1,720,972 research outputs found
Validation of a seminested PCR approach for rapid detection of Salmonella enterica subsp. enterica serovar Gallinarum
No full textSalmonella enterica subsp. enterica serovar Gallinarum (S. Gallinarum) is the causative agent of fowl typhoid,
one of the major causes of mortality and morbidity on poultry farms. Even though it has been substantially
eradicated in many developed countries, the disease still remains endemic in Central and South America, in
Africa and in the Mediterranean countries of Europe. This leads to the routine screening of flocks, mainly by
cultivation and serological techniques, which are expensive, as well as time and labour-consuming. Here we
describe a simple and specific PCR-based method for detecting S. Gallinarum. It relies on two seminested PCRs
which use four pairs of primers designed on the basis of two genomic regions which appear to be exclusive to
the pathogen. Furthermore, an internal positive control was devised in order to avoid any false negative
results. We performed sensitivity and specificity tests, and our findings showed the cogency of the system and
its potential effectiveness even for routine uses
[Radioimmunologic determination of blood insulin in the last weeks of pregnancy. Correlation between lipemic factors, blood glucose and immunoreactive insulinemia].
No full textMolecular characterization and antimicrobial susceptibility of Salmonella enteritidis and S. typhimurium isolated from laying hens in Southern Italy
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[Immuno-reactive insulin, glucose and lipids in the blood in the first weeks of pregnancy].
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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