission can be unveiled from proportions of phylogenetic pairings between men and women across age groups; and the mean, and standard deviation of their age difference. A 55% sampling coverage was sufficient to provide the best values of estimates of age-mixing patterns in HIV transmission with MCAR scenario. But we should be cautious in interpreting proportions of men phylogenetically linked to women because they may be overestimated or underestimated, even at higher sampling coverage. The findings showed that, MCAR was the best sampling strategy. This means, it is advisable not to use sequence data collected in settings where we can find a systematic imbalance of age and gender to investigate age-mixing in HIV transmission. If not possible, ensure to take into consideration the imbalance in interpreting the results.This research was funded by NRF-TWAS grant number 100014 awarded to DN. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Niyukuri, D (corresponding author), Stellenbosch Univ, Div Epidemiol & Biostat, Fac Med & Hlth Sci, Cape Town, South Africa ; Stellenbosch Univ, South African Dept Sci & Technol, Natl Res Fdn DST NRF, Ctr Excellence Epidemiol Modelling & Anal SACEMA, Cape Town, South Africa.
[email protected]
Inleiding tot het recht en privaat recht.
No full textOp basis van Willy Delva, Overzicht van het Belgisch burgerlijk recht en burgerlijk procesrech
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