1,721,470 research outputs found
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL): a proteiform neurological disease of expanding importance. Reasons for establishing and italian registry
No full textMolecular portraits: the evolution of the concept of transcriptome-based cancer signatures.
Full text linkCancer results from dysregulation of multiple steps of gene expression programs. We review how transcriptome profiling has been widely explored for cancer classification and biomarker discovery but resulted in limited clinical impact. Therefore, we discuss alternative and complementary omics approaches
Saccadic eye movements analysis as a measure of drug effect on central nervous system function
No full textDEFINING A NEURON: NEURONAL ELAV PROTEINS
No full textNeuronal cells strongly depend on the
control exerted by RNA-binding proteins (RBPs) on
gene expression for the establishment and maintenance
of their phenotype. Neuronal ELAV (nELAV)
proteins are RBPs able to influence virtually every
aspect of the postsynthesis fate of bound mRNAs,
from polyadenylation, alternative splicing and nuclear
export to cytoplasmic localization, stability and translation.
They enhance gene expression through the last
two, best documented activities, increasing mRNA
half-life and promoting protein synthesis by a stillunknown
molecular mechanism. Developmentally,
nELAV proteins have been shown to act as inducers
of the transition between neural stem/progenitor cells
and differentiation-committed cells, also assisting
these neuroblasts in the completion of their maturation
program. In brain physiology, they are also the
first RBPs demonstrated to have a pivotal role in
memory, where they probably control mRNA availability
for translation in subcellular domains, thereby
providing a biochemical means for selective increase
in synaptic strength
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Mobility Control Centre and Artificial Intelligence for Sustainable Urban Districts
Full text linkThe application of artificial intelligence (AI) to dynamic mobility management can support the achievement of efficiency and sustainability goals. AI can help to model alternative mobility system scenarios in real time (by processing big data from heterogeneous sources in a very short time) and to identify network and service configurations by comparing phenomena in similar contexts, as well as support the implementation of measures for managing demand that achieve sustainable goals. In this paper, an in-depth analysis of scenarios, with an IT (Information Technology) framework based on emerging technologies and AI to support sustainable and cooperative digital mobility, is provided. Therefore, the definition of the functional architecture of an AI-based mobility control centre is defined, and the process that has been implemented in a medium-large city is presented
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis
No full textTumour onset and progression are due to the accumulation of genomic lesions, which alter gene expression and ultimately proteome activities. These lesions are thought to affect primarily the transcriptional control of gene expression. In the present study, we aimed at evaluating the genome-wide occurrence of alterations in the translational control exploiting an isogenic, phenotypically validated cellular model of colorectal cancer (CRC) transition from invasive carcinoma to metastasis. In this model, microarray profiling shows that changes in the level of messenger ribonucleic acid (mRNA) association with polysomes occur more than 2-fold than changes in the level of total cellular mRNA. When common to both the total and polysomal compartments, these changes are also homodirectional, being amplified in magnitude at the polysomal level. Comparison between the transcriptional and the translational fluctuations revealed distinct signatures of statistically over-represented gene functions, involving the program of cell proliferation for both levels of analysis, while the apoptosis and the translation programs were affected mainly at translation. Looking for an upstream determinant of translational deregulation, we found an increase in the hyperphosphorylated form of the 4E-BP1 protein in the metastatic cell line, possibly resulting in an increased activation of cap-dependent translation due to increased activity of the eIF4E protein. Analysis of the distribution profiles for the 5' untranslated region (5'-UTR) length of the changed genes showed an association between longer 5'-UTRs and the probability for the relevant gene to be altered translationally, consistent with enhanced eIF4E function. This genome-wide analysis is in favour of a model of profound alteration of translational control in late CRC progression. It also suggests polysomal mRNA profiles as a new, informative dimension for the study of transcriptome imbalance in cancer
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