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EFFETTI DELL’ALIMENTAZIONE SULLA ATTIVITA’ PRODUTTIVA DI GALLI APPARTENENTI A DUE LINEE GENETICHE DIVERSE: PRIMI RISULTATI
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Simplified HPLC-UV method for the determination of alfa-tocopherol in plasma.
No full textVitamin E, known for its great nutritional importance, is normally included in animal diets as DL-tocopherol acetate.
The authors propose a method that makes it possible to determine the concentration of vitamin E in plasma without
saponification. This method enable to avoid aggressive treatments on the analyte and complex procedures; it detects
vitamin E only in form of DL-x-tocopherol.
Lipoproteins of analysed plasma were denaturised by methanol. Vitamin E was extracted by petroleum ether in presence
of NaCl. The extract was dried by rotavapor at 45 °C, solubilized by methanol and injected in HPLC (C18 column, reversed
phase). The quantitative determination was carried out by UV detector settled on 294 nm.
Tests of repeatability inter-analysis and intra-analysis gave coefficient of variability (CV%) respectively of 1.64 and
2.41%. The mean recovery was 100%
Effect of dried extracts of Aspergillus oryzae and their association with Saccharomyces cerevisiae on mid lactation dairy cattle productivity under moderate heat stress conditions.
No full textA trial was conducted in Parmigiano Reggiano area with the aim
to study the effects of Aspergillus Oryzae fermentation extract
pure or in association with Saccharomyces cerevisiae yeast as
direct feed microbials on the productivity and milk quality of
dairy cattle diet during moderate heat stress. Twelve Holstein
multiparous cows with average parity of 2.7 and average DIM of
193, were involved in sequential study design of three periods of
21 days including 14 days of adaptation and 7 days of measurements
and sampling. During the first period animals were treated
with 7 g/d of a commercial product containing 50% of
Saccharomyces cerevisiae yeast as direct feed microbials and
50% of fermentation extracts of Aspergillus oryzae –treatment
“SC+AO”-. During the second period animals were fed the base
diet –“CON”- while during the third period the diet included 7
g/cow/d of fermentation extracts of Aspergillus oryzae –treatment
“AO”-. Diet was composed of a mix of hays, straw, soybean
meal and a commercial protein mix including mineral and vitamin
supplement and was administered as Total Mixed Ration
(36.18% NDF, 14.18% CP, 24.07% Starch). Measurements included
environmental temperature and humidity (to calculate
Thermal Humidity Index –THI-), group feed intake and orts, individual
milk production (daily), BCS, fecal score (FS) and rectal
temperature (RT) (twice weekly). Sampling included diet collection,
individual faeces and individual milk (twice a week). Milk
composition, diet composition and indigestible NDF (uNDF) and
faeces uNDFwere determined to calculate in vivo NDF digestibility
(NDFD) and dry matter digestibility (DMD). Statistical analysis
of data was performed using the SPSS for windows (vers.21);
the repeated measures procedure of the GLM model was adopted
using THI as covariate. The treatment AO induced an increase of
dry matter intake (25.55 vs. 23.93 and 22.12 kg of CON and
SC+AO thesis; P<0.001), of in vivo NDFD (53.18 vs. 48.49 and
49.23 %NDF; P<0.05) compared to the other treatments and of in
vivo DMD with respect to the CON group (70.50 vs. 69.06;
P<0.05). AO has also been observed to decrease rectal temperature
(38.31 vs. 38.77 and 38.80°C; P<0.01). Energy Corrected
Milk production such as the percentage of protein and LDG
parameters (r and A30) appeared improved. Dried extracts of
Aspergillus orizae had some beneficial effects on DMI, showing
a higher activity if compared with SC+AO, but did not heavily
affected milk production and qualit
L'INTEGRAZIONE DELLE RAZIONI PER LE BOVINE DA LATTE CON VITAMINE A, D3 ED E IN FORMA RUMINO-PROTETTA. EFFETTI SULL'EFFICIENZA PRODUTTIVA E RIPRODUTTIVA (CONTRIBUTO SPERIMENTALE)
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