4,572 research outputs found
Erratum: Patients with Severe Obesity during the COVID- 19 Pandemic: How to Maintain an Adequate Multidisciplinary Nutritional Rehabilitation Program? (Obes Facts. (2021) DOI: 10.1159/000513283)
No full textIn the article by De Amicis et al. entitled "Patients with Severe Obesity during the COVID- 19 Pandemic: How to Maintain an Adequate Multidisciplinary Nutritional Rehabilitation Program?" [Obes Facts. 2021, DOI: 10.1159/000513283], the author list is incorrect. The correct author list is: De Amicis R. Cancello R. Capodaglio P. Gobbi M. Brunani A. Gilardini L. Castelnuovo G. Molinari E. Barbieri V. Mambrini S.P. Battezzati A. Bertoli S
Molinari Remo
No full textMolinari Remo: biografia e impegno educativo nell'ambito della scautismo italiano alle origini
Francophonie(s) en perspective(s)
No full textIn this contribution, we approach the French language through the prism of its diversity, with the aim of showing the notion of Francophonie in a "different" - diversitarian - light, by means of two mirrors: pronunciation and lexicon. We hope then to outline both heuristic and methodological perspectives, so that the francophone phenomenon can be apprehended in a plural and inclusive wa
Asymmetric reduction by transition metals complexes and biocatalysts
No full textIn the last years the importance of α- and -amnimoacids is increased since these molecules are interesting synthons for the preparation of unnatural peptides and bioactive compounds, in particular antibiotics or anticancer.
They can be easily obtained in optically pure form by reduction using metal catalyzed asymmetric hydrogenation1(chemocatalysis) or by enzyme mediated reduction2 (biocatalysis). In particular with the biocatalytical approach we used whole cells of different microorganisms endowed with enantioselective dehydrogenases, in chemocatalysis we used essentially Ir(I) and Ru(II) complexes of different atropoisomeric diphosphines.
In both cases our research efforts let us to obtain chiral enriched unnatural aminoacids by reduction of prochiral ketones3 and by reduction of tetra substituted double bonds4, which are generally very low active. Our research will show that the process robustness and stereochemical outcome is strongly dependent on thermodynamic parameters but also on the presence of additives.
We will discuss in detail nature and function of such compounds and we will demonstrate that it is possible to combine metal enantioselective reduction with stereoselective biocatalytic one.
1. E. Cesarotti; I. Rimoldi, P. Spalluto, F. Demartin Tetrahedron Asymmetry. 2007, 18, 1278-1283.
2. R. Gandolfi, E. Cesarotti, F. Molinari; D. Romano; I. Rimoldi Tetrahedron Asymmetry. 2009, 20, 411-414.
3. I. Rimoldi, E. Cesarotti, D. Zerla, F. Molinari, D. Albanese, R. Gandolfi submitted
4. R. Gandolfi, E. Cesarotti, D. Zerla, F. Molinari, I. Rimoldi submitte
Il lavoro del dramaturg. Nel teatro dei testi con le ruote
No full textPer la prima volta la figura e l'attività del dramaturg vengono affrontate "sistematicamente", in prospettiva storica e dall'interno del lavoro artistico. Mappe, orientamenti e crocevia storici accompagnano e disegnano la metamorfosi di una figura identificata con felice empatia artistica nell'attore ombra di un teatro che fa della capacità di trasformazione e di riviviscenza dei testi un punto di forza della propria identità. Dalla Germania all'area italofrancese, nella prima parte C. Meldolesi mette a fuoco epicentri, teorici e produttivi, del lavoro del dramaturg, indagandone principi operativi e fattori generalizzanti, con particolare attenzione ai risultati che fanno storia, dal lavoro di Heiner Muller su Brecht, a quello di Carrière per Brook. Lo stesso procedimento accompagna l'analisi del polo italiano. Anche qui esempi e risultati che hanno fatto - e fanno - storia (basti pensare al binomio Gerardo Guerrieri-Luchino Visconti). Nella seconda parte Renata M. Molinari propone gli snodi del percorso che l'ha fatta dramaturg
William R. Molinari
No full textPresident and chief operating officer of VanKampen Merritt, Inc., since 1990, Bill Molinari joined the Xerox Financial Services Company in 1977. The company provides wholesale investment brokerage and consulting services nation-wide, and also sponsors individual investments in tax-free trusts and mutual funds. He also serves on the Board of Governors for the Investment Company Institute. A business administration major, Molinari served Kappa Alpha Psi, professional business fraternity, as vice president. He earned a master of business administration degree in 1976 at Illinois State University. He was a three-year letterman in basketball and a standout as the sixth man on the 1975 CCIW championship team that featured Sikma, Spear, Foote, Bradley and Eldredge. He returned to assist Coach Dennie Bridges during the 1975-76 season as junior varsity coach, then moved to the University of Illinois at Champaign-Urbana as a part-time assistant to coach Lou Henson during 1976-77. Molinari has served Wesleyan as a class agent in the late 1970\u27s, is currently a member of the President\u27s Club, and works closely with the Career Center to facilitate student internships at Van Kampen Merritt.https://digitalcommons.iwu.edu/awards_distinguished/1063/thumbnail.jp
Enzymatic synthesis of (S)-1,2-O-isopropylidene glycerol
No full textThis invention relates to a process for the synthesis of (S)-1,2-O-isopropylidene glycerol by hydrolysis of a racemic mixture of (R,S)-1,2-O-isopropylidene glycerol acetate catalysed by Kluyveromyces marxianus, recovery of unreacted (R)-1,2-O-isopropylidene glycerol acetate, and hydrolysis thereo
Novel enantioselective enzymes from non-conventional yeasts
No full textThe major goal of my research is the development of synthetically useful selective biotransformations using “new” microbial enzymes by combining natural and non-natural conditions.
