9,580 research outputs found
Buccione, R., Rizzo, G., Bloise, A., Calabrese, E., Mongelli, G., 2024. The Pollino UNESCO Global Geopark (southern Italy): a route in the Ancient Tethys Ocean. 37th International Geological Congress 2024, Busan (Korea).
No full text
DYNAMICS CHANGES IN ENDOTHELIAL EXTRACELLULAR MATRIX INDUCED BY VERY LOW DENSITY LIPOPROTEIN
No full textPreviously we described the vascular extracellular matrix remodeling
induced by normal VLDL at physiological levels
1) Particularly, we described the differences in chondroitin sulfate/
dermatan sulfate proteoglycans (CS/DS-PGs) according to
the endothelial cell phenotypes. 2) The aim of the present study was to analyze the expression
pattern of CS/DS-PGs in the presence of increasing levels of
N-VLDL. Human N-VLDL were isolated by ultracentrifugation
from healthy volunteers. Human umbilical vein endothelial
cells (HlNEC) were obtained and cultured as described by Ulrich-
Merzenic.
3) Then, HlNEC were incubated with 0,75 and 100 mcg/mL of
lipoprotein for 24 h.
Protocols were approved by the Bioethics Committee of the University
of Buenos Aires, Argentina. After treatment, CS/DS-PGs
were characterized through:
1) PG core protein secretion, specifically decorin, biglycan, and
versican analysis by immunoblot;
2) glycosaminoglycans (GAGs) content studied by reverse phase
HPLC;
3) the levels of chondroitin N-acetylgalactosaminyltransferase-2
(ChGn-2) and chondroitin-4-0-sulfotransferase-1 (C4ST-1)
mRNA by RT-PCR.
A significant increase in the protein core of decorin and biglycan
was detected after treatment (0 vs 75 and 0 vs 100 mcg/mL
N-VLDL,p<0.05, n=3), whereas for versican the increase was only
observed at 75 mcg/mL (0 vs 75 mcg/mL N-VLDL,p<O.OOl,n=3).
A significant increase in CS and DS chains was detected at both
levels of N-VLDL (38.5±15.0; 388.0±20.0 and 82.5±50.0 ng/mL; 0,
75 and 100 mcg/mL N-VLDL,p<0.05, n=3), accompanied by an increase
in the sulfation ratio 4S/0S of CS and DS chains (4.88±0.13;
13.97±1.8; 14.53±11.46; 0, 75 y 100 mcg/mL, n=3). No differences
were observed in ChGn-2 and C4ST-1. At physiological levels,
VLDL induced a CS/DS-PG secretion pattern that may contribute
to the atheroprotective properties of this endothelial phenotype;
such characteristics were lost in the presence of higher levels of
the lipoprotein. Our results highlight the importance of CS/DS
PGs as a new target for atherosclerosis treatment,
Bibliografia
1. Oberkersch R, Rasente Y, Barakian B., Yuschak S., Volpi N., Calabrese
G. Extracellular matrix remodeling of endothelial cell was induced by
very low density lipoproteins through NFKB activation. 28° Congresso
Nazionale della Societa ltaliana per 10 Studio dell'Arteriosclerosi. Roma,
Italia. November 23·25, 2014. .
2. Oberkersch R, Rasente Y, Gualco L, Yuschak S, Calabrese G. Very low
density lipoproteins induce differential vascular extracellular remodeling
according to the endothelial phenotype. Angiogenesis and Leukocytes
Atherosclerosis. Geneve, Switzerland. January 30-31, 2014.
3. Ulrich-Merzenich G, Metzner C, Bhonde R, MaIsch G, Schiermeyer
B, Vetter H. Simultaneous isolation of endothelial and smooth muscle
cells from human umbilical artery or vein and their growth response to
low-density lipoproteins
Author Correction: Gluten consumption and inflammation affect the development of celiac disease in at-risk children
No full textThe original version of this Article contained an error in the spelling of the authors Renata Auricchio, Ilaria Calabrese, Martina Galatola, Donatella Cielo, Fortunata Carbone, Marianna Mancuso, Giuseppe Matarese, Riccardo Troncone, Salvatore Auricchio & Luigi Greco which were incorrectly given as Auricchio Renata, Calabrese Ilaria, Galatola Martina, Cielo Donatella, Carbone Fortunata, Mancuso Marianna, Matarese Giuseppe, Troncone Riccardo, Auricchio Salvatore & Greco Luigi. The original article has been corrected
Financial constraints, R&D investment and uncertainty: new evidence from the Italian automotive supply chain
No full textCalabrese GG, Falavigna G, Ippoliti R. Financial constraints, R&D investment and uncertainty: new evidence from the Italian automotive supply chain. Technology Analysis & Strategic Management. 2023:1-13
Letter dated 21 October 1967 from Lorenzo A. Richards to Susan R. Calabrese of Riverside Public Utilities Department
No full textLetter dated 21 October 1967 from Lorenzo A. Richards in Riverside, California, to Susan R. Calabrese of Riverside Public Utilities Department regarding his application for appliance reimbursemen
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