43,582 research outputs found
Recombinant production of Yersinia enterocolitica pyruvate kinase isoenzymes PykA and PykF
No full textThe glycolytic enzyme pyruvate kinase (PK) generates ATP from ADP through substrate-level phosphorylation powered by the conversion of phosphoenolpyruvate to pyruvate. In contrast to other bacteria, Enterobacteriaceae, such as pathogenic yersiniae, harbour two pyruvate kinases encoded by pykA and pykF. The individual roles of these isoenzymes are poorly understood. In an attempt to make the Yersinia enterocolitica pyruvate kinases PykA and PykF amenable to structural and functional characterisation, we produced them untagged in Escherichia coli and purified them to near homogeneity through a combination of ion exchange and size exclusion chromatography, yielding more than 180 mg per litre of batch culture. The solution structure of PykA and PykF was analysed through small angle X-ray scattering which revealed the formation of PykA and PykF tetramers and confirmed the binding of the allosteric effector fructose-1,6-bisphosphate (FBP) to PykF but not to PykA
Na rozdrożach współczesnej demokracji w kontekście partycypacji obywatelskiej młodzieży
No full textConsiderations included in the article by Robert Pyka concern tensions between a national country losing influence and still developing logic of the government of the globalised world. The crux of the matter is notions such as Pierr Rosanvalon’s “counter‑democracy”. The author asks about a contemporary sense of representative democracy, pointing to the contracting role of the country in the light of growing expectations of the citizens. Showing dangers coming from a global market, the sense of being lost in an abruptly changing reality, as well as alienation and the lack of understanding of actions of selected government representatives threaten with the arrival of post‑democracy in which the citizens stop treating a democratic system seriously and, in consequence, leave it. Critical considerations are confirmed by the author who quotes the studies pointing to the fall of election frequency among teenagers and a small engagement of the members of party organizations. An increase in a neutral or a negative attitude to institutionalized forms of representative democracy is accompanied by simultaneous searching for direct and more effective forms of political participation. In the end of the article Pyka admits that “democratic movements” are right believing that they will motivate the citizens to efficient actions shaping a new and better democracy in the 21st century
Author Tom Keneally back stage at the Nimrod Theatre, Sydney, 1980 /
No full textTitle from acquisitions documentation.; Part of the collection: Robert McFarlane collection of photographs.; Inscriptions: "Author Tom Keneally back stage Nimrod Theatre 1980 Robert McFarlane"--In pencil on reverse.; Also available online at: http://nla.gov.au/nla.pic-vn6615438
Author Tom Keneally and actor Justine Saunders backstage during the rehearsals of Bullie's House, Nimrod Theatre, Sydney, 1980 /
No full textTitle from acquisitions documentation.; Part of the collection: Robert McFarlane collection of photographs.; Inscriptions: "Author Tom Keneally + Actor Justine Saunders backstage Nimrod Theatre 1980 during rehearsal's 'Bulli'es House' Robert McFarlane"--In pencil on reverse.; Also available online at: http://nla.gov.au/nla.pic-vn6615450
Preface
No full textWstęp do książki "Regionalisation in Europe : the state of affairs" pod red. G. Libor, R. Pyka, D. Nowalska-Kapuści
Introduction
No full textWstęp do książki "Regionalisation in Europe : the state of affairs" pod red. G. Libor, R. Pyka, D. Nowalska-Kapuścik
Robert Sparks Walker diary, 1941
No full textDiary authored by Chattanooga author and naturalist, Robert Sparks Walker
Robert Sparks Walker diary, 1945
No full textDiary authored by Chattanooga author and naturalist, Robert Sparks Walker
Robert Sparks Walker diary, 1945
No full textDiary authored by Chattanooga author and naturalist, Robert Sparks Walker
Robert Sparks Walker diary, 1941
No full textDiary authored by Chattanooga author and naturalist, Robert Sparks Walker
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