In this poster will be presented results from research activities on three different class of enzymes:
● Carboxylesterases – A new cell-bound carboxylesterase from Kluyveromyces marxianus have been purified and partially characterised. It has been employed for different enantioselective hydrolysis of chiral esters (i.e. solketal and aminoacid esters), not easily and/or efficiently resolved with commercial enzymes. Different strategies of optimisation and development of processes yelding multi-gram scale production will be also discussed.
Ref. Daniela Monti, Erica Elisa Ferrandi, Monica Righi, Diego Romano, Francesco Molinari. Purification and characterization of the enantioselective esterase from Kluyveromyces marxianus CBS 1553. Journal of Biotechnology (2008), 133(1), 65-72.
● Dehydrogenases - Pichia glucozyma produces DHs able to reduce aromatic ketones with peculiar stereodifferentiation between the prostereogenic faces of the carbonyls. Different aromatic substrates were reduced with high molar conversions and excellent stereoselectivity; the stereoselectivity was often complementary to the one observed with organometallics. The presence of different dehydrogenases in Pichia glucozyma allowed also for the deracemization of different racemic benzoins and α-substituted carbonyls.
Ref. Syed Masood Husain, Thomas Stillger, Pascal Dünkelmann, Melanie Lödige, Lydia Walter, Elke Breitling, Martina Pohl, Mara Bürchner, Ingo Krossing, Michael Müller, Diego Romano, Francesco Molinari, Stereoselective reduction of 2-hydroxy ketones towards syn- and anti-1,2-Diols – Accepted for publication in Advanched Sinthesys and Catalisys
R. Gandolfi, E. Cesarotti, F. Molinari, D. Romano, I. Rimoldi. Asymmetric reductions of ethyl 2-(benzamidomethyl)-3-oxobutanoate by yeasts
Tetrahedron: Asymmetry 2009, 20, 411-414.
● Enoate Reductases (OYEs) - Old Yellow Enzymes are flavin-dependent oxidoreductases that catalyze the stereoselective trans-hydrogenation of electron-poor alkenes (enoate reductase activity), which can result in important chiral synthons toward synthesis of many pharmaceuticals and chemicals. In this study, environmental yeasts have been screened for OYE activity with the purpose to develop new enoate reductase biocatalyst. Recombinant strains with enhanced OYE activity have been obtained and could be exploited for production of purified enzyme. Furthermore, the data herein reported attract interest due to promising applications in asymmetric synthesis.
Ref. Stefano Raimondi, Diego Romano, Alberto Amaretti, Francesco Molinari and Maddalena Rossi. Enoate reductases from non conventional yeasts as novel biocatalysts: cloning and functional expression in Saccharomyces cerevisiae.
Submitted at Journal of Biotechnolog
Resolution of (R,S)-2-phenylpropanoic acid by enantioselective esterification with dry microbial cells in organic solvent
No full textThe microbial direct esterification of racemic 2-phenyl-1-propanoic acid with ethanol by lyophilized mycelium of Aspergillus oryzae MIM and Rhizopus oryzae CBS 112.07 in organic solvents has been investigated. Dry cells of Rhizopus oryzae CBS 112.07 gave the (R)-ethyl ester with high enantiomeric excess (>97%) when the biotransformation was carried out in heptane or pentadecane. Dry mycelium of Aspergillus oryzae MIM furnished the ethyl ester of (S)-(+)-2-phenylpropanoic acid with 90% enantiomeric excess when used in toluene in a temperature range of 20-40°C
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