946 research outputs found

    BONE MARROW TRANSPLANTATION FOR APLASTIC ANEMIA ASSOCIATED WITH DYSKERATOSIS CONGENITA

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    A 4 1/2-year-old girl developed severe aplastic anemia in association with dyskeratosis congenita. She was treated by allogeneic bone marrow transplantation following immunosuppression which included high-dose cyclophosphamide. Her clinical course following transplantation was complicated by severe graft-versus-host disease and gastrointestinal toxicity resembling that observed in patients with Fanconi's anemia treated by marrow transplantation. Two other patients with aplastic anemia and dyskeratosis congenita have been reported to develop similar transplant complications. Children with dyskeratosis congenita may be predisposed to the same complications as children with Fanconi's anemia following bone marrow transplantation and require preparative immunosuppressive regimens that do not include high-dose alkylating agents

    Influence of temperature on biofilm formation by Listeria monocytogenes on various food-contact surfaces, relationship with motility and cell surface hydrophobicity

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    Aims: To assess the ability of Listeria monocytogenes to form biofilm on differentfood-contact surfaces with regard to different temperatures, cellular hydrophobicityand motility.Methods and Results: Forty-four L. monocytogenes strains from food and foodenvironment were tested for biofilm formation by crystal violet staining. Biofilmlevels were significantly higher on glass at 4, 12 and 22C, as comparedwith polystyrene and stainless steel. At 37C, L. monocytogenes produced biofilmat significantly higher levels on glass and stainless steel, as compared withpolystyrene. Hydrophobicity was significantly (P < 0Æ05) higher at 37C than at4, 12 and 22C. Thirty (68Æ2%) of 44 strains tested showed swimming at 22Cand 4 (9Æ1%) of those were also motile at 12C. No correlation was observedbetween swimming and biofilm production.Conclusions: L. monocytogenes can adhere to and form biofilms on food-processingsurfaces. Biofilm formation is significantly influenced by temperature,probably modifying cell surface hydrophobicity.Significance and Impacts of the Study: Biofilm formation creates major problemsin the food industry because it may represent an important source of foodcontamination. Our results are therefore important in finding ways to preventcontamination because they contribute to a better understanding on howL. monocytogenes can establish biofilms in food industry and therefore survivein the processing environment.[...

    Influence of temperature on biofilm formation by Listeria monocytogenes on various food-contact surfaces: relationship with motility and cell surface hydrophobicity.

    No full text
    Aims: To assess the ability of Listeria monocytogenes to form biofilm on different food-contact surfaces with regard to different temperatures, cellular hydrophobicity and motility. Methods and Results: Forty-four L. monocytogenes strains from food and food environment were tested for biofilm formation by crystal violet staining. Biofilm levels were significantly higher on glass at 4, 12 and 22C, as compared with polystyrene and stainless steel. At 37C, L. monocytogenes produced biofilm at significantly higher levels on glass and stainless steel, as compared with polystyrene. Hydrophobicity was significantly (P < 0Æ05) higher at 37C than at 4, 12 and 22C. Thirty (68Æ2%) of 44 strains tested showed swimming at 22C and 4 (9Æ1%) of those were also motile at 12C. No correlation was observed between swimming and biofilm production. Conclusions: L. monocytogenes can adhere to and form biofilms on food-processing surfaces. Biofilm formation is significantly influenced by temperature, probably modifying cell surface hydrophobicity. Significance and Impacts of the Study: Biofilm formation creates major problems in the food industry because it may represent an important source of food contamination. Our results are therefore important in finding ways to prevent contamination because they contribute to a better understanding on how L. monocytogenes can establish biofilms in food industry and therefore survive in the processing environment
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