1,660 research outputs found

    Sequence based DNA markers and genotyping for cereal genomics and breeding

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    The last three decades has seen the rapid evolution of a variety of DNA-based molecular markers that are powerful tools for genome analysis and marker-trait association (MTA) studies. Recently, high-throughput sequence-based methods have been developed for use in plant breeding. Sequence-based markers include simple sequence repeats (SSRs; also known as microsatellites) and single nucleotide polymorphisms (SNPs), which now dominate applications in modern genetic analysis. Insertion site-based polymorphisms (ISBPs), copy number variations (CNVs) and presence and absence variations (PAVs) constitute another group of markers that are being applied in a variety of plant systems. Markers may be used for a variety of purposes including diversity analysis, linkage-based QTL mapping, LD-based association mapping and marker assisted selection (MAS). Large sequence datasets (including both, genomic sequences and expressed sequences) are available for many cereals, enabling the mining for large numbers of SSRs, SNPs, ISBPs and CNVs/PAVs. Recombination bins are being used as markers for genotyping mapping populations and QTL analysis in crops such as rice, where reference genome sequences are available. In this chapter we describe the discovery and application of molecular markers using automated sequencing platforms including those based on next generation sequencing (NGS)

    Erratum to: Is Sensory Loss an Understudied Risk Factor for Frailty? A Systematic Review and Meta-analysis

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    In the article “Is Sensory Loss an Understudied Risk Factor for Frailty? A Systematic Review and Meta-analysis,” an author was missing. Ana Maseda should be listed as the 11th author. The correct author list is: Benjamin Kye Jyn Tan, Ryan Eyn Kidd Man, Alfred Tau Liang Gan, Eva K Fenwick, Varshini Varadaraj, Bonnielin K Swenor, Preeti Gupta, Tien Yin Wong, Caterina Trevisan, Laura Lorenzo-López, Ana Maseda, José Carlos Millán-Calenti, Carla Helena Augustin Schwanke, Ann Liljas, Soham Al Snih, Yasuharu Tokuda, Ecosse Luc Lamoureux. This error has been corrected

    Engineering materials : research, applications and advances / author, K.M. Gupta.

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    "A CRC title."Includes bibliographical references and index.596 p.

    Selfcoelum mertensis Gupta 1970, n. comb.

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    S. mertensis (Gupta, 1970) n. comb. Type host. Common sandpiper, Actitis hypoleucos (Linnaeus) (Charadriiformes: Scolopacidae). Type locality. Merta, Nagaur District, Rajasthan, India. Remarks. This species was originally described as Ophthalmophagus mertensis Gupta, 1970. On the one hand, the position of the posttesticular ovary may have been confused with the “receptaculum seminis uterinum”, as shown in Fig. 3 of the original description and may be more posteriorly situated nearer to the posterior arch of the cyclocoel, which is more typically the case in members of this genus. On the other hand, the “receptaculum seminis uterinum” may be the posterior testis and the anterior testis which is shown as being situated near the midbody may be part of the uterus. If the second situation is true, then this species would have an intertesticular ovary froming a triangle with the diagonal testes and would be assigned to Cyclocoelinae. However, the species was described from a single damaged specimen and it appears that the anterior end is rotated somewhat to the left causing the cirrus sac to be shifted more laterally than normal. We assume that the author is correct and that the ovary is situated off the midline of the body some distance posterior to the anterior testis as is shown in Fig. 3 of the original description (apparently a dorsal view). It should be noted that although both the diameters of the oral sucker (200, but about 690 calculated using the scale provided) and the pharynx (200, but about 350 calculated using the scale provided) are given as being the same, the oral sucker is shown in Fig. 3 as being much wider than the pharynx (about twice as wide). The length of the intertesticular and posttesticular spaces could not be calculated from the original figures because of the lack of verifiable reference measurements and the scale provided. As originally described, the ovary is posttesticular forming an elongate triangle with the testes (Szidatitreminae), the genital pore is postpharyngeal and the vitelline fields are not confluent posteriorly, placing this species in Szidatitrema. Rudimentary oral sucker present—Gupta (1970).Published as part of Dronen, Norman O. & Blend, Charles K., 2015, Updated keys to the genera in the subfamilies of Cyclocoelidae Stossich, 1902, including a reconsideration of species assignments, species keys and the proposal of a new genus in Szidatitreminae Dronen, 2007, pp. 1-100 in Zootaxa 4053 (1) on page 87, DOI: 10.11646/zootaxa.4053.1.1, http://zenodo.org/record/23711

    Scientometric study of exploration geophysics-author productivity trends

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    145-152<span style="font-size:12.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-US">A cumulative index of geophysics for the two most important journals "Geophysics" and "Geophysical Prospecting" in the field of exploration geophysics for the period 1936-1985 was analyzed for author productivity trends. Author Productivity trends were tested for the application of Lotka's law by applying K-S statistical test. Two files were generated out of this data base; one for the period 1936-1985 and the other for the period 1936-1976 to test the time sensitivity of Lotka's law. Lotka's law did not apply as inverse square law but could apply satisfactorily with exponent value of 2. 1 on author productivity distribution patterns of both the files.</span

    Erratum: NuSTAR view of Be/X-ray binary pulsar 2S 1417–624 during 2018 giant outburst (Monthly Notices of the Royal Astronomical Society (2019) 490 (2458-2466) DOI: 10.1093/mnras/stz2795)

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    This is an erratum to the paper ‘NuSTAR view of Be/X-ray binary pulsar 2S 1417–624 during 2018 giant outburst’ (2019, MNRAS, 490, 2458–2466). In the original version of this article an affiliation of Shivangi Gupta was missing. This has now been corrected and the Indian Institute of Technology has been added. The author apologies for the error

    Transported PDF Modeling of Jet-in-Hot-Coflow Flames

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    A probability density function (PDF)-based combustion modeling approach for RANS simulation of a jet issuing into a hot and diluted coflow is performed. A tabulated chemistry-based model, i.e., flamelet-generated manifold (FGM), is adopted in the PDF method. The manifolds are constructed using igniting counterflow diffusion flamelets with different coflow compositions. To handle the inhomogeneity of the coflow and the entrainment of the ambient air, a second mixture fraction is defined to quantify the mixing of a representative coflow composition with the ambient air. The chemistry is then parameterized as a function of two mixture fractions and a reaction progress variable. To assess the modeling approach, Adelaide JHC flames, namely HM1, HM2, and HM3, having different oxygen concentrations in the hot coflow, 3%, 6%, and 9% O2, respectively, have been simulated for Reynolds number (Re) = 10,000. Profiles of mean mixture fraction and major species are accurately captured by the model along with the mean temperature. The mean temperature profiles are also captured nicely, while the sensitivity of progress variable (PV) on the predictions is highlighted.Accepted Author ManuscriptFluid Mechanic

    Single-molecule DNA sequencing technologies for future genomics research

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    During the current genomics revolution, the genomes of a large number of living organisms have been fully sequenced. However, with the advent of new sequencing technologies, genomics research is now at the threshold of a second revolution. Several second-generation sequencing platforms became available in 2007, but a further revolution in DNA resequencing technologies is being witnessed in 2008, with the launch of the first single-molecule DNA sequencer (Helicos Biosciences), which has already been used to resequence the genome of the M13 virus. This review discusses several single-molecule sequencing technologies that are expected to become available during the next few years and explains how they might impact on genomics research
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