6 research outputs found
Biochemical protein interactions of Gliotactin at the tricellular junction
No full textThe septate junction is an occluding junction in invertebrates, similar in function to tight
junctions, playing a role in epithelial barriers, and in apical/basal polarity. Septate junction
interactions are still being characterized as new component proteins are discovered. One septate
junction protein, Gliotactin, was discovered in Drosophila to correlate with the mislocalization
of characteristic septate junction proteins in a Gliotactin null animal. However, Gliotactin is the
only component found exclusively at the level of septate junctions at tricellular junctions in
epithelia. The tricellular junctions are the structure at the convergence of three cells and a
potential organizing factor of the septate junction. This led to the question, what is Gliotactin's
role in the organization of the tricellular and septate junctions? To study this, we looked at
Gliotactin interactors, and attempted to elucidate a model of tricellular and septate junction
protein interactions. Previous attempts at finding Gliotactin interactors were made using in vitro
systems or by using transgenic animals using an over-expressed epitope-tagged Gliotactin, that
showed that Gliotactin interacts with known septate junction proteins in a calcium dependent
manner. This thesis aims to further explore Gliotactin interactions with the hypothesis that
native Gliotactin in Drosophila interacts in a calcium dependant manner with septate junction
proteins, Discs Large, and Neurexin IV. Using co-immunoprecipitation and GST pulldowns, on
native Gliotactin protein in Drosophila, I have shown that Gliotactin does not interact with
Neurexin IV but does interact with Discs Large in a calcium-dependent manner. This is
significant in that, to date, there has been no known interactor of Discs Large or of native
Gliotactin at the tricellular junction. I also present data on unidentified potential Gliotactin
interactors seen in a GST pulldown assay. The data presented in this thesis has contributed to a
new working model of the tricellular junction and the role of Gliotactin.Science, Faculty ofZoology, Department ofGraduat
Propiedades psicométricas del inventario de violencia en las relaciones de pareja en los adolescentes (CADRI) del distrito de Salaverry-Trujillo
No full textLa presente investigación tiene como propósito presentar los resultados alcanzados
en el análisis de las Propiedades Psicométricas del Inventario de violencia en las
relaciones de pareja en los adolescentes (CADRI) del distrito de Salaverry-Trujillo,
cuya población estuvo conformada por 249 estudiantes de 15-19 años de edad de
6 Instituciones Educativas del Distrito de Salaverry, que mantenían o hayan
mantenido hasta hace 1 año atrás una relación de pareja. Se determinó la validez
de contenido bajo el criterio de 7 expertos, mostrando índices de .81 a 1.0. Mediante
correlación ítem total e ítem factor corregido se evidenció índices de homogeneidad
corregidos mediante el coeficiente de correlación de Pearson para el ítem y el total
con resultados de .18 y .59 en violencia cometida y de .21 y .69 para de violencia
sufrida. Para la confiabilidad se utilizó el método de consistencia interna mediante
el método alfa de Cronbach, observándose los totales de violencia cometida (.84;
IC: .81 -.87) y sufrida (.88; IC: .86 -.90) y una confiabilidad entre .52 y .79 en los
factores de violencia cometida y entre .53 y .82 en los factores de violencia sufrida.
Finalmente, se desarrollaron normas para la variable Violencia Cometida,
generales en los factores Violencia Relacional y Amenazas; para Violencia Sexual,
Verbal Emocional, Física y variable total, denotan diferencias por género. En
violencia Sufrida, baremos específicos sólo para el factor violencia física
Curating the innate immunity interactome
No full textpeer-reviewedBackground: The innate immune response is the first line of defence against invading pathogens and is regulated by complex signalling and transcriptional networks. Systems biology approaches promise to shed new light on the regulation of innate immunity through the analysis and modelling of these networks. A key initial step in this process is the contextual cataloguing of the components of this system and the molecular interactions that comprise these networks. InnateDB (http://www.innatedb.com) is a molecular interaction and pathway database developed to facilitate systems-level analyses of innate immunity. Results: Here, we describe the InnateDB curation project, which is manually annotating the human and mouse innate immunity interactome in rich contextual detail, and present our novel curation software system, which has been developed to ensure interactions are curated in a highly accurate and data-standards compliant manner. To date, over 13,000 interactions (protein, DNA and RNA) have been curated from the biomedical literature. Here, we present data, illustrating how InnateDB curation of the innate immunity interactome has greatly enhanced network and pathway annotation available for systems-level analysis and discuss the challenges that face such curation efforts. Significantly, we provide several lines of evidence that analysis of the innate immunity interactome has the potential to identify novel signalling, transcriptional and post-transcriptional regulators of innate immunity. Additionally, these analyses also provide insight into the cross-talk between innate immunity pathways and other biological processes, such as adaptive immunity, cancer and diabetes, and intriguingly, suggests links to other pathways, which as yet, have not been implicated in the innate immune response. Conclusions: In summary, curation of the InnateDB interactome provides a wealth of information to enable systems-level analysis of innate immunity.Genome BC - Pathogenomics of Innate Immunity (PI2) project; Foundation for the National Institutes of Health and the Canadian Institutes of Health Research - Grand Challenges in Global Health Research Initiative (Grand Challenges ID: 419); Teagasc; DJL was funded in part during this project by a postdoctoral trainee award from the Michael Smith Foundation for Health Research (MSFHR); FSLB is a Canadian Institutes of Health Research (CIHR) New Investigator and a MSFHR Senior Scholar; REWH holds a Canada Research Chair (CRC
InnateDB: facilitating systems-level analyses of the mammalian innate immune response
No full textAlthough considerable progress has beenmade in dissecting the signaling pathways involved in the innate immune response, it is now apparent that this response can no longer be productively thought of in terms of simple linear pathways. InnateDB (www.innatedb.ca) has been developed to facilitate systems-level analyses that will provide better insight into the complex networks of pathways and interactions that govern the innate immune response. InnateDB is a publicly available, manually curated, integrative biology database of the human and mouse molecules, experimentally verified interactions and pathways involved in innate immunity, along with centralized annotation on the broader human and mouse interactomes. To date, more than 3500 innate immunity-relevant interactions have been contextually annotated through the reviewof 1000 plus publications. Integrated into InnateDB are novel bioinformatics resources, including network visualization software, pathway analysis, orthologous interaction network construction and the ability to overlay user-supplied gene expression data in an intuitively displayed molecular interaction network and pathway context, which will enable biologists without a computationa